Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_2820

Experiment: M9 with sucrose; no stress control

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_2818 and EX28DRAFT_2819 overlap by 10 nucleotidesEX28DRAFT_2819 and EX28DRAFT_2820 are separated by 2 nucleotidesEX28DRAFT_2820 and EX28DRAFT_2821 overlap by 4 nucleotides EX28DRAFT_2818: EX28DRAFT_2818 - hydrogenase accessory protein HypB, at 121,166 to 122,029 _2818 EX28DRAFT_2819: EX28DRAFT_2819 - hydrogenase assembly chaperone HypC/HupF, at 122,020 to 122,292 _2819 EX28DRAFT_2820: EX28DRAFT_2820 - hydrogenase expression/formation protein HypD, at 122,295 to 123,416 _2820 EX28DRAFT_2821: EX28DRAFT_2821 - hydrogenase expression/formation protein HypE, at 123,413 to 124,423 _2821 Position (kb) 122 123 124Strain fitness (log2 ratio) -2 -1 0 1at 121.837 kb on + strand, within EX28DRAFT_2818at 121.837 kb on + strand, within EX28DRAFT_2818at 121.838 kb on - strand, within EX28DRAFT_2818at 121.889 kb on + strand, within EX28DRAFT_2818at 121.905 kb on + strand, within EX28DRAFT_2818at 121.906 kb on - strand, within EX28DRAFT_2818at 121.909 kb on + strand, within EX28DRAFT_2818at 121.909 kb on + strand, within EX28DRAFT_2818at 121.909 kb on + strand, within EX28DRAFT_2818at 121.910 kb on - strand, within EX28DRAFT_2818at 121.910 kb on - strand, within EX28DRAFT_2818at 121.910 kb on - strand, within EX28DRAFT_2818at 121.910 kb on - strand, within EX28DRAFT_2818at 121.922 kb on + strand, within EX28DRAFT_2818at 121.922 kb on + strand, within EX28DRAFT_2818at 121.923 kb on - strand, within EX28DRAFT_2818at 122.028 kb on - strandat 122.028 kb on - strandat 122.047 kb on + strandat 122.048 kb on - strand, within EX28DRAFT_2819at 122.048 kb on - strand, within EX28DRAFT_2819at 122.193 kb on - strand, within EX28DRAFT_2819at 122.301 kb on + strandat 122.380 kb on - strandat 122.380 kb on - strandat 122.661 kb on + strand, within EX28DRAFT_2820at 122.661 kb on + strand, within EX28DRAFT_2820at 122.662 kb on - strand, within EX28DRAFT_2820at 122.662 kb on - strand, within EX28DRAFT_2820at 122.754 kb on - strand, within EX28DRAFT_2820at 122.823 kb on - strand, within EX28DRAFT_2820at 122.823 kb on - strand, within EX28DRAFT_2820at 122.946 kb on + strand, within EX28DRAFT_2820at 122.947 kb on - strand, within EX28DRAFT_2820at 122.989 kb on + strand, within EX28DRAFT_2820at 122.989 kb on + strand, within EX28DRAFT_2820at 122.989 kb on + strand, within EX28DRAFT_2820at 123.318 kb on - strandat 123.318 kb on - strandat 123.385 kb on - strandat 123.470 kb on - strandat 123.590 kb on + strand, within EX28DRAFT_2821at 123.590 kb on + strand, within EX28DRAFT_2821at 123.591 kb on - strand, within EX28DRAFT_2821at 123.591 kb on - strand, within EX28DRAFT_2821at 123.680 kb on - strand, within EX28DRAFT_2821at 123.690 kb on - strand, within EX28DRAFT_2821at 124.165 kb on + strand, within EX28DRAFT_2821

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control
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121,837 + EX28DRAFT_2818 0.78 +0.1
121,837 + EX28DRAFT_2818 0.78 +0.1
121,838 - EX28DRAFT_2818 0.78 +0.6
121,889 + EX28DRAFT_2818 0.84 -2.1
121,905 + EX28DRAFT_2818 0.86 -0.1
121,906 - EX28DRAFT_2818 0.86 -0.5
121,909 + EX28DRAFT_2818 0.86 -0.4
121,909 + EX28DRAFT_2818 0.86 +0.2
121,909 + EX28DRAFT_2818 0.86 -0.5
121,910 - EX28DRAFT_2818 0.86 +0.0
121,910 - EX28DRAFT_2818 0.86 -0.1
121,910 - EX28DRAFT_2818 0.86 -0.4
121,910 - EX28DRAFT_2818 0.86 +0.9
121,922 + EX28DRAFT_2818 0.88 -0.1
121,922 + EX28DRAFT_2818 0.88 +0.1
121,923 - EX28DRAFT_2818 0.88 -0.1
122,028 - +0.4
122,028 - +0.4
122,047 + -0.2
122,048 - EX28DRAFT_2819 0.10 +0.1
122,048 - EX28DRAFT_2819 0.10 +0.1
122,193 - EX28DRAFT_2819 0.63 -0.7
122,301 + -0.2
122,380 - +0.1
122,380 - -0.5
122,661 + EX28DRAFT_2820 0.33 +0.3
122,661 + EX28DRAFT_2820 0.33 -2.6
122,662 - EX28DRAFT_2820 0.33 -0.4
122,662 - EX28DRAFT_2820 0.33 -0.0
122,754 - EX28DRAFT_2820 0.41 +0.2
122,823 - EX28DRAFT_2820 0.47 +0.6
122,823 - EX28DRAFT_2820 0.47 +0.9
122,946 + EX28DRAFT_2820 0.58 +1.1
122,947 - EX28DRAFT_2820 0.58 +0.8
122,989 + EX28DRAFT_2820 0.62 +0.3
122,989 + EX28DRAFT_2820 0.62 +1.0
122,989 + EX28DRAFT_2820 0.62 -0.3
123,318 - -0.5
123,318 - +1.3
123,385 - -0.5
123,470 - +0.6
123,590 + EX28DRAFT_2821 0.18 -0.2
123,590 + EX28DRAFT_2821 0.18 -0.0
123,591 - EX28DRAFT_2821 0.18 +1.0
123,591 - EX28DRAFT_2821 0.18 -0.1
123,680 - EX28DRAFT_2821 0.26 -1.6
123,690 - EX28DRAFT_2821 0.27 +0.4
124,165 + EX28DRAFT_2821 0.74 +0.0

Or see this region's nucleotide sequence