Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2296 and EX28DRAFT_2297 are separated by 370 nucleotides EX28DRAFT_2297 and EX28DRAFT_2298 are separated by 56 nucleotides
EX28DRAFT_2296: EX28DRAFT_2296 - PTS system, N-acetylglucosamine-specific IIBC component, at 2,324,420 to 2,326,399
_2296
EX28DRAFT_2297: EX28DRAFT_2297 - glucosamine-6-phosphate isomerase, at 2,326,770 to 2,327,570
_2297
EX28DRAFT_2298: EX28DRAFT_2298 - N-acetylglucosamine-6-phosphate deacetylase, at 2,327,627 to 2,328,775
_2298
Position (kb)
2326
2327
2328 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2325.924 kb on + strand, within EX28DRAFT_2296 at 2325.924 kb on + strand, within EX28DRAFT_2296 at 2325.924 kb on + strand, within EX28DRAFT_2296 at 2325.924 kb on + strand, within EX28DRAFT_2296 at 2325.924 kb on + strand, within EX28DRAFT_2296 at 2325.925 kb on - strand, within EX28DRAFT_2296 at 2325.925 kb on - strand, within EX28DRAFT_2296 at 2325.925 kb on - strand, within EX28DRAFT_2296 at 2326.038 kb on + strand, within EX28DRAFT_2296 at 2326.038 kb on + strand, within EX28DRAFT_2296 at 2326.139 kb on - strand, within EX28DRAFT_2296 at 2326.139 kb on - strand, within EX28DRAFT_2296 at 2326.294 kb on + strand at 2326.318 kb on + strand at 2326.318 kb on + strand at 2326.322 kb on - strand at 2326.493 kb on + strand at 2326.511 kb on - strand at 2326.511 kb on - strand at 2326.514 kb on + strand at 2326.628 kb on + strand at 2326.629 kb on - strand at 2326.629 kb on - strand at 2326.629 kb on - strand at 2326.705 kb on - strand at 2326.710 kb on + strand at 2326.710 kb on + strand at 2326.714 kb on + strand at 2326.715 kb on - strand at 2326.747 kb on + strand at 2326.748 kb on - strand at 2326.838 kb on + strand at 2326.937 kb on + strand, within EX28DRAFT_2297 at 2326.989 kb on + strand, within EX28DRAFT_2297 at 2326.989 kb on + strand, within EX28DRAFT_2297 at 2326.990 kb on - strand, within EX28DRAFT_2297 at 2327.022 kb on + strand, within EX28DRAFT_2297 at 2327.023 kb on - strand, within EX28DRAFT_2297 at 2327.023 kb on - strand, within EX28DRAFT_2297 at 2327.034 kb on - strand, within EX28DRAFT_2297 at 2327.131 kb on - strand, within EX28DRAFT_2297 at 2327.182 kb on + strand, within EX28DRAFT_2297 at 2327.183 kb on - strand, within EX28DRAFT_2297 at 2327.319 kb on + strand, within EX28DRAFT_2297 at 2327.435 kb on + strand, within EX28DRAFT_2297 at 2327.529 kb on + strand at 2327.568 kb on + strand at 2327.627 kb on - strand at 2327.627 kb on - strand at 2327.630 kb on + strand at 2327.630 kb on + strand at 2327.654 kb on + strand at 2327.776 kb on - strand, within EX28DRAFT_2298 at 2327.800 kb on - strand, within EX28DRAFT_2298 at 2327.800 kb on - strand, within EX28DRAFT_2298 at 2327.909 kb on + strand, within EX28DRAFT_2298 at 2327.909 kb on + strand, within EX28DRAFT_2298 at 2327.909 kb on + strand, within EX28DRAFT_2298 at 2327.910 kb on - strand, within EX28DRAFT_2298 at 2327.992 kb on + strand, within EX28DRAFT_2298 at 2328.065 kb on + strand, within EX28DRAFT_2298 at 2328.066 kb on - strand, within EX28DRAFT_2298 at 2328.073 kb on + strand, within EX28DRAFT_2298 at 2328.098 kb on + strand, within EX28DRAFT_2298 at 2328.098 kb on + strand, within EX28DRAFT_2298 at 2328.099 kb on - strand, within EX28DRAFT_2298 at 2328.293 kb on + strand, within EX28DRAFT_2298 at 2328.293 kb on + strand, within EX28DRAFT_2298 at 2328.350 kb on + strand, within EX28DRAFT_2298 at 2328.362 kb on - strand, within EX28DRAFT_2298 at 2328.375 kb on + strand, within EX28DRAFT_2298 at 2328.375 kb on + strand, within EX28DRAFT_2298 at 2328.376 kb on - strand, within EX28DRAFT_2298 at 2328.376 kb on - strand, within EX28DRAFT_2298 at 2328.376 kb on - strand, within EX28DRAFT_2298 at 2328.504 kb on - strand, within EX28DRAFT_2298
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,325,924 + EX28DRAFT_2296 0.76 -0.2 2,325,924 + EX28DRAFT_2296 0.76 -1.1 2,325,924 + EX28DRAFT_2296 0.76 -2.3 2,325,924 + EX28DRAFT_2296 0.76 +0.2 2,325,924 + EX28DRAFT_2296 0.76 -1.0 2,325,925 - EX28DRAFT_2296 0.76 -0.5 2,325,925 - EX28DRAFT_2296 0.76 +0.2 2,325,925 - EX28DRAFT_2296 0.76 +0.0 2,326,038 + EX28DRAFT_2296 0.82 -2.6 2,326,038 + EX28DRAFT_2296 0.82 +0.6 2,326,139 - EX28DRAFT_2296 0.87 -0.7 2,326,139 - EX28DRAFT_2296 0.87 -0.2 2,326,294 + +0.2 2,326,318 + +0.6 2,326,318 + -0.0 2,326,322 - +0.2 2,326,493 + +0.5 2,326,511 - -3.9 2,326,511 - -1.8 2,326,514 + +1.0 2,326,628 + -1.4 2,326,629 - +0.1 2,326,629 - -2.3 2,326,629 - +0.5 2,326,705 - +0.4 2,326,710 + -0.0 2,326,710 + -0.3 2,326,714 + +0.8 2,326,715 - -0.2 2,326,747 + -0.0 2,326,748 - +0.2 2,326,838 + -0.3 2,326,937 + EX28DRAFT_2297 0.21 +0.8 2,326,989 + EX28DRAFT_2297 0.27 -0.7 2,326,989 + EX28DRAFT_2297 0.27 +0.2 2,326,990 - EX28DRAFT_2297 0.27 +1.8 2,327,022 + EX28DRAFT_2297 0.31 -1.0 2,327,023 - EX28DRAFT_2297 0.32 +0.3 2,327,023 - EX28DRAFT_2297 0.32 -0.3 2,327,034 - EX28DRAFT_2297 0.33 +0.3 2,327,131 - EX28DRAFT_2297 0.45 -0.6 2,327,182 + EX28DRAFT_2297 0.51 +0.1 2,327,183 - EX28DRAFT_2297 0.52 -0.3 2,327,319 + EX28DRAFT_2297 0.69 -0.9 2,327,435 + EX28DRAFT_2297 0.83 -0.0 2,327,529 + +0.2 2,327,568 + +0.4 2,327,627 - +1.3 2,327,627 - +0.4 2,327,630 + +0.5 2,327,630 + -0.8 2,327,654 + +1.0 2,327,776 - EX28DRAFT_2298 0.13 +0.1 2,327,800 - EX28DRAFT_2298 0.15 -0.3 2,327,800 - EX28DRAFT_2298 0.15 +0.4 2,327,909 + EX28DRAFT_2298 0.25 -1.6 2,327,909 + EX28DRAFT_2298 0.25 -0.6 2,327,909 + EX28DRAFT_2298 0.25 +0.4 2,327,910 - EX28DRAFT_2298 0.25 -0.1 2,327,992 + EX28DRAFT_2298 0.32 -0.3 2,328,065 + EX28DRAFT_2298 0.38 -0.5 2,328,066 - EX28DRAFT_2298 0.38 +1.3 2,328,073 + EX28DRAFT_2298 0.39 -0.3 2,328,098 + EX28DRAFT_2298 0.41 -0.5 2,328,098 + EX28DRAFT_2298 0.41 -1.3 2,328,099 - EX28DRAFT_2298 0.41 -0.1 2,328,293 + EX28DRAFT_2298 0.58 -0.1 2,328,293 + EX28DRAFT_2298 0.58 -0.6 2,328,350 + EX28DRAFT_2298 0.63 +0.3 2,328,362 - EX28DRAFT_2298 0.64 -0.1 2,328,375 + EX28DRAFT_2298 0.65 +1.8 2,328,375 + EX28DRAFT_2298 0.65 +0.7 2,328,376 - EX28DRAFT_2298 0.65 +1.8 2,328,376 - EX28DRAFT_2298 0.65 +1.6 2,328,376 - EX28DRAFT_2298 0.65 -1.6 2,328,504 - EX28DRAFT_2298 0.76 +0.3
Or see this region's nucleotide sequence