Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2734 and EX28DRAFT_2735 are separated by 187 nucleotides EX28DRAFT_2735 and EX28DRAFT_2736 are separated by 17 nucleotides EX28DRAFT_2736 and EX28DRAFT_2737 are separated by 88 nucleotides EX28DRAFT_2737 and EX28DRAFT_2738 are separated by 57 nucleotides EX28DRAFT_2738 and EX28DRAFT_2739 are separated by 2 nucleotides
EX28DRAFT_2734: EX28DRAFT_2734 - Multidrug resistance efflux pump, at 46,471 to 47,670
_2734
EX28DRAFT_2735: EX28DRAFT_2735 - RNA, at 47,858 to 47,945
_2735
EX28DRAFT_2736: EX28DRAFT_2736 - hypothetical protein, at 47,963 to 48,341
_2736
EX28DRAFT_2737: EX28DRAFT_2737 - addiction module antidote protein, HigA family, at 48,430 to 48,699
_2737
EX28DRAFT_2738: EX28DRAFT_2738 - ADP-ribose pyrophosphatase, at 48,757 to 49,182
_2738
EX28DRAFT_2739: EX28DRAFT_2739 - hypothetical protein, at 49,185 to 49,694
_2739
Position (kb) 47
48
49 Strain fitness (log2 ratio) -2
-1
0
1
2 at 46.979 kb on + strand, within EX28DRAFT_2734 at 46.980 kb on - strand, within EX28DRAFT_2734 at 46.980 kb on - strand, within EX28DRAFT_2734 at 47.102 kb on + strand, within EX28DRAFT_2734 at 47.102 kb on + strand, within EX28DRAFT_2734 at 47.103 kb on - strand, within EX28DRAFT_2734 at 47.117 kb on - strand, within EX28DRAFT_2734 at 47.117 kb on - strand, within EX28DRAFT_2734 at 47.163 kb on + strand, within EX28DRAFT_2734 at 47.465 kb on + strand, within EX28DRAFT_2734 at 47.494 kb on + strand, within EX28DRAFT_2734 at 47.494 kb on + strand, within EX28DRAFT_2734 at 47.494 kb on + strand, within EX28DRAFT_2734 at 47.495 kb on - strand, within EX28DRAFT_2734 at 47.528 kb on - strand, within EX28DRAFT_2734 at 47.528 kb on - strand, within EX28DRAFT_2734 at 47.538 kb on + strand, within EX28DRAFT_2734 at 47.592 kb on + strand at 47.593 kb on - strand at 47.681 kb on + strand at 47.681 kb on + strand at 47.682 kb on - strand at 47.682 kb on - strand at 47.682 kb on - strand at 47.682 kb on - strand at 47.682 kb on - strand at 47.682 kb on - strand at 47.743 kb on - strand at 47.758 kb on + strand at 47.759 kb on - strand at 48.289 kb on + strand, within EX28DRAFT_2736 at 48.289 kb on + strand, within EX28DRAFT_2736 at 48.569 kb on + strand, within EX28DRAFT_2737 at 48.577 kb on - strand, within EX28DRAFT_2737 at 48.702 kb on + strand at 48.702 kb on + strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.703 kb on - strand at 48.723 kb on - strand at 48.723 kb on - strand at 48.727 kb on + strand at 48.997 kb on + strand, within EX28DRAFT_2738 at 49.025 kb on - strand, within EX28DRAFT_2738 at 49.033 kb on + strand, within EX28DRAFT_2738 at 49.034 kb on - strand, within EX28DRAFT_2738 at 49.122 kb on - strand, within EX28DRAFT_2738 at 49.152 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.207 kb on + strand at 49.208 kb on - strand at 49.208 kb on - strand at 49.208 kb on - strand at 49.208 kb on - strand at 49.208 kb on - strand at 49.339 kb on + strand, within EX28DRAFT_2739 at 49.339 kb on + strand, within EX28DRAFT_2739 at 49.340 kb on - strand, within EX28DRAFT_2739 at 49.340 kb on - strand, within EX28DRAFT_2739
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 46,979 + EX28DRAFT_2734 0.42 +2.2 46,980 - EX28DRAFT_2734 0.42 +0.2 46,980 - EX28DRAFT_2734 0.42 -1.0 47,102 + EX28DRAFT_2734 0.53 +0.3 47,102 + EX28DRAFT_2734 0.53 -0.3 47,103 - EX28DRAFT_2734 0.53 +0.1 47,117 - EX28DRAFT_2734 0.54 -0.1 47,117 - EX28DRAFT_2734 0.54 +0.5 47,163 + EX28DRAFT_2734 0.58 +0.3 47,465 + EX28DRAFT_2734 0.83 -1.9 47,494 + EX28DRAFT_2734 0.85 +0.3 47,494 + EX28DRAFT_2734 0.85 -0.7 47,494 + EX28DRAFT_2734 0.85 +0.8 47,495 - EX28DRAFT_2734 0.85 -0.3 47,528 - EX28DRAFT_2734 0.88 -0.2 47,528 - EX28DRAFT_2734 0.88 -0.2 47,538 + EX28DRAFT_2734 0.89 +0.8 47,592 + +0.4 47,593 - +0.7 47,681 + -0.4 47,681 + +0.2 47,682 - +0.8 47,682 - +0.0 47,682 - -0.2 47,682 - +0.5 47,682 - +1.0 47,682 - +0.8 47,743 - +0.5 47,758 + -0.4 47,759 - -0.2 48,289 + EX28DRAFT_2736 0.86 -0.0 48,289 + EX28DRAFT_2736 0.86 +1.5 48,569 + EX28DRAFT_2737 0.51 +0.5 48,577 - EX28DRAFT_2737 0.54 +0.5 48,702 + +0.3 48,702 + +0.5 48,703 - -0.5 48,703 - +0.1 48,703 - +0.1 48,703 - +0.5 48,703 - -0.1 48,703 - -0.3 48,703 - +0.3 48,703 - -0.3 48,703 - +0.7 48,723 - +0.7 48,723 - -0.1 48,727 + -0.1 48,997 + EX28DRAFT_2738 0.56 -0.9 49,025 - EX28DRAFT_2738 0.63 +0.9 49,033 + EX28DRAFT_2738 0.65 +0.1 49,034 - EX28DRAFT_2738 0.65 -0.2 49,122 - EX28DRAFT_2738 0.86 +0.6 49,152 + +1.2 49,207 + -1.0 49,207 + +0.3 49,207 + -0.2 49,207 + +0.3 49,207 + +0.2 49,208 - +0.6 49,208 - -0.2 49,208 - -0.4 49,208 - +1.3 49,208 - +0.5 49,339 + EX28DRAFT_2739 0.30 -0.2 49,339 + EX28DRAFT_2739 0.30 -0.0 49,340 - EX28DRAFT_2739 0.30 -0.0 49,340 - EX28DRAFT_2739 0.30 -0.7
Or see this region's nucleotide sequence