Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2724 and EX28DRAFT_2725 are separated by 416 nucleotides EX28DRAFT_2725 and EX28DRAFT_2726 are separated by 87 nucleotides EX28DRAFT_2726 and EX28DRAFT_2727 are separated by 68 nucleotides EX28DRAFT_2727 and EX28DRAFT_2728 overlap by 9 nucleotides
EX28DRAFT_2724: EX28DRAFT_2724 - RNA, at 27,044 to 27,407
_2724
EX28DRAFT_2725: EX28DRAFT_2725 - hypothetical protein, at 27,824 to 28,180
_2725
EX28DRAFT_2726: EX28DRAFT_2726 - Cupin domain, at 28,268 to 28,612
_2726
EX28DRAFT_2727: EX28DRAFT_2727 - Uncharacterized conserved protein, at 28,681 to 29,238
_2727
EX28DRAFT_2728: EX28DRAFT_2728 - hypothetical protein, at 29,230 to 30,021
_2728
Position (kb) 28
29 Strain fitness (log2 ratio) -2
-1
0
1
2 at 27.344 kb on + strand, within EX28DRAFT_2724 at 27.344 kb on + strand, within EX28DRAFT_2724 at 27.618 kb on + strand at 27.618 kb on + strand at 27.619 kb on - strand at 27.619 kb on - strand at 27.638 kb on + strand at 27.638 kb on + strand at 27.638 kb on + strand at 27.639 kb on - strand at 27.639 kb on - strand at 27.729 kb on + strand at 27.810 kb on + strand at 27.811 kb on - strand at 27.821 kb on - strand at 27.823 kb on + strand at 27.824 kb on - strand at 27.838 kb on + strand at 27.839 kb on - strand at 27.846 kb on + strand at 27.847 kb on - strand at 28.012 kb on + strand, within EX28DRAFT_2725 at 28.178 kb on + strand at 28.243 kb on - strand at 28.243 kb on - strand at 28.243 kb on - strand at 28.317 kb on + strand, within EX28DRAFT_2726 at 28.317 kb on + strand, within EX28DRAFT_2726 at 28.318 kb on - strand, within EX28DRAFT_2726 at 28.430 kb on - strand, within EX28DRAFT_2726 at 28.617 kb on + strand at 28.665 kb on + strand at 28.697 kb on + strand at 28.698 kb on - strand at 28.701 kb on - strand at 28.701 kb on - strand at 28.748 kb on - strand, within EX28DRAFT_2727 at 28.748 kb on - strand, within EX28DRAFT_2727 at 28.751 kb on + strand, within EX28DRAFT_2727 at 28.752 kb on - strand, within EX28DRAFT_2727 at 28.752 kb on - strand, within EX28DRAFT_2727 at 28.752 kb on - strand, within EX28DRAFT_2727 at 28.910 kb on + strand, within EX28DRAFT_2727 at 29.163 kb on + strand, within EX28DRAFT_2727 at 29.233 kb on + strand at 29.251 kb on + strand at 29.251 kb on + strand at 29.312 kb on + strand, within EX28DRAFT_2728 at 29.389 kb on + strand, within EX28DRAFT_2728 at 29.400 kb on + strand, within EX28DRAFT_2728 at 29.401 kb on - strand, within EX28DRAFT_2728 at 29.401 kb on - strand, within EX28DRAFT_2728 at 29.465 kb on + strand, within EX28DRAFT_2728
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 27,344 + EX28DRAFT_2724 0.82 -1.9 27,344 + EX28DRAFT_2724 0.82 +0.6 27,618 + +0.6 27,618 + -0.2 27,619 - -2.2 27,619 - +1.2 27,638 + -0.3 27,638 + +0.0 27,638 + +0.1 27,639 - -0.0 27,639 - +0.4 27,729 + +0.5 27,810 + +0.2 27,811 - +1.0 27,821 - +0.1 27,823 + +0.2 27,824 - -0.8 27,838 + +2.0 27,839 - +0.9 27,846 + -0.4 27,847 - +0.1 28,012 + EX28DRAFT_2725 0.53 +0.3 28,178 + +0.1 28,243 - -0.0 28,243 - -1.2 28,243 - +0.6 28,317 + EX28DRAFT_2726 0.14 +0.0 28,317 + EX28DRAFT_2726 0.14 +0.6 28,318 - EX28DRAFT_2726 0.14 +0.7 28,430 - EX28DRAFT_2726 0.47 +0.7 28,617 + -1.6 28,665 + +0.9 28,697 + +0.1 28,698 - -1.7 28,701 - +0.4 28,701 - +0.4 28,748 - EX28DRAFT_2727 0.12 -0.6 28,748 - EX28DRAFT_2727 0.12 -0.2 28,751 + EX28DRAFT_2727 0.13 -0.1 28,752 - EX28DRAFT_2727 0.13 +0.1 28,752 - EX28DRAFT_2727 0.13 +0.3 28,752 - EX28DRAFT_2727 0.13 +0.3 28,910 + EX28DRAFT_2727 0.41 +0.1 29,163 + EX28DRAFT_2727 0.86 -0.0 29,233 + +0.9 29,251 + +0.4 29,251 + +0.1 29,312 + EX28DRAFT_2728 0.10 +0.3 29,389 + EX28DRAFT_2728 0.20 -1.9 29,400 + EX28DRAFT_2728 0.21 -0.8 29,401 - EX28DRAFT_2728 0.22 +0.5 29,401 - EX28DRAFT_2728 0.22 -0.1 29,465 + EX28DRAFT_2728 0.30 +0.7
Or see this region's nucleotide sequence