Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2847 and EX28DRAFT_2848 are separated by 9 nucleotides EX28DRAFT_2848 and EX28DRAFT_2849 are separated by 9 nucleotides EX28DRAFT_2849 and EX28DRAFT_2850 are separated by 237 nucleotides
EX28DRAFT_2847: EX28DRAFT_2847 - sulfate adenylyltransferase, large subunit, at 147,145 to 148,569
_2847
EX28DRAFT_2848: EX28DRAFT_2848 - sulfate adenylyltransferase, small subunit, at 148,579 to 149,487
_2848
EX28DRAFT_2849: EX28DRAFT_2849 - uroporphyrin-III C-methyltransferase, at 149,497 to 150,855
_2849
EX28DRAFT_2850: EX28DRAFT_2850 - Predicted aminopeptidases, at 151,093 to 152,136
_2850
Position (kb) 149
150
151 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 148.518 kb on - strand at 148.557 kb on + strand at 148.558 kb on - strand at 148.893 kb on - strand, within EX28DRAFT_2848 at 148.893 kb on - strand, within EX28DRAFT_2848 at 148.976 kb on + strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.218 kb on - strand, within EX28DRAFT_2848 at 149.241 kb on - strand, within EX28DRAFT_2848 at 149.271 kb on - strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.440 kb on - strand at 149.469 kb on + strand at 149.470 kb on - strand at 149.470 kb on - strand at 149.771 kb on + strand, within EX28DRAFT_2849 at 149.771 kb on + strand, within EX28DRAFT_2849 at 149.772 kb on - strand, within EX28DRAFT_2849 at 149.837 kb on + strand, within EX28DRAFT_2849 at 149.838 kb on - strand, within EX28DRAFT_2849 at 150.685 kb on + strand, within EX28DRAFT_2849 at 150.838 kb on + strand at 150.848 kb on + strand at 150.893 kb on - strand at 150.893 kb on - strand at 150.893 kb on - strand at 150.907 kb on - strand at 150.922 kb on - strand at 150.971 kb on - strand at 150.971 kb on - strand at 151.053 kb on + strand at 151.054 kb on - strand at 151.065 kb on + strand at 151.065 kb on + strand at 151.066 kb on - strand at 151.066 kb on - strand at 151.147 kb on - strand at 151.147 kb on - strand at 151.177 kb on + strand at 151.209 kb on + strand, within EX28DRAFT_2850 at 151.210 kb on - strand, within EX28DRAFT_2850 at 151.340 kb on - strand, within EX28DRAFT_2850 at 151.340 kb on - strand, within EX28DRAFT_2850 at 151.341 kb on + strand, within EX28DRAFT_2850 at 151.342 kb on - strand, within EX28DRAFT_2850 at 151.342 kb on - strand, within EX28DRAFT_2850 at 151.371 kb on + strand, within EX28DRAFT_2850 at 151.372 kb on - strand, within EX28DRAFT_2850 at 151.386 kb on + strand, within EX28DRAFT_2850 at 151.431 kb on + strand, within EX28DRAFT_2850 at 151.431 kb on + strand, within EX28DRAFT_2850 at 151.450 kb on - strand, within EX28DRAFT_2850 at 151.454 kb on - strand, within EX28DRAFT_2850 at 151.729 kb on + strand, within EX28DRAFT_2850 at 151.730 kb on - strand, within EX28DRAFT_2850
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 148,518 - -0.9 148,557 + -2.2 148,558 - -2.9 148,893 - EX28DRAFT_2848 0.35 -2.1 148,893 - EX28DRAFT_2848 0.35 -1.9 148,976 + EX28DRAFT_2848 0.44 -4.1 149,047 - EX28DRAFT_2848 0.51 -1.6 149,047 - EX28DRAFT_2848 0.51 -3.9 149,047 - EX28DRAFT_2848 0.51 -3.7 149,047 - EX28DRAFT_2848 0.51 -1.2 149,218 - EX28DRAFT_2848 0.70 -1.5 149,241 - EX28DRAFT_2848 0.73 -3.5 149,271 - EX28DRAFT_2848 0.76 -4.8 149,387 + EX28DRAFT_2848 0.89 -2.7 149,387 + EX28DRAFT_2848 0.89 -1.9 149,387 + EX28DRAFT_2848 0.89 -3.5 149,388 - EX28DRAFT_2848 0.89 -2.4 149,388 - EX28DRAFT_2848 0.89 -1.3 149,388 - EX28DRAFT_2848 0.89 -1.1 149,388 - EX28DRAFT_2848 0.89 -1.7 149,388 - EX28DRAFT_2848 0.89 -2.3 149,440 - -2.4 149,469 + -1.6 149,470 - -2.2 149,470 - +0.4 149,771 + EX28DRAFT_2849 0.20 -2.2 149,771 + EX28DRAFT_2849 0.20 -1.1 149,772 - EX28DRAFT_2849 0.20 -0.9 149,837 + EX28DRAFT_2849 0.25 -1.7 149,838 - EX28DRAFT_2849 0.25 -1.5 150,685 + EX28DRAFT_2849 0.87 -0.9 150,838 + -3.5 150,848 + -3.4 150,893 - +0.5 150,893 - +0.2 150,893 - +0.4 150,907 - -1.5 150,922 - -1.5 150,971 - -0.0 150,971 - -0.2 151,053 + -0.5 151,054 - -0.8 151,065 + +0.1 151,065 + +0.9 151,066 - +0.3 151,066 - -0.1 151,147 - +0.1 151,147 - -0.4 151,177 + -0.1 151,209 + EX28DRAFT_2850 0.11 -0.3 151,210 - EX28DRAFT_2850 0.11 -0.7 151,340 - EX28DRAFT_2850 0.24 -0.4 151,340 - EX28DRAFT_2850 0.24 +0.2 151,341 + EX28DRAFT_2850 0.24 -0.4 151,342 - EX28DRAFT_2850 0.24 -0.6 151,342 - EX28DRAFT_2850 0.24 +0.2 151,371 + EX28DRAFT_2850 0.27 +2.6 151,372 - EX28DRAFT_2850 0.27 -1.3 151,386 + EX28DRAFT_2850 0.28 +0.5 151,431 + EX28DRAFT_2850 0.32 -1.3 151,431 + EX28DRAFT_2850 0.32 +0.1 151,450 - EX28DRAFT_2850 0.34 -0.0 151,454 - EX28DRAFT_2850 0.35 -0.8 151,729 + EX28DRAFT_2850 0.61 +0.1 151,730 - EX28DRAFT_2850 0.61 +0.4
Or see this region's nucleotide sequence