Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2092 and EX28DRAFT_2093 overlap by 17 nucleotides EX28DRAFT_2093 and EX28DRAFT_2094 are separated by 116 nucleotides EX28DRAFT_2094 and EX28DRAFT_2095 are separated by 40 nucleotides EX28DRAFT_2095 and EX28DRAFT_2096 are separated by 65 nucleotides
EX28DRAFT_2092: EX28DRAFT_2092 - His Kinase A (phospho-acceptor) domain/Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase, at 2,115,872 to 2,117,341
_2092
EX28DRAFT_2093: EX28DRAFT_2093 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 2,117,325 to 2,118,032
_2093
EX28DRAFT_2094: EX28DRAFT_2094 - 23S rRNA (uracil-5-)-methyltransferase RumB, at 2,118,149 to 2,119,279
_2094
EX28DRAFT_2095: EX28DRAFT_2095 - Protein of unknown function (DUF2593), at 2,119,320 to 2,119,793
_2095
EX28DRAFT_2096: EX28DRAFT_2096 - ABC-type spermidine/putrescine transport system, permease component II, at 2,119,859 to 2,120,704
_2096
Position (kb)
2118
2119
2120 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2117.183 kb on + strand, within EX28DRAFT_2092 at 2117.184 kb on - strand, within EX28DRAFT_2092 at 2117.362 kb on + strand at 2117.438 kb on + strand, within EX28DRAFT_2093 at 2117.438 kb on + strand, within EX28DRAFT_2093 at 2117.439 kb on - strand, within EX28DRAFT_2093 at 2117.447 kb on + strand, within EX28DRAFT_2093 at 2117.737 kb on + strand, within EX28DRAFT_2093 at 2118.056 kb on - strand at 2118.056 kb on - strand at 2118.056 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.063 kb on - strand at 2118.068 kb on + strand at 2118.193 kb on + strand at 2118.205 kb on + strand at 2118.288 kb on + strand, within EX28DRAFT_2094 at 2118.297 kb on + strand, within EX28DRAFT_2094 at 2118.321 kb on + strand, within EX28DRAFT_2094 at 2118.571 kb on + strand, within EX28DRAFT_2094 at 2118.571 kb on + strand, within EX28DRAFT_2094 at 2118.571 kb on + strand, within EX28DRAFT_2094 at 2118.572 kb on - strand, within EX28DRAFT_2094 at 2119.108 kb on + strand, within EX28DRAFT_2094 at 2119.229 kb on + strand at 2119.263 kb on + strand at 2119.280 kb on - strand at 2119.314 kb on + strand at 2119.314 kb on + strand at 2119.314 kb on + strand at 2119.315 kb on - strand at 2119.321 kb on + strand at 2119.489 kb on + strand, within EX28DRAFT_2095 at 2119.709 kb on + strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.710 kb on - strand, within EX28DRAFT_2095 at 2119.712 kb on + strand, within EX28DRAFT_2095 at 2119.746 kb on + strand at 2119.746 kb on + strand at 2119.747 kb on - strand at 2119.747 kb on - strand at 2119.752 kb on - strand at 2119.766 kb on + strand at 2119.766 kb on + strand at 2119.767 kb on - strand at 2119.767 kb on - strand at 2119.767 kb on - strand at 2119.770 kb on - strand at 2119.799 kb on + strand at 2119.963 kb on + strand, within EX28DRAFT_2096 at 2119.963 kb on + strand, within EX28DRAFT_2096 at 2119.964 kb on - strand, within EX28DRAFT_2096 at 2120.244 kb on + strand, within EX28DRAFT_2096 at 2120.244 kb on + strand, within EX28DRAFT_2096 at 2120.245 kb on - strand, within EX28DRAFT_2096 at 2120.252 kb on - strand, within EX28DRAFT_2096
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 2,117,183 + EX28DRAFT_2092 0.89 -0.4 2,117,184 - EX28DRAFT_2092 0.89 -0.5 2,117,362 + +0.4 2,117,438 + EX28DRAFT_2093 0.16 -0.3 2,117,438 + EX28DRAFT_2093 0.16 -0.1 2,117,439 - EX28DRAFT_2093 0.16 +1.4 2,117,447 + EX28DRAFT_2093 0.17 +0.3 2,117,737 + EX28DRAFT_2093 0.58 +0.7 2,118,056 - +0.3 2,118,056 - -0.1 2,118,056 - -0.4 2,118,063 - -0.2 2,118,063 - -0.7 2,118,063 - -0.9 2,118,063 - -0.8 2,118,068 + +0.8 2,118,193 + -0.3 2,118,205 + -0.3 2,118,288 + EX28DRAFT_2094 0.12 -0.3 2,118,297 + EX28DRAFT_2094 0.13 +0.6 2,118,321 + EX28DRAFT_2094 0.15 +0.2 2,118,571 + EX28DRAFT_2094 0.37 +1.5 2,118,571 + EX28DRAFT_2094 0.37 -0.5 2,118,571 + EX28DRAFT_2094 0.37 +0.3 2,118,572 - EX28DRAFT_2094 0.37 +0.1 2,119,108 + EX28DRAFT_2094 0.85 +0.3 2,119,229 + -0.8 2,119,263 + -0.6 2,119,280 - +0.4 2,119,314 + -0.3 2,119,314 + -0.3 2,119,314 + -0.3 2,119,315 - +1.6 2,119,321 + +2.3 2,119,489 + EX28DRAFT_2095 0.36 +0.3 2,119,709 + EX28DRAFT_2095 0.82 -0.0 2,119,710 - EX28DRAFT_2095 0.82 -2.3 2,119,710 - EX28DRAFT_2095 0.82 -0.5 2,119,710 - EX28DRAFT_2095 0.82 +0.9 2,119,712 + EX28DRAFT_2095 0.83 +0.4 2,119,746 + +0.1 2,119,746 + -0.7 2,119,747 - +0.0 2,119,747 - -0.6 2,119,752 - +0.1 2,119,766 + +0.2 2,119,766 + +0.3 2,119,767 - +0.2 2,119,767 - +0.5 2,119,767 - -2.7 2,119,770 - -0.9 2,119,799 + +0.2 2,119,963 + EX28DRAFT_2096 0.12 -1.1 2,119,963 + EX28DRAFT_2096 0.12 +0.8 2,119,964 - EX28DRAFT_2096 0.12 +0.5 2,120,244 + EX28DRAFT_2096 0.46 +0.5 2,120,244 + EX28DRAFT_2096 0.46 -1.2 2,120,245 - EX28DRAFT_2096 0.46 +0.2 2,120,252 - EX28DRAFT_2096 0.46 +0.3
Or see this region's nucleotide sequence