Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1947 and EX28DRAFT_1948 are separated by 102 nucleotides EX28DRAFT_1948 and EX28DRAFT_1949 overlap by 21 nucleotides EX28DRAFT_1949 and EX28DRAFT_1950 overlap by 4 nucleotides
EX28DRAFT_1947: EX28DRAFT_1947 - Cytosine/uracil/thiamine/allantoin permeases, at 1,951,947 to 1,953,443
_1947
EX28DRAFT_1948: EX28DRAFT_1948 - guanine deaminase (EC 3.5.4.3), at 1,953,546 to 1,954,862
_1948
EX28DRAFT_1949: EX28DRAFT_1949 - hydroxyisourate hydrolase, at 1,954,842 to 1,955,165
_1949
EX28DRAFT_1950: EX28DRAFT_1950 - OHCU decarboxylase, at 1,955,162 to 1,955,662
_1950
Position (kb)
1953
1954
1955 Strain fitness (log2 ratio)
-2
-1
0
1 at 1952.577 kb on + strand, within EX28DRAFT_1947 at 1952.578 kb on - strand, within EX28DRAFT_1947 at 1952.682 kb on + strand, within EX28DRAFT_1947 at 1952.683 kb on - strand, within EX28DRAFT_1947 at 1952.982 kb on - strand, within EX28DRAFT_1947 at 1953.154 kb on - strand, within EX28DRAFT_1947 at 1953.161 kb on + strand, within EX28DRAFT_1947 at 1953.161 kb on + strand, within EX28DRAFT_1947 at 1953.216 kb on + strand, within EX28DRAFT_1947 at 1953.216 kb on + strand, within EX28DRAFT_1947 at 1953.216 kb on + strand, within EX28DRAFT_1947 at 1953.216 kb on + strand, within EX28DRAFT_1947 at 1953.216 kb on + strand, within EX28DRAFT_1947 at 1953.216 kb on + strand, within EX28DRAFT_1947 at 1953.290 kb on + strand, within EX28DRAFT_1947 at 1953.291 kb on - strand, within EX28DRAFT_1947 at 1953.363 kb on + strand at 1953.585 kb on - strand at 1953.657 kb on - strand at 1953.658 kb on + strand at 1953.659 kb on - strand at 1953.708 kb on + strand, within EX28DRAFT_1948 at 1953.846 kb on + strand, within EX28DRAFT_1948 at 1953.846 kb on + strand, within EX28DRAFT_1948 at 1953.847 kb on - strand, within EX28DRAFT_1948 at 1954.563 kb on + strand, within EX28DRAFT_1948 at 1954.563 kb on + strand, within EX28DRAFT_1948 at 1954.564 kb on - strand, within EX28DRAFT_1948 at 1954.761 kb on + strand at 1954.761 kb on + strand at 1954.762 kb on - strand at 1955.094 kb on + strand, within EX28DRAFT_1949 at 1955.094 kb on + strand, within EX28DRAFT_1949 at 1955.095 kb on - strand, within EX28DRAFT_1949 at 1955.095 kb on - strand, within EX28DRAFT_1949 at 1955.095 kb on - strand, within EX28DRAFT_1949 at 1955.095 kb on - strand, within EX28DRAFT_1949 at 1955.145 kb on - strand at 1955.198 kb on - strand at 1955.274 kb on - strand, within EX28DRAFT_1950 at 1955.279 kb on - strand, within EX28DRAFT_1950 at 1955.662 kb on + strand at 1955.662 kb on + strand at 1955.662 kb on + strand at 1955.662 kb on + strand at 1955.662 kb on + strand at 1955.663 kb on - strand at 1955.663 kb on - strand at 1955.675 kb on - strand at 1955.746 kb on - strand at 1955.803 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,952,577 + EX28DRAFT_1947 0.42 -0.5 1,952,578 - EX28DRAFT_1947 0.42 +0.0 1,952,682 + EX28DRAFT_1947 0.49 -0.3 1,952,683 - EX28DRAFT_1947 0.49 +0.4 1,952,982 - EX28DRAFT_1947 0.69 +0.1 1,953,154 - EX28DRAFT_1947 0.81 +0.9 1,953,161 + EX28DRAFT_1947 0.81 -0.5 1,953,161 + EX28DRAFT_1947 0.81 -0.1 1,953,216 + EX28DRAFT_1947 0.85 -0.1 1,953,216 + EX28DRAFT_1947 0.85 -0.4 1,953,216 + EX28DRAFT_1947 0.85 +0.5 1,953,216 + EX28DRAFT_1947 0.85 +0.0 1,953,216 + EX28DRAFT_1947 0.85 -0.1 1,953,216 + EX28DRAFT_1947 0.85 +1.0 1,953,290 + EX28DRAFT_1947 0.90 +0.1 1,953,291 - EX28DRAFT_1947 0.90 +0.3 1,953,363 + +0.8 1,953,585 - -0.1 1,953,657 - -0.1 1,953,658 + -0.4 1,953,659 - -0.6 1,953,708 + EX28DRAFT_1948 0.12 +0.3 1,953,846 + EX28DRAFT_1948 0.23 +0.8 1,953,846 + EX28DRAFT_1948 0.23 +0.8 1,953,847 - EX28DRAFT_1948 0.23 -0.3 1,954,563 + EX28DRAFT_1948 0.77 +0.2 1,954,563 + EX28DRAFT_1948 0.77 +1.0 1,954,564 - EX28DRAFT_1948 0.77 -2.5 1,954,761 + +0.4 1,954,761 + -0.1 1,954,762 - -0.8 1,955,094 + EX28DRAFT_1949 0.78 +0.5 1,955,094 + EX28DRAFT_1949 0.78 +0.3 1,955,095 - EX28DRAFT_1949 0.78 -0.7 1,955,095 - EX28DRAFT_1949 0.78 +0.4 1,955,095 - EX28DRAFT_1949 0.78 -0.8 1,955,095 - EX28DRAFT_1949 0.78 +0.4 1,955,145 - -1.8 1,955,198 - +0.2 1,955,274 - EX28DRAFT_1950 0.22 +0.2 1,955,279 - EX28DRAFT_1950 0.23 -0.8 1,955,662 + -0.4 1,955,662 + -0.1 1,955,662 + +0.3 1,955,662 + +0.0 1,955,662 + -0.5 1,955,663 - +1.4 1,955,663 - -0.0 1,955,675 - -0.3 1,955,746 - +1.3 1,955,803 + +1.4
Or see this region's nucleotide sequence