Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2757 and EX28DRAFT_2758 are separated by 160 nucleotides EX28DRAFT_2758 and EX28DRAFT_2759 are separated by 243 nucleotides EX28DRAFT_2759 and EX28DRAFT_2760 overlap by 4 nucleotides EX28DRAFT_2760 and EX28DRAFT_2761 overlap by 28 nucleotides
EX28DRAFT_2757: EX28DRAFT_2757 - Protein of unknown function (DUF2002), at 65,088 to 65,432
_2757
EX28DRAFT_2758: EX28DRAFT_2758 - Uncharacterized conserved protein, at 65,593 to 65,919
_2758
EX28DRAFT_2759: EX28DRAFT_2759 - ribonucleoside-diphosphate reductase class Ib glutaredoxin subunit (EC 1.17.4.1), at 66,163 to 66,402
_2759
EX28DRAFT_2760: EX28DRAFT_2760 - ribonucleoside-diphosphate reductase 2, operon protein nrdI, at 66,399 to 66,809
_2760
EX28DRAFT_2761: EX28DRAFT_2761 - ribonucleoside-diphosphate reductase, alpha subunit, at 66,782 to 68,926
_2761
Position (kb) 66
67 Strain fitness (log2 ratio) -2
-1
0
1
2 at 65.428 kb on + strand at 65.428 kb on + strand at 65.428 kb on + strand at 65.429 kb on - strand at 65.429 kb on - strand at 65.429 kb on - strand at 65.433 kb on - strand at 65.469 kb on + strand at 65.469 kb on + strand at 65.470 kb on - strand at 65.470 kb on - strand at 65.470 kb on - strand at 65.474 kb on + strand at 65.485 kb on - strand at 65.485 kb on - strand at 65.501 kb on + strand at 65.541 kb on + strand at 65.541 kb on + strand at 65.545 kb on - strand at 65.620 kb on - strand at 65.853 kb on - strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.918 kb on - strand at 66.033 kb on - strand at 66.078 kb on - strand at 66.078 kb on - strand at 66.104 kb on - strand at 66.104 kb on - strand at 66.399 kb on - strand at 66.414 kb on + strand at 66.414 kb on + strand at 66.512 kb on - strand, within EX28DRAFT_2760 at 66.522 kb on + strand, within EX28DRAFT_2760 at 66.543 kb on + strand, within EX28DRAFT_2760 at 66.764 kb on + strand, within EX28DRAFT_2760 at 66.858 kb on - strand at 66.992 kb on + strand at 66.993 kb on - strand at 67.106 kb on + strand, within EX28DRAFT_2761 at 67.107 kb on - strand, within EX28DRAFT_2761 at 67.115 kb on + strand, within EX28DRAFT_2761 at 67.116 kb on - strand, within EX28DRAFT_2761 at 67.118 kb on - strand, within EX28DRAFT_2761 at 67.146 kb on - strand, within EX28DRAFT_2761 at 67.150 kb on - strand, within EX28DRAFT_2761 at 67.327 kb on + strand, within EX28DRAFT_2761 at 67.339 kb on + strand, within EX28DRAFT_2761 at 67.340 kb on - strand, within EX28DRAFT_2761 at 67.340 kb on - strand, within EX28DRAFT_2761 at 67.512 kb on - strand, within EX28DRAFT_2761 at 67.673 kb on - strand, within EX28DRAFT_2761 at 67.673 kb on - strand, within EX28DRAFT_2761 at 67.688 kb on - strand, within EX28DRAFT_2761 at 67.729 kb on + strand, within EX28DRAFT_2761
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 65,428 + +0.3 65,428 + +0.2 65,428 + +0.1 65,429 - +0.1 65,429 - +1.1 65,429 - +1.2 65,433 - +0.1 65,469 + +0.3 65,469 + -0.0 65,470 - +0.8 65,470 - -0.4 65,470 - -1.9 65,474 + -0.1 65,485 - -0.2 65,485 - -0.3 65,501 + +0.6 65,541 + -0.8 65,541 + +0.9 65,545 - +0.2 65,620 - -0.1 65,853 - EX28DRAFT_2758 0.80 +0.1 65,856 + EX28DRAFT_2758 0.80 -1.8 65,856 + EX28DRAFT_2758 0.80 -2.2 65,856 + EX28DRAFT_2758 0.80 -1.5 65,857 - EX28DRAFT_2758 0.81 +0.7 65,857 - EX28DRAFT_2758 0.81 +0.4 65,918 - -0.1 66,033 - +0.5 66,078 - -0.9 66,078 - -0.3 66,104 - +0.6 66,104 - -0.3 66,399 - +0.4 66,414 + -1.8 66,414 + -0.4 66,512 - EX28DRAFT_2760 0.27 +1.9 66,522 + EX28DRAFT_2760 0.30 +0.3 66,543 + EX28DRAFT_2760 0.35 +0.2 66,764 + EX28DRAFT_2760 0.89 +0.5 66,858 - +0.3 66,992 + +0.4 66,993 - +0.4 67,106 + EX28DRAFT_2761 0.15 -0.7 67,107 - EX28DRAFT_2761 0.15 -1.3 67,115 + EX28DRAFT_2761 0.16 +0.4 67,116 - EX28DRAFT_2761 0.16 -0.8 67,118 - EX28DRAFT_2761 0.16 +0.3 67,146 - EX28DRAFT_2761 0.17 -0.0 67,150 - EX28DRAFT_2761 0.17 -0.6 67,327 + EX28DRAFT_2761 0.25 +0.5 67,339 + EX28DRAFT_2761 0.26 +0.4 67,340 - EX28DRAFT_2761 0.26 -0.2 67,340 - EX28DRAFT_2761 0.26 -0.1 67,512 - EX28DRAFT_2761 0.34 -0.3 67,673 - EX28DRAFT_2761 0.42 +0.2 67,673 - EX28DRAFT_2761 0.42 -1.6 67,688 - EX28DRAFT_2761 0.42 +1.1 67,729 + EX28DRAFT_2761 0.44 -0.7
Or see this region's nucleotide sequence