Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2847 and EX28DRAFT_2848 are separated by 9 nucleotides EX28DRAFT_2848 and EX28DRAFT_2849 are separated by 9 nucleotides
EX28DRAFT_2847: EX28DRAFT_2847 - sulfate adenylyltransferase, large subunit, at 147,145 to 148,569
_2847
EX28DRAFT_2848: EX28DRAFT_2848 - sulfate adenylyltransferase, small subunit, at 148,579 to 149,487
_2848
EX28DRAFT_2849: EX28DRAFT_2849 - uroporphyrin-III C-methyltransferase, at 149,497 to 150,855
_2849
Position (kb) 148
149
150 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 147.619 kb on - strand, within EX28DRAFT_2847 at 147.619 kb on - strand, within EX28DRAFT_2847 at 147.748 kb on + strand, within EX28DRAFT_2847 at 147.749 kb on - strand, within EX28DRAFT_2847 at 147.815 kb on + strand, within EX28DRAFT_2847 at 147.816 kb on - strand, within EX28DRAFT_2847 at 147.902 kb on + strand, within EX28DRAFT_2847 at 147.902 kb on + strand, within EX28DRAFT_2847 at 147.902 kb on + strand, within EX28DRAFT_2847 at 147.961 kb on + strand, within EX28DRAFT_2847 at 147.962 kb on - strand, within EX28DRAFT_2847 at 147.972 kb on + strand, within EX28DRAFT_2847 at 148.035 kb on + strand, within EX28DRAFT_2847 at 148.111 kb on - strand, within EX28DRAFT_2847 at 148.111 kb on - strand, within EX28DRAFT_2847 at 148.243 kb on - strand, within EX28DRAFT_2847 at 148.518 kb on - strand at 148.557 kb on + strand at 148.558 kb on - strand at 148.893 kb on - strand, within EX28DRAFT_2848 at 148.893 kb on - strand, within EX28DRAFT_2848 at 148.976 kb on + strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.047 kb on - strand, within EX28DRAFT_2848 at 149.218 kb on - strand, within EX28DRAFT_2848 at 149.241 kb on - strand, within EX28DRAFT_2848 at 149.271 kb on - strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.387 kb on + strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.388 kb on - strand, within EX28DRAFT_2848 at 149.440 kb on - strand at 149.469 kb on + strand at 149.470 kb on - strand at 149.470 kb on - strand at 149.771 kb on + strand, within EX28DRAFT_2849 at 149.771 kb on + strand, within EX28DRAFT_2849 at 149.772 kb on - strand, within EX28DRAFT_2849 at 149.837 kb on + strand, within EX28DRAFT_2849 at 149.838 kb on - strand, within EX28DRAFT_2849
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 147,619 - EX28DRAFT_2847 0.33 -1.0 147,619 - EX28DRAFT_2847 0.33 -1.7 147,748 + EX28DRAFT_2847 0.42 -3.5 147,749 - EX28DRAFT_2847 0.42 -1.9 147,815 + EX28DRAFT_2847 0.47 -2.2 147,816 - EX28DRAFT_2847 0.47 -1.8 147,902 + EX28DRAFT_2847 0.53 -2.1 147,902 + EX28DRAFT_2847 0.53 -2.7 147,902 + EX28DRAFT_2847 0.53 -1.7 147,961 + EX28DRAFT_2847 0.57 -1.5 147,962 - EX28DRAFT_2847 0.57 -1.0 147,972 + EX28DRAFT_2847 0.58 -2.1 148,035 + EX28DRAFT_2847 0.62 -2.5 148,111 - EX28DRAFT_2847 0.68 -1.7 148,111 - EX28DRAFT_2847 0.68 -1.6 148,243 - EX28DRAFT_2847 0.77 -2.9 148,518 - -0.9 148,557 + -2.2 148,558 - -2.9 148,893 - EX28DRAFT_2848 0.35 -2.1 148,893 - EX28DRAFT_2848 0.35 -1.9 148,976 + EX28DRAFT_2848 0.44 -4.1 149,047 - EX28DRAFT_2848 0.51 -1.6 149,047 - EX28DRAFT_2848 0.51 -3.9 149,047 - EX28DRAFT_2848 0.51 -3.7 149,047 - EX28DRAFT_2848 0.51 -1.2 149,218 - EX28DRAFT_2848 0.70 -1.5 149,241 - EX28DRAFT_2848 0.73 -3.5 149,271 - EX28DRAFT_2848 0.76 -4.8 149,387 + EX28DRAFT_2848 0.89 -2.7 149,387 + EX28DRAFT_2848 0.89 -1.9 149,387 + EX28DRAFT_2848 0.89 -3.5 149,388 - EX28DRAFT_2848 0.89 -2.4 149,388 - EX28DRAFT_2848 0.89 -1.3 149,388 - EX28DRAFT_2848 0.89 -1.1 149,388 - EX28DRAFT_2848 0.89 -1.7 149,388 - EX28DRAFT_2848 0.89 -2.3 149,440 - -2.4 149,469 + -1.6 149,470 - -2.2 149,470 - +0.4 149,771 + EX28DRAFT_2849 0.20 -2.2 149,771 + EX28DRAFT_2849 0.20 -1.1 149,772 - EX28DRAFT_2849 0.20 -0.9 149,837 + EX28DRAFT_2849 0.25 -1.7 149,838 - EX28DRAFT_2849 0.25 -1.5
Or see this region's nucleotide sequence