Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2194 and EX28DRAFT_2195 are separated by 207 nucleotides EX28DRAFT_2195 and EX28DRAFT_2196 overlap by 4 nucleotides EX28DRAFT_2196 and EX28DRAFT_2197 overlap by 8 nucleotides
EX28DRAFT_2194: EX28DRAFT_2194 - Superfamily II DNA and RNA helicases, at 2,217,509 to 2,218,894
_2194
EX28DRAFT_2195: EX28DRAFT_2195 - transcriptional regulator, TetR family, at 2,219,102 to 2,219,779
_2195
EX28DRAFT_2196: EX28DRAFT_2196 - Multidrug resistance efflux pump, at 2,219,776 to 2,220,771
_2196
EX28DRAFT_2197: EX28DRAFT_2197 - ABC-type multidrug transport system, ATPase component, at 2,220,764 to 2,222,503
_2197
Position (kb)
2219
2220
2221 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2218.834 kb on - strand at 2218.895 kb on - strand at 2218.928 kb on + strand at 2218.943 kb on + strand at 2218.943 kb on + strand at 2218.943 kb on + strand at 2218.943 kb on + strand at 2218.944 kb on - strand at 2218.944 kb on - strand at 2218.944 kb on - strand at 2218.944 kb on - strand at 2218.945 kb on + strand at 2218.945 kb on + strand at 2218.945 kb on + strand at 2219.023 kb on + strand at 2219.064 kb on - strand at 2219.107 kb on + strand at 2219.253 kb on - strand, within EX28DRAFT_2195 at 2219.274 kb on + strand, within EX28DRAFT_2195 at 2219.274 kb on + strand, within EX28DRAFT_2195 at 2219.275 kb on - strand, within EX28DRAFT_2195 at 2219.275 kb on - strand, within EX28DRAFT_2195 at 2219.275 kb on - strand, within EX28DRAFT_2195 at 2219.417 kb on - strand, within EX28DRAFT_2195 at 2219.443 kb on + strand, within EX28DRAFT_2195 at 2219.444 kb on - strand, within EX28DRAFT_2195 at 2219.465 kb on - strand, within EX28DRAFT_2195 at 2219.564 kb on + strand, within EX28DRAFT_2195 at 2219.565 kb on - strand, within EX28DRAFT_2195 at 2219.566 kb on + strand, within EX28DRAFT_2195 at 2219.566 kb on + strand, within EX28DRAFT_2195 at 2219.866 kb on - strand at 2219.895 kb on + strand, within EX28DRAFT_2196 at 2219.911 kb on - strand, within EX28DRAFT_2196 at 2220.064 kb on + strand, within EX28DRAFT_2196 at 2220.065 kb on - strand, within EX28DRAFT_2196 at 2220.124 kb on - strand, within EX28DRAFT_2196 at 2220.278 kb on + strand, within EX28DRAFT_2196 at 2220.279 kb on - strand, within EX28DRAFT_2196 at 2220.393 kb on - strand, within EX28DRAFT_2196 at 2220.562 kb on + strand, within EX28DRAFT_2196 at 2220.564 kb on + strand, within EX28DRAFT_2196 at 2220.564 kb on + strand, within EX28DRAFT_2196 at 2220.740 kb on + strand at 2220.740 kb on + strand at 2220.863 kb on + strand at 2221.013 kb on + strand, within EX28DRAFT_2197 at 2221.013 kb on + strand, within EX28DRAFT_2197 at 2221.037 kb on + strand, within EX28DRAFT_2197 at 2221.139 kb on - strand, within EX28DRAFT_2197 at 2221.269 kb on + strand, within EX28DRAFT_2197 at 2221.270 kb on - strand, within EX28DRAFT_2197 at 2221.629 kb on + strand, within EX28DRAFT_2197 at 2221.720 kb on + strand, within EX28DRAFT_2197 at 2221.720 kb on + strand, within EX28DRAFT_2197 at 2221.721 kb on - strand, within EX28DRAFT_2197
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,218,834 - -0.7 2,218,895 - -0.1 2,218,928 + -0.4 2,218,943 + -0.4 2,218,943 + -0.0 2,218,943 + +0.0 2,218,943 + +0.5 2,218,944 - +0.3 2,218,944 - -0.4 2,218,944 - +0.0 2,218,944 - -0.2 2,218,945 + -1.7 2,218,945 + -0.0 2,218,945 + +0.3 2,219,023 + +0.3 2,219,064 - +0.3 2,219,107 + +0.6 2,219,253 - EX28DRAFT_2195 0.22 -0.7 2,219,274 + EX28DRAFT_2195 0.25 -1.1 2,219,274 + EX28DRAFT_2195 0.25 +0.0 2,219,275 - EX28DRAFT_2195 0.26 -0.0 2,219,275 - EX28DRAFT_2195 0.26 -1.1 2,219,275 - EX28DRAFT_2195 0.26 -1.6 2,219,417 - EX28DRAFT_2195 0.46 +1.9 2,219,443 + EX28DRAFT_2195 0.50 +0.9 2,219,444 - EX28DRAFT_2195 0.50 -0.5 2,219,465 - EX28DRAFT_2195 0.54 +0.1 2,219,564 + EX28DRAFT_2195 0.68 -2.4 2,219,565 - EX28DRAFT_2195 0.68 +1.5 2,219,566 + EX28DRAFT_2195 0.68 +0.5 2,219,566 + EX28DRAFT_2195 0.68 -1.1 2,219,866 - -1.7 2,219,895 + EX28DRAFT_2196 0.12 -1.8 2,219,911 - EX28DRAFT_2196 0.14 +1.1 2,220,064 + EX28DRAFT_2196 0.29 +0.0 2,220,065 - EX28DRAFT_2196 0.29 +1.0 2,220,124 - EX28DRAFT_2196 0.35 -0.9 2,220,278 + EX28DRAFT_2196 0.50 +0.7 2,220,279 - EX28DRAFT_2196 0.51 +0.3 2,220,393 - EX28DRAFT_2196 0.62 +0.7 2,220,562 + EX28DRAFT_2196 0.79 +0.7 2,220,564 + EX28DRAFT_2196 0.79 -0.6 2,220,564 + EX28DRAFT_2196 0.79 +0.4 2,220,740 + -0.1 2,220,740 + -0.7 2,220,863 + +0.7 2,221,013 + EX28DRAFT_2197 0.14 +0.3 2,221,013 + EX28DRAFT_2197 0.14 -0.1 2,221,037 + EX28DRAFT_2197 0.16 +0.5 2,221,139 - EX28DRAFT_2197 0.22 +0.5 2,221,269 + EX28DRAFT_2197 0.29 -0.0 2,221,270 - EX28DRAFT_2197 0.29 -0.1 2,221,629 + EX28DRAFT_2197 0.50 -0.2 2,221,720 + EX28DRAFT_2197 0.55 -1.2 2,221,720 + EX28DRAFT_2197 0.55 +2.3 2,221,721 - EX28DRAFT_2197 0.55 -0.0
Or see this region's nucleotide sequence