Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1934 and EX28DRAFT_1935 are separated by 13 nucleotides EX28DRAFT_1935 and EX28DRAFT_1936 overlap by 20 nucleotides EX28DRAFT_1936 and EX28DRAFT_1937 overlap by 22 nucleotides EX28DRAFT_1937 and EX28DRAFT_1938 are separated by 4 nucleotides
EX28DRAFT_1934: EX28DRAFT_1934 - Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase, at 1,940,511 to 1,941,752
_1934
EX28DRAFT_1935: EX28DRAFT_1935 - ABC-type polar amino acid transport system, ATPase component, at 1,941,766 to 1,942,503
_1935
EX28DRAFT_1936: EX28DRAFT_1936 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 1,942,484 to 1,943,161
_1936
EX28DRAFT_1937: EX28DRAFT_1937 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 1,943,140 to 1,943,805
_1937
EX28DRAFT_1938: EX28DRAFT_1938 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,943,810 to 1,944,592
_1938
Position (kb)
1942
1943
1944 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1941.485 kb on + strand, within EX28DRAFT_1934 at 1941.485 kb on + strand, within EX28DRAFT_1934 at 1941.614 kb on - strand, within EX28DRAFT_1934 at 1941.614 kb on - strand, within EX28DRAFT_1934 at 1941.614 kb on - strand, within EX28DRAFT_1934 at 1941.757 kb on + strand at 1941.874 kb on - strand, within EX28DRAFT_1935 at 1941.928 kb on + strand, within EX28DRAFT_1935 at 1941.928 kb on + strand, within EX28DRAFT_1935 at 1942.104 kb on - strand, within EX28DRAFT_1935 at 1942.104 kb on - strand, within EX28DRAFT_1935 at 1942.469 kb on + strand at 1942.469 kb on + strand at 1942.470 kb on - strand at 1942.486 kb on - strand at 1942.555 kb on + strand, within EX28DRAFT_1936 at 1942.556 kb on - strand, within EX28DRAFT_1936 at 1942.556 kb on - strand, within EX28DRAFT_1936 at 1942.600 kb on - strand, within EX28DRAFT_1936 at 1942.676 kb on - strand, within EX28DRAFT_1936 at 1942.676 kb on - strand, within EX28DRAFT_1936 at 1942.854 kb on + strand, within EX28DRAFT_1936 at 1942.854 kb on + strand, within EX28DRAFT_1936 at 1942.854 kb on + strand, within EX28DRAFT_1936 at 1942.973 kb on - strand, within EX28DRAFT_1936 at 1942.973 kb on - strand, within EX28DRAFT_1936 at 1942.975 kb on - strand, within EX28DRAFT_1936 at 1942.975 kb on - strand, within EX28DRAFT_1936 at 1943.114 kb on - strand at 1943.209 kb on + strand, within EX28DRAFT_1937 at 1943.209 kb on + strand, within EX28DRAFT_1937 at 1943.209 kb on + strand, within EX28DRAFT_1937 at 1943.210 kb on - strand, within EX28DRAFT_1937 at 1943.214 kb on - strand, within EX28DRAFT_1937 at 1943.235 kb on - strand, within EX28DRAFT_1937 at 1943.692 kb on + strand, within EX28DRAFT_1937 at 1943.971 kb on + strand, within EX28DRAFT_1938 at 1943.972 kb on - strand, within EX28DRAFT_1938 at 1944.045 kb on + strand, within EX28DRAFT_1938 at 1944.045 kb on + strand, within EX28DRAFT_1938 at 1944.046 kb on - strand, within EX28DRAFT_1938
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,941,485 + EX28DRAFT_1934 0.78 +0.4 1,941,485 + EX28DRAFT_1934 0.78 -0.1 1,941,614 - EX28DRAFT_1934 0.89 +0.8 1,941,614 - EX28DRAFT_1934 0.89 +0.4 1,941,614 - EX28DRAFT_1934 0.89 -0.2 1,941,757 + -0.8 1,941,874 - EX28DRAFT_1935 0.15 +0.1 1,941,928 + EX28DRAFT_1935 0.22 +0.4 1,941,928 + EX28DRAFT_1935 0.22 +0.5 1,942,104 - EX28DRAFT_1935 0.46 -0.1 1,942,104 - EX28DRAFT_1935 0.46 +0.3 1,942,469 + -2.2 1,942,469 + -0.0 1,942,470 - +0.4 1,942,486 - +0.2 1,942,555 + EX28DRAFT_1936 0.10 +2.2 1,942,556 - EX28DRAFT_1936 0.11 +0.5 1,942,556 - EX28DRAFT_1936 0.11 +1.3 1,942,600 - EX28DRAFT_1936 0.17 +0.0 1,942,676 - EX28DRAFT_1936 0.28 -0.2 1,942,676 - EX28DRAFT_1936 0.28 +0.3 1,942,854 + EX28DRAFT_1936 0.55 -1.0 1,942,854 + EX28DRAFT_1936 0.55 -1.0 1,942,854 + EX28DRAFT_1936 0.55 +0.2 1,942,973 - EX28DRAFT_1936 0.72 -0.0 1,942,973 - EX28DRAFT_1936 0.72 +0.1 1,942,975 - EX28DRAFT_1936 0.72 +0.6 1,942,975 - EX28DRAFT_1936 0.72 -0.1 1,943,114 - -0.1 1,943,209 + EX28DRAFT_1937 0.10 -0.4 1,943,209 + EX28DRAFT_1937 0.10 -0.3 1,943,209 + EX28DRAFT_1937 0.10 +0.2 1,943,210 - EX28DRAFT_1937 0.11 -2.7 1,943,214 - EX28DRAFT_1937 0.11 -1.2 1,943,235 - EX28DRAFT_1937 0.14 +0.8 1,943,692 + EX28DRAFT_1937 0.83 +1.3 1,943,971 + EX28DRAFT_1938 0.21 +1.2 1,943,972 - EX28DRAFT_1938 0.21 +0.3 1,944,045 + EX28DRAFT_1938 0.30 -1.0 1,944,045 + EX28DRAFT_1938 0.30 +0.1 1,944,046 - EX28DRAFT_1938 0.30 -0.1
Or see this region's nucleotide sequence