Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2863 and EX28DRAFT_2864 are separated by 67 nucleotides EX28DRAFT_2864 and EX28DRAFT_2865 are separated by 51 nucleotides
EX28DRAFT_2863: EX28DRAFT_2863 - MazG family protein, at 165,976 to 166,767
_2863
EX28DRAFT_2864: EX28DRAFT_2864 - (p)ppGpp synthetase, RelA/SpoT family, at 166,835 to 169,066
_2864
EX28DRAFT_2865: EX28DRAFT_2865 - 23S rRNA (uracil-5-)-methyltransferase RumA, at 169,118 to 170,416
_2865
Position (kb) 166
167
168
169
170 Strain fitness (log2 ratio) -2
-1
0
1 at 165.874 kb on - strand at 165.902 kb on + strand at 166.330 kb on - strand, within EX28DRAFT_2863 at 166.365 kb on + strand, within EX28DRAFT_2863 at 166.530 kb on + strand, within EX28DRAFT_2863 at 166.562 kb on + strand, within EX28DRAFT_2863 at 166.563 kb on - strand, within EX28DRAFT_2863 at 166.646 kb on + strand, within EX28DRAFT_2863 at 166.663 kb on - strand, within EX28DRAFT_2863 at 166.663 kb on - strand, within EX28DRAFT_2863 at 166.920 kb on + strand at 166.936 kb on + strand at 166.937 kb on - strand at 166.937 kb on - strand at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.111 kb on + strand, within EX28DRAFT_2864 at 167.197 kb on + strand, within EX28DRAFT_2864 at 167.198 kb on - strand, within EX28DRAFT_2864 at 167.317 kb on - strand, within EX28DRAFT_2864 at 168.139 kb on - strand, within EX28DRAFT_2864 at 168.182 kb on + strand, within EX28DRAFT_2864 at 168.183 kb on - strand, within EX28DRAFT_2864 at 168.298 kb on - strand, within EX28DRAFT_2864 at 168.302 kb on - strand, within EX28DRAFT_2864 at 169.080 kb on - strand at 169.120 kb on - strand at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.260 kb on + strand, within EX28DRAFT_2865 at 169.261 kb on - strand, within EX28DRAFT_2865 at 169.285 kb on + strand, within EX28DRAFT_2865 at 169.288 kb on + strand, within EX28DRAFT_2865 at 169.289 kb on - strand, within EX28DRAFT_2865 at 169.289 kb on - strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.291 kb on + strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.292 kb on - strand, within EX28DRAFT_2865 at 169.704 kb on - strand, within EX28DRAFT_2865
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 165,874 - -0.5 165,902 + -0.5 166,330 - EX28DRAFT_2863 0.45 -0.1 166,365 + EX28DRAFT_2863 0.49 -0.4 166,530 + EX28DRAFT_2863 0.70 +0.4 166,562 + EX28DRAFT_2863 0.74 +0.2 166,563 - EX28DRAFT_2863 0.74 +0.2 166,646 + EX28DRAFT_2863 0.85 +0.8 166,663 - EX28DRAFT_2863 0.87 +0.0 166,663 - EX28DRAFT_2863 0.87 +1.2 166,920 + -0.1 166,936 + -1.1 166,937 - -0.3 166,937 - -1.9 167,111 + EX28DRAFT_2864 0.12 -0.6 167,111 + EX28DRAFT_2864 0.12 -0.8 167,111 + EX28DRAFT_2864 0.12 +0.2 167,111 + EX28DRAFT_2864 0.12 -0.9 167,197 + EX28DRAFT_2864 0.16 -1.3 167,198 - EX28DRAFT_2864 0.16 +0.1 167,317 - EX28DRAFT_2864 0.22 +0.1 168,139 - EX28DRAFT_2864 0.58 -1.0 168,182 + EX28DRAFT_2864 0.60 -1.4 168,183 - EX28DRAFT_2864 0.60 +0.2 168,298 - EX28DRAFT_2864 0.66 -2.0 168,302 - EX28DRAFT_2864 0.66 -0.9 169,080 - +0.1 169,120 - -0.0 169,260 + EX28DRAFT_2865 0.11 +0.8 169,260 + EX28DRAFT_2865 0.11 +0.7 169,260 + EX28DRAFT_2865 0.11 -1.7 169,260 + EX28DRAFT_2865 0.11 +0.6 169,261 - EX28DRAFT_2865 0.11 +1.4 169,285 + EX28DRAFT_2865 0.13 -0.4 169,288 + EX28DRAFT_2865 0.13 -0.4 169,289 - EX28DRAFT_2865 0.13 +0.7 169,289 - EX28DRAFT_2865 0.13 +0.0 169,291 + EX28DRAFT_2865 0.13 +0.0 169,291 + EX28DRAFT_2865 0.13 +0.4 169,291 + EX28DRAFT_2865 0.13 -0.8 169,291 + EX28DRAFT_2865 0.13 -1.4 169,291 + EX28DRAFT_2865 0.13 -0.1 169,292 - EX28DRAFT_2865 0.13 -0.0 169,292 - EX28DRAFT_2865 0.13 +0.3 169,292 - EX28DRAFT_2865 0.13 -0.3 169,292 - EX28DRAFT_2865 0.13 -0.7 169,704 - EX28DRAFT_2865 0.45 -1.3
Or see this region's nucleotide sequence