Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2638 and EX28DRAFT_2639 are separated by 71 nucleotides EX28DRAFT_2639 and EX28DRAFT_2640 overlap by 8 nucleotides EX28DRAFT_2640 and EX28DRAFT_2641 overlap by 8 nucleotides EX28DRAFT_2641 and EX28DRAFT_2642 are separated by 215 nucleotides
EX28DRAFT_2638: EX28DRAFT_2638 - hypothetical protein, at 2,678,999 to 2,679,259
_2638
EX28DRAFT_2639: EX28DRAFT_2639 - hypothetical protein, at 2,679,331 to 2,679,780
_2639
EX28DRAFT_2640: EX28DRAFT_2640 - hypothetical protein, at 2,679,773 to 2,680,054
_2640
EX28DRAFT_2641: EX28DRAFT_2641 - Ash protein family, at 2,680,047 to 2,680,382
_2641
EX28DRAFT_2642: EX28DRAFT_2642 - transcriptional regulator, AlpA family, at 2,680,598 to 2,680,864
_2642
Position (kb)
2680
2681 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2679.076 kb on - strand, within EX28DRAFT_2638 at 2679.122 kb on - strand, within EX28DRAFT_2638 at 2679.233 kb on + strand, within EX28DRAFT_2638 at 2679.233 kb on + strand, within EX28DRAFT_2638 at 2679.299 kb on - strand at 2679.478 kb on + strand, within EX28DRAFT_2639 at 2679.478 kb on + strand, within EX28DRAFT_2639 at 2679.479 kb on - strand, within EX28DRAFT_2639 at 2679.537 kb on + strand, within EX28DRAFT_2639 at 2679.542 kb on - strand, within EX28DRAFT_2639 at 2679.561 kb on + strand, within EX28DRAFT_2639 at 2679.561 kb on + strand, within EX28DRAFT_2639 at 2679.562 kb on - strand, within EX28DRAFT_2639 at 2679.562 kb on - strand, within EX28DRAFT_2639 at 2679.616 kb on - strand, within EX28DRAFT_2639 at 2679.635 kb on + strand, within EX28DRAFT_2639 at 2679.635 kb on + strand, within EX28DRAFT_2639 at 2679.750 kb on - strand at 2679.835 kb on + strand, within EX28DRAFT_2640 at 2679.858 kb on + strand, within EX28DRAFT_2640 at 2679.859 kb on - strand, within EX28DRAFT_2640 at 2679.859 kb on - strand, within EX28DRAFT_2640 at 2679.880 kb on - strand, within EX28DRAFT_2640 at 2679.880 kb on - strand, within EX28DRAFT_2640 at 2679.880 kb on - strand, within EX28DRAFT_2640 at 2679.889 kb on + strand, within EX28DRAFT_2640 at 2680.048 kb on + strand at 2680.048 kb on + strand at 2680.048 kb on + strand at 2680.099 kb on + strand, within EX28DRAFT_2641 at 2680.099 kb on + strand, within EX28DRAFT_2641 at 2680.209 kb on - strand, within EX28DRAFT_2641 at 2680.383 kb on + strand at 2680.384 kb on - strand at 2680.384 kb on - strand at 2680.404 kb on + strand at 2680.404 kb on + strand at 2680.404 kb on + strand at 2680.425 kb on + strand at 2680.426 kb on - strand at 2680.440 kb on + strand at 2680.441 kb on - strand at 2680.448 kb on - strand at 2680.488 kb on - strand at 2680.488 kb on - strand at 2680.579 kb on - strand at 2680.817 kb on + strand, within EX28DRAFT_2642 at 2680.818 kb on - strand, within EX28DRAFT_2642 at 2680.818 kb on - strand, within EX28DRAFT_2642 at 2680.818 kb on - strand, within EX28DRAFT_2642 at 2680.850 kb on + strand at 2680.865 kb on - strand at 2680.902 kb on - strand at 2680.995 kb on + strand at 2680.996 kb on - strand at 2680.996 kb on - strand at 2680.997 kb on + strand at 2680.997 kb on + strand at 2680.997 kb on + strand at 2680.997 kb on + strand at 2680.997 kb on + strand at 2680.998 kb on - strand at 2680.998 kb on - strand at 2681.000 kb on - strand at 2681.003 kb on + strand at 2681.004 kb on - strand at 2681.004 kb on - strand at 2681.004 kb on - strand at 2681.009 kb on + strand at 2681.009 kb on + strand at 2681.014 kb on + strand at 2681.086 kb on + strand at 2681.087 kb on - strand at 2681.099 kb on + strand at 2681.239 kb on - strand at 2681.245 kb on + strand at 2681.251 kb on + strand at 2681.251 kb on + strand at 2681.251 kb on + strand at 2681.252 kb on - strand at 2681.252 kb on - strand at 2681.252 kb on - strand at 2681.350 kb on + strand at 2681.368 kb on + strand at 2681.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,679,076 - EX28DRAFT_2638 0.30 +1.0 2,679,122 - EX28DRAFT_2638 0.47 +0.4 2,679,233 + EX28DRAFT_2638 0.90 +0.3 2,679,233 + EX28DRAFT_2638 0.90 +0.8 2,679,299 - +1.3 2,679,478 + EX28DRAFT_2639 0.33 +0.1 2,679,478 + EX28DRAFT_2639 0.33 +0.2 2,679,479 - EX28DRAFT_2639 0.33 -0.4 2,679,537 + EX28DRAFT_2639 0.46 +1.2 2,679,542 - EX28DRAFT_2639 0.47 +1.0 2,679,561 + EX28DRAFT_2639 0.51 -0.3 2,679,561 + EX28DRAFT_2639 0.51 -0.4 2,679,562 - EX28DRAFT_2639 0.51 -5.1 2,679,562 - EX28DRAFT_2639 0.51 -0.4 2,679,616 - EX28DRAFT_2639 0.63 -0.5 2,679,635 + EX28DRAFT_2639 0.68 -0.4 2,679,635 + EX28DRAFT_2639 0.68 -0.2 2,679,750 - +1.2 2,679,835 + EX28DRAFT_2640 0.22 +0.0 2,679,858 + EX28DRAFT_2640 0.30 -0.3 2,679,859 - EX28DRAFT_2640 0.30 +0.3 2,679,859 - EX28DRAFT_2640 0.30 -0.8 2,679,880 - EX28DRAFT_2640 0.38 -0.8 2,679,880 - EX28DRAFT_2640 0.38 +0.0 2,679,880 - EX28DRAFT_2640 0.38 +0.1 2,679,889 + EX28DRAFT_2640 0.41 -0.5 2,680,048 + +0.5 2,680,048 + +0.5 2,680,048 + +0.2 2,680,099 + EX28DRAFT_2641 0.15 -0.6 2,680,099 + EX28DRAFT_2641 0.15 -1.8 2,680,209 - EX28DRAFT_2641 0.48 -0.6 2,680,383 + -0.9 2,680,384 - +1.3 2,680,384 - -1.3 2,680,404 + +0.2 2,680,404 + +0.3 2,680,404 + -0.7 2,680,425 + +0.5 2,680,426 - -0.2 2,680,440 + +0.8 2,680,441 - +0.3 2,680,448 - +0.2 2,680,488 - -0.4 2,680,488 - -0.3 2,680,579 - +0.0 2,680,817 + EX28DRAFT_2642 0.82 +0.3 2,680,818 - EX28DRAFT_2642 0.82 +0.5 2,680,818 - EX28DRAFT_2642 0.82 +0.5 2,680,818 - EX28DRAFT_2642 0.82 +0.2 2,680,850 + -0.1 2,680,865 - +0.7 2,680,902 - +0.7 2,680,995 + +2.0 2,680,996 - +0.2 2,680,996 - -1.1 2,680,997 + +0.2 2,680,997 + -1.2 2,680,997 + +0.0 2,680,997 + -0.5 2,680,997 + -0.3 2,680,998 - +0.7 2,680,998 - -0.1 2,681,000 - +0.0 2,681,003 + -0.2 2,681,004 - -0.5 2,681,004 - -0.1 2,681,004 - -0.0 2,681,009 + -0.6 2,681,009 + -0.4 2,681,014 + +0.7 2,681,086 + -0.3 2,681,087 - -0.7 2,681,099 + -0.3 2,681,239 - +0.8 2,681,245 + -0.8 2,681,251 + +0.0 2,681,251 + -0.5 2,681,251 + -0.2 2,681,252 - -0.2 2,681,252 - +0.6 2,681,252 - -0.1 2,681,350 + +0.3 2,681,368 + -0.5 2,681,368 + -0.5
Or see this region's nucleotide sequence