Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2380 and EX28DRAFT_2381 overlap by 4 nucleotides EX28DRAFT_2381 and EX28DRAFT_2382 are separated by 10 nucleotides EX28DRAFT_2382 and EX28DRAFT_2383 are separated by 68 nucleotides
EX28DRAFT_2380: EX28DRAFT_2380 - amino acid adenylation domain, at 2,400,788 to 2,404,645
_2380
EX28DRAFT_2381: EX28DRAFT_2381 - Uncharacterized protein conserved in bacteria, at 2,404,642 to 2,404,854
_2381
EX28DRAFT_2382: EX28DRAFT_2382 - Enterochelin esterase and related enzymes, at 2,404,865 to 2,406,139
_2382
EX28DRAFT_2383: EX28DRAFT_2383 - TonB-dependent siderophore receptor, at 2,406,208 to 2,408,448
_2383
Position (kb)
2404
2405
2406
2407 Strain fitness (log2 ratio)
-2
-1
0
1 at 2404.092 kb on + strand, within EX28DRAFT_2380 at 2404.119 kb on + strand, within EX28DRAFT_2380 at 2404.419 kb on + strand at 2404.420 kb on - strand at 2404.420 kb on - strand at 2404.554 kb on + strand at 2404.555 kb on - strand at 2404.976 kb on + strand at 2404.977 kb on - strand at 2404.986 kb on + strand at 2405.131 kb on - strand, within EX28DRAFT_2382 at 2405.267 kb on + strand, within EX28DRAFT_2382 at 2405.267 kb on + strand, within EX28DRAFT_2382 at 2405.268 kb on - strand, within EX28DRAFT_2382 at 2405.268 kb on - strand, within EX28DRAFT_2382 at 2405.342 kb on + strand, within EX28DRAFT_2382 at 2405.342 kb on + strand, within EX28DRAFT_2382 at 2405.342 kb on + strand, within EX28DRAFT_2382 at 2405.363 kb on + strand, within EX28DRAFT_2382 at 2405.364 kb on - strand, within EX28DRAFT_2382 at 2405.367 kb on + strand, within EX28DRAFT_2382 at 2405.368 kb on - strand, within EX28DRAFT_2382 at 2405.368 kb on - strand, within EX28DRAFT_2382 at 2405.775 kb on - strand, within EX28DRAFT_2382 at 2406.065 kb on + strand at 2406.065 kb on + strand at 2406.131 kb on + strand at 2406.132 kb on - strand at 2406.151 kb on + strand at 2406.182 kb on - strand at 2406.191 kb on + strand at 2406.204 kb on + strand at 2406.205 kb on - strand at 2406.213 kb on + strand at 2406.257 kb on - strand at 2406.257 kb on - strand at 2406.348 kb on - strand at 2406.567 kb on + strand, within EX28DRAFT_2383 at 2406.757 kb on + strand, within EX28DRAFT_2383 at 2406.758 kb on - strand, within EX28DRAFT_2383 at 2406.777 kb on + strand, within EX28DRAFT_2383 at 2407.111 kb on + strand, within EX28DRAFT_2383 at 2407.131 kb on + strand, within EX28DRAFT_2383 at 2407.131 kb on + strand, within EX28DRAFT_2383 at 2407.132 kb on - strand, within EX28DRAFT_2383
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,404,092 + EX28DRAFT_2380 0.86 +0.1 2,404,119 + EX28DRAFT_2380 0.86 -0.4 2,404,419 + -0.1 2,404,420 - +0.1 2,404,420 - -0.7 2,404,554 + +0.2 2,404,555 - -0.2 2,404,976 + -0.7 2,404,977 - -1.4 2,404,986 + -2.4 2,405,131 - EX28DRAFT_2382 0.21 +0.7 2,405,267 + EX28DRAFT_2382 0.32 +0.7 2,405,267 + EX28DRAFT_2382 0.32 -0.5 2,405,268 - EX28DRAFT_2382 0.32 -0.7 2,405,268 - EX28DRAFT_2382 0.32 -1.8 2,405,342 + EX28DRAFT_2382 0.37 -1.9 2,405,342 + EX28DRAFT_2382 0.37 -0.8 2,405,342 + EX28DRAFT_2382 0.37 -1.4 2,405,363 + EX28DRAFT_2382 0.39 +0.1 2,405,364 - EX28DRAFT_2382 0.39 -1.1 2,405,367 + EX28DRAFT_2382 0.39 -0.5 2,405,368 - EX28DRAFT_2382 0.39 +0.3 2,405,368 - EX28DRAFT_2382 0.39 +0.4 2,405,775 - EX28DRAFT_2382 0.71 +1.2 2,406,065 + -0.7 2,406,065 + -2.5 2,406,131 + +1.3 2,406,132 - +0.0 2,406,151 + -0.5 2,406,182 - -1.0 2,406,191 + -0.6 2,406,204 + -1.0 2,406,205 - -2.3 2,406,213 + -0.2 2,406,257 - +0.0 2,406,257 - -0.6 2,406,348 - -0.4 2,406,567 + EX28DRAFT_2383 0.16 -0.9 2,406,757 + EX28DRAFT_2383 0.24 -1.4 2,406,758 - EX28DRAFT_2383 0.25 -0.3 2,406,777 + EX28DRAFT_2383 0.25 +0.0 2,407,111 + EX28DRAFT_2383 0.40 -1.3 2,407,131 + EX28DRAFT_2383 0.41 -1.5 2,407,131 + EX28DRAFT_2383 0.41 -0.5 2,407,132 - EX28DRAFT_2383 0.41 -0.6
Or see this region's nucleotide sequence