Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2351 and EX28DRAFT_2352 are separated by 117 nucleotides EX28DRAFT_2352 and EX28DRAFT_2353 are separated by 0 nucleotides EX28DRAFT_2353 and EX28DRAFT_2354 are separated by 122 nucleotides EX28DRAFT_2354 and EX28DRAFT_2355 overlap by 4 nucleotides
EX28DRAFT_2351: EX28DRAFT_2351 - Transcriptional regulator, at 2,372,914 to 2,373,813
_2351
EX28DRAFT_2352: EX28DRAFT_2352 - hypothetical protein, at 2,373,931 to 2,374,083
_2352
EX28DRAFT_2353: EX28DRAFT_2353 - Aspartate/tyrosine/aromatic aminotransferase, at 2,374,084 to 2,375,244
_2353
EX28DRAFT_2354: EX28DRAFT_2354 - methylthioribulose-1-phosphate dehydratase (EC 4.2.1.109), at 2,375,367 to 2,375,981
_2354
EX28DRAFT_2355: EX28DRAFT_2355 - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, at 2,375,978 to 2,376,667
_2355
Position (kb)
2374
2375
2376 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2373.097 kb on - strand, within EX28DRAFT_2351 at 2373.181 kb on - strand, within EX28DRAFT_2351 at 2373.181 kb on - strand, within EX28DRAFT_2351 at 2373.235 kb on - strand, within EX28DRAFT_2351 at 2373.369 kb on - strand, within EX28DRAFT_2351 at 2373.388 kb on - strand, within EX28DRAFT_2351 at 2373.420 kb on + strand, within EX28DRAFT_2351 at 2373.421 kb on - strand, within EX28DRAFT_2351 at 2373.421 kb on - strand, within EX28DRAFT_2351 at 2373.583 kb on - strand, within EX28DRAFT_2351 at 2373.594 kb on + strand, within EX28DRAFT_2351 at 2373.607 kb on - strand, within EX28DRAFT_2351 at 2373.735 kb on + strand at 2373.740 kb on - strand at 2373.749 kb on - strand at 2373.890 kb on - strand at 2373.899 kb on + strand at 2373.900 kb on - strand at 2373.930 kb on + strand at 2373.949 kb on + strand, within EX28DRAFT_2352 at 2373.950 kb on - strand, within EX28DRAFT_2352 at 2373.953 kb on + strand, within EX28DRAFT_2352 at 2374.081 kb on + strand at 2374.159 kb on - strand at 2374.287 kb on - strand, within EX28DRAFT_2353 at 2374.287 kb on - strand, within EX28DRAFT_2353 at 2374.361 kb on + strand, within EX28DRAFT_2353 at 2374.362 kb on - strand, within EX28DRAFT_2353 at 2374.532 kb on + strand, within EX28DRAFT_2353 at 2374.532 kb on + strand, within EX28DRAFT_2353 at 2374.653 kb on - strand, within EX28DRAFT_2353 at 2374.862 kb on + strand, within EX28DRAFT_2353 at 2374.863 kb on - strand, within EX28DRAFT_2353 at 2374.871 kb on + strand, within EX28DRAFT_2353 at 2374.932 kb on - strand, within EX28DRAFT_2353 at 2374.962 kb on + strand, within EX28DRAFT_2353 at 2374.963 kb on - strand, within EX28DRAFT_2353 at 2375.085 kb on - strand, within EX28DRAFT_2353 at 2375.101 kb on + strand, within EX28DRAFT_2353 at 2375.101 kb on + strand, within EX28DRAFT_2353 at 2375.102 kb on - strand, within EX28DRAFT_2353 at 2375.102 kb on - strand, within EX28DRAFT_2353 at 2375.102 kb on - strand, within EX28DRAFT_2353 at 2375.193 kb on + strand at 2375.262 kb on + strand at 2375.269 kb on + strand at 2375.269 kb on + strand at 2375.270 kb on - strand at 2375.319 kb on + strand at 2375.334 kb on + strand at 2375.334 kb on + strand at 2375.453 kb on + strand, within EX28DRAFT_2354 at 2375.610 kb on - strand, within EX28DRAFT_2354 at 2375.610 kb on - strand, within EX28DRAFT_2354 at 2375.846 kb on + strand, within EX28DRAFT_2354 at 2375.846 kb on + strand, within EX28DRAFT_2354 at 2375.847 kb on - strand, within EX28DRAFT_2354 at 2375.878 kb on + strand, within EX28DRAFT_2354 at 2375.929 kb on + strand at 2375.930 kb on - strand at 2375.939 kb on - strand at 2376.193 kb on + strand, within EX28DRAFT_2355
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,373,097 - EX28DRAFT_2351 0.20 -0.1 2,373,181 - EX28DRAFT_2351 0.30 -0.3 2,373,181 - EX28DRAFT_2351 0.30 -0.1 2,373,235 - EX28DRAFT_2351 0.36 +0.4 2,373,369 - EX28DRAFT_2351 0.51 -0.3 2,373,388 - EX28DRAFT_2351 0.53 -0.6 2,373,420 + EX28DRAFT_2351 0.56 +0.3 2,373,421 - EX28DRAFT_2351 0.56 +0.3 2,373,421 - EX28DRAFT_2351 0.56 +1.3 2,373,583 - EX28DRAFT_2351 0.74 +1.2 2,373,594 + EX28DRAFT_2351 0.76 +0.6 2,373,607 - EX28DRAFT_2351 0.77 +1.3 2,373,735 + +0.5 2,373,740 - +0.9 2,373,749 - -0.1 2,373,890 - +0.3 2,373,899 + -0.1 2,373,900 - +0.8 2,373,930 + +0.6 2,373,949 + EX28DRAFT_2352 0.12 -0.0 2,373,950 - EX28DRAFT_2352 0.12 -0.4 2,373,953 + EX28DRAFT_2352 0.14 +0.3 2,374,081 + -2.3 2,374,159 - -1.2 2,374,287 - EX28DRAFT_2353 0.17 +0.1 2,374,287 - EX28DRAFT_2353 0.17 -0.8 2,374,361 + EX28DRAFT_2353 0.24 -0.1 2,374,362 - EX28DRAFT_2353 0.24 -0.4 2,374,532 + EX28DRAFT_2353 0.39 -0.3 2,374,532 + EX28DRAFT_2353 0.39 +0.4 2,374,653 - EX28DRAFT_2353 0.49 +0.3 2,374,862 + EX28DRAFT_2353 0.67 +0.7 2,374,863 - EX28DRAFT_2353 0.67 +0.1 2,374,871 + EX28DRAFT_2353 0.68 -0.9 2,374,932 - EX28DRAFT_2353 0.73 -1.2 2,374,962 + EX28DRAFT_2353 0.76 -0.4 2,374,963 - EX28DRAFT_2353 0.76 -0.1 2,375,085 - EX28DRAFT_2353 0.86 +0.9 2,375,101 + EX28DRAFT_2353 0.88 -0.5 2,375,101 + EX28DRAFT_2353 0.88 -0.7 2,375,102 - EX28DRAFT_2353 0.88 -3.0 2,375,102 - EX28DRAFT_2353 0.88 +0.1 2,375,102 - EX28DRAFT_2353 0.88 +0.6 2,375,193 + +1.6 2,375,262 + -2.6 2,375,269 + +1.1 2,375,269 + +1.0 2,375,270 - +0.1 2,375,319 + -0.4 2,375,334 + -0.4 2,375,334 + +0.2 2,375,453 + EX28DRAFT_2354 0.14 +0.3 2,375,610 - EX28DRAFT_2354 0.40 -0.3 2,375,610 - EX28DRAFT_2354 0.40 -0.3 2,375,846 + EX28DRAFT_2354 0.78 +0.3 2,375,846 + EX28DRAFT_2354 0.78 +0.7 2,375,847 - EX28DRAFT_2354 0.78 -2.0 2,375,878 + EX28DRAFT_2354 0.83 -0.6 2,375,929 + -1.0 2,375,930 - -0.4 2,375,939 - -0.2 2,376,193 + EX28DRAFT_2355 0.31 -0.0
Or see this region's nucleotide sequence