Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2883 and EX28DRAFT_2884 are separated by 128 nucleotides EX28DRAFT_2884 and EX28DRAFT_2885 are separated by 13 nucleotides EX28DRAFT_2885 and EX28DRAFT_2886 are separated by 192 nucleotides
EX28DRAFT_2883: EX28DRAFT_2883 - Transcriptional regulator, at 189,786 to 190,703
_2883
EX28DRAFT_2884: EX28DRAFT_2884 - RNA, at 190,832 to 191,039
_2884
EX28DRAFT_2885: EX28DRAFT_2885 - Bacterial protein of unknown function (DUF903), at 191,053 to 191,280
_2885
EX28DRAFT_2886: EX28DRAFT_2886 - cysteine desulfurase, catalytic subunit CsdA, at 191,473 to 192,678
_2886
Position (kb) 190
191
192 Strain fitness (log2 ratio) -1
0
1
2 at 189.867 kb on - strand at 189.901 kb on + strand, within EX28DRAFT_2883 at 189.902 kb on - strand, within EX28DRAFT_2883 at 189.924 kb on + strand, within EX28DRAFT_2883 at 189.925 kb on - strand, within EX28DRAFT_2883 at 189.925 kb on - strand, within EX28DRAFT_2883 at 189.933 kb on - strand, within EX28DRAFT_2883 at 190.070 kb on + strand, within EX28DRAFT_2883 at 190.070 kb on + strand, within EX28DRAFT_2883 at 190.083 kb on + strand, within EX28DRAFT_2883 at 190.084 kb on - strand, within EX28DRAFT_2883 at 190.182 kb on + strand, within EX28DRAFT_2883 at 190.222 kb on + strand, within EX28DRAFT_2883 at 190.635 kb on - strand at 190.806 kb on + strand at 190.806 kb on + strand at 190.816 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.821 kb on - strand at 190.822 kb on + strand at 190.983 kb on + strand, within EX28DRAFT_2884 at 190.983 kb on + strand, within EX28DRAFT_2884 at 190.984 kb on - strand, within EX28DRAFT_2884 at 191.005 kb on + strand, within EX28DRAFT_2884 at 191.010 kb on + strand, within EX28DRAFT_2884 at 191.011 kb on - strand, within EX28DRAFT_2884 at 191.179 kb on - strand, within EX28DRAFT_2885 at 191.207 kb on + strand, within EX28DRAFT_2885 at 191.207 kb on + strand, within EX28DRAFT_2885 at 191.207 kb on + strand, within EX28DRAFT_2885 at 191.208 kb on - strand, within EX28DRAFT_2885 at 191.208 kb on - strand, within EX28DRAFT_2885 at 191.275 kb on - strand at 191.285 kb on - strand at 191.357 kb on - strand at 191.382 kb on + strand at 191.409 kb on + strand at 191.409 kb on + strand at 191.410 kb on - strand at 191.452 kb on + strand at 191.453 kb on - strand at 191.453 kb on - strand at 191.454 kb on + strand at 191.537 kb on - strand at 191.537 kb on - strand at 191.600 kb on - strand, within EX28DRAFT_2886 at 191.757 kb on + strand, within EX28DRAFT_2886 at 191.757 kb on + strand, within EX28DRAFT_2886
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 189,867 - -0.4 189,901 + EX28DRAFT_2883 0.13 +0.6 189,902 - EX28DRAFT_2883 0.13 +0.2 189,924 + EX28DRAFT_2883 0.15 +1.0 189,925 - EX28DRAFT_2883 0.15 -1.1 189,925 - EX28DRAFT_2883 0.15 +0.5 189,933 - EX28DRAFT_2883 0.16 -0.3 190,070 + EX28DRAFT_2883 0.31 +1.8 190,070 + EX28DRAFT_2883 0.31 -0.5 190,083 + EX28DRAFT_2883 0.32 +0.5 190,084 - EX28DRAFT_2883 0.32 +0.7 190,182 + EX28DRAFT_2883 0.43 +1.5 190,222 + EX28DRAFT_2883 0.47 -0.7 190,635 - -0.5 190,806 + +0.4 190,806 + +0.2 190,816 + +0.5 190,820 + +1.8 190,820 + +0.4 190,820 + +0.4 190,820 + +0.3 190,820 + +0.7 190,821 - +0.8 190,822 + +0.1 190,983 + EX28DRAFT_2884 0.73 +0.3 190,983 + EX28DRAFT_2884 0.73 -0.3 190,984 - EX28DRAFT_2884 0.73 +2.2 191,005 + EX28DRAFT_2884 0.83 +0.9 191,010 + EX28DRAFT_2884 0.86 -0.3 191,011 - EX28DRAFT_2884 0.86 +1.6 191,179 - EX28DRAFT_2885 0.55 +0.2 191,207 + EX28DRAFT_2885 0.68 -0.3 191,207 + EX28DRAFT_2885 0.68 -0.9 191,207 + EX28DRAFT_2885 0.68 +0.1 191,208 - EX28DRAFT_2885 0.68 -1.1 191,208 - EX28DRAFT_2885 0.68 -0.6 191,275 - -0.1 191,285 - -0.1 191,357 - +0.1 191,382 + -0.3 191,409 + +0.3 191,409 + +0.1 191,410 - -0.7 191,452 + +0.2 191,453 - +0.1 191,453 - +0.4 191,454 + +0.2 191,537 - -0.4 191,537 - -1.2 191,600 - EX28DRAFT_2886 0.11 -0.6 191,757 + EX28DRAFT_2886 0.24 +0.7 191,757 + EX28DRAFT_2886 0.24 +0.3
Or see this region's nucleotide sequence