Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2770 and EX28DRAFT_2771 overlap by 14 nucleotides EX28DRAFT_2771 and EX28DRAFT_2772 are separated by 54 nucleotides EX28DRAFT_2772 and EX28DRAFT_2773 are separated by 72 nucleotides EX28DRAFT_2773 and EX28DRAFT_2774 are separated by 8 nucleotides
EX28DRAFT_2770: EX28DRAFT_2770 - 2-isopropylmalate synthase, yeast type, at 78,763 to 80,418
_2770
EX28DRAFT_2771: EX28DRAFT_2771 - AraC-type DNA-binding domain-containing proteins, at 80,405 to 81,169
_2771
EX28DRAFT_2772: EX28DRAFT_2772 - S-ribosylhomocysteine lyase (EC 4.4.1.21)/quorum-sensing autoinducer 2 (AI-2) synthesis protein LuxS, at 81,224 to 81,739
_2772
EX28DRAFT_2773: EX28DRAFT_2773 - RNA, at 81,812 to 81,887
_2773
EX28DRAFT_2774: EX28DRAFT_2774 - glutamate-cysteine ligase (EC 6.3.2.2), at 81,896 to 83,440
_2774
Position (kb)
81
82 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 80.261 kb on - strand at 80.261 kb on - strand at 80.271 kb on + strand at 80.271 kb on + strand at 80.272 kb on - strand at 80.272 kb on - strand at 80.272 kb on - strand at 80.309 kb on - strand at 80.417 kb on - strand at 80.583 kb on - strand, within EX28DRAFT_2771 at 80.782 kb on - strand, within EX28DRAFT_2771 at 80.782 kb on - strand, within EX28DRAFT_2771 at 80.782 kb on - strand, within EX28DRAFT_2771 at 81.225 kb on + strand at 81.225 kb on + strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.226 kb on - strand at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.348 kb on + strand, within EX28DRAFT_2772 at 81.349 kb on - strand, within EX28DRAFT_2772 at 81.352 kb on + strand, within EX28DRAFT_2772 at 81.363 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.477 kb on + strand, within EX28DRAFT_2772 at 81.577 kb on + strand, within EX28DRAFT_2772 at 81.578 kb on - strand, within EX28DRAFT_2772 at 81.683 kb on + strand, within EX28DRAFT_2772 at 81.725 kb on - strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.728 kb on + strand at 81.732 kb on - strand at 81.739 kb on + strand at 81.740 kb on - strand at 81.774 kb on + strand at 81.789 kb on + strand at 81.790 kb on - strand at 81.808 kb on + strand at 81.809 kb on - strand at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.857 kb on - strand, within EX28DRAFT_2773 at 81.859 kb on - strand, within EX28DRAFT_2773 at 81.898 kb on - strand at 82.090 kb on + strand, within EX28DRAFT_2774 at 82.298 kb on - strand, within EX28DRAFT_2774 at 82.467 kb on - strand, within EX28DRAFT_2774 at 82.476 kb on + strand, within EX28DRAFT_2774 at 82.677 kb on + strand, within EX28DRAFT_2774 at 82.700 kb on - strand, within EX28DRAFT_2774 at 82.724 kb on - strand, within EX28DRAFT_2774
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 80,261 - -0.7 80,261 - -0.5 80,271 + +1.3 80,271 + +1.9 80,272 - +0.1 80,272 - +0.1 80,272 - -0.5 80,309 - -0.2 80,417 - -1.0 80,583 - EX28DRAFT_2771 0.23 +0.3 80,782 - EX28DRAFT_2771 0.49 +0.1 80,782 - EX28DRAFT_2771 0.49 +0.6 80,782 - EX28DRAFT_2771 0.49 -0.3 81,225 + +0.2 81,225 + -0.2 81,226 - +0.3 81,226 - +0.9 81,226 - +1.0 81,348 + EX28DRAFT_2772 0.24 -1.5 81,348 + EX28DRAFT_2772 0.24 +0.3 81,349 - EX28DRAFT_2772 0.24 -2.2 81,352 + EX28DRAFT_2772 0.25 -0.5 81,363 + EX28DRAFT_2772 0.27 -0.5 81,477 + EX28DRAFT_2772 0.49 +0.3 81,477 + EX28DRAFT_2772 0.49 +0.1 81,577 + EX28DRAFT_2772 0.68 -0.3 81,578 - EX28DRAFT_2772 0.69 -0.2 81,683 + EX28DRAFT_2772 0.89 -2.7 81,725 - -0.2 81,728 + +0.7 81,728 + -0.5 81,728 + +0.4 81,728 + +0.3 81,728 + +0.6 81,732 - +0.4 81,739 + -0.2 81,740 - -0.6 81,774 + +0.1 81,789 + +1.8 81,790 - +0.8 81,808 + -1.7 81,809 - +0.0 81,857 - EX28DRAFT_2773 0.59 +3.1 81,857 - EX28DRAFT_2773 0.59 -0.2 81,859 - EX28DRAFT_2773 0.62 +0.1 81,898 - -1.6 82,090 + EX28DRAFT_2774 0.13 -0.3 82,298 - EX28DRAFT_2774 0.26 +2.1 82,467 - EX28DRAFT_2774 0.37 -1.2 82,476 + EX28DRAFT_2774 0.38 -0.9 82,677 + EX28DRAFT_2774 0.51 +0.5 82,700 - EX28DRAFT_2774 0.52 -0.7 82,724 - EX28DRAFT_2774 0.54 +0.3
Or see this region's nucleotide sequence