Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1805 and EX28DRAFT_1806 overlap by 4 nucleotides EX28DRAFT_1806 and EX28DRAFT_1807 overlap by 4 nucleotides EX28DRAFT_1807 and EX28DRAFT_1808 are separated by 46 nucleotides
EX28DRAFT_1805: EX28DRAFT_1805 - ABC-type spermidine/putrescine transport system, permease component I, at 1,819,995 to 1,820,852
_1805
EX28DRAFT_1806: EX28DRAFT_1806 - ABC-type spermidine/putrescine transport system, permease component II, at 1,820,849 to 1,821,640
_1806
EX28DRAFT_1807: EX28DRAFT_1807 - Spermidine/putrescine-binding periplasmic protein, at 1,821,637 to 1,822,671
_1807
EX28DRAFT_1808: EX28DRAFT_1808 - Predicted signal transduction protein containing EAL and modified HD-GYP domains, at 1,822,718 to 1,823,947
_1808
Position (kb)
1821
1822
1823 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1820.637 kb on - strand, within EX28DRAFT_1805 at 1820.661 kb on + strand, within EX28DRAFT_1805 at 1820.831 kb on + strand at 1820.892 kb on - strand at 1820.897 kb on + strand at 1820.898 kb on - strand at 1820.898 kb on - strand at 1820.903 kb on + strand at 1820.903 kb on + strand at 1820.903 kb on + strand at 1820.903 kb on + strand at 1820.960 kb on - strand, within EX28DRAFT_1806 at 1820.975 kb on - strand, within EX28DRAFT_1806 at 1820.987 kb on + strand, within EX28DRAFT_1806 at 1821.205 kb on + strand, within EX28DRAFT_1806 at 1821.421 kb on + strand, within EX28DRAFT_1806 at 1821.503 kb on + strand, within EX28DRAFT_1806 at 1821.504 kb on - strand, within EX28DRAFT_1806 at 1821.733 kb on + strand at 1821.800 kb on - strand, within EX28DRAFT_1807 at 1821.820 kb on + strand, within EX28DRAFT_1807 at 1821.820 kb on + strand, within EX28DRAFT_1807 at 1821.821 kb on - strand, within EX28DRAFT_1807 at 1821.877 kb on + strand, within EX28DRAFT_1807 at 1821.877 kb on + strand, within EX28DRAFT_1807 at 1821.877 kb on + strand, within EX28DRAFT_1807 at 1821.935 kb on + strand, within EX28DRAFT_1807 at 1821.936 kb on - strand, within EX28DRAFT_1807 at 1822.009 kb on + strand, within EX28DRAFT_1807 at 1822.261 kb on - strand, within EX28DRAFT_1807 at 1822.276 kb on + strand, within EX28DRAFT_1807 at 1822.277 kb on - strand, within EX28DRAFT_1807 at 1822.279 kb on - strand, within EX28DRAFT_1807 at 1822.324 kb on + strand, within EX28DRAFT_1807 at 1822.325 kb on - strand, within EX28DRAFT_1807 at 1822.325 kb on - strand, within EX28DRAFT_1807 at 1822.449 kb on + strand, within EX28DRAFT_1807 at 1822.547 kb on + strand, within EX28DRAFT_1807 at 1822.557 kb on + strand, within EX28DRAFT_1807 at 1822.642 kb on + strand at 1822.642 kb on + strand at 1822.643 kb on - strand at 1822.720 kb on - strand at 1823.207 kb on - strand, within EX28DRAFT_1808 at 1823.239 kb on + strand, within EX28DRAFT_1808 at 1823.363 kb on - strand, within EX28DRAFT_1808
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,820,637 - EX28DRAFT_1805 0.75 +0.2 1,820,661 + EX28DRAFT_1805 0.78 +0.5 1,820,831 + +0.1 1,820,892 - +0.3 1,820,897 + +1.7 1,820,898 - +0.1 1,820,898 - -0.1 1,820,903 + -0.4 1,820,903 + +0.5 1,820,903 + +0.9 1,820,903 + +0.2 1,820,960 - EX28DRAFT_1806 0.14 -0.8 1,820,975 - EX28DRAFT_1806 0.16 +1.1 1,820,987 + EX28DRAFT_1806 0.17 -0.0 1,821,205 + EX28DRAFT_1806 0.45 +0.1 1,821,421 + EX28DRAFT_1806 0.72 -0.3 1,821,503 + EX28DRAFT_1806 0.83 -2.7 1,821,504 - EX28DRAFT_1806 0.83 -0.7 1,821,733 + +0.6 1,821,800 - EX28DRAFT_1807 0.16 -2.3 1,821,820 + EX28DRAFT_1807 0.18 -4.2 1,821,820 + EX28DRAFT_1807 0.18 +0.1 1,821,821 - EX28DRAFT_1807 0.18 +2.8 1,821,877 + EX28DRAFT_1807 0.23 +0.4 1,821,877 + EX28DRAFT_1807 0.23 -0.1 1,821,877 + EX28DRAFT_1807 0.23 -1.1 1,821,935 + EX28DRAFT_1807 0.29 +0.1 1,821,936 - EX28DRAFT_1807 0.29 -0.3 1,822,009 + EX28DRAFT_1807 0.36 -0.3 1,822,261 - EX28DRAFT_1807 0.60 +0.1 1,822,276 + EX28DRAFT_1807 0.62 -0.7 1,822,277 - EX28DRAFT_1807 0.62 -1.0 1,822,279 - EX28DRAFT_1807 0.62 +0.3 1,822,324 + EX28DRAFT_1807 0.66 +0.1 1,822,325 - EX28DRAFT_1807 0.66 -0.6 1,822,325 - EX28DRAFT_1807 0.66 -0.7 1,822,449 + EX28DRAFT_1807 0.78 -0.2 1,822,547 + EX28DRAFT_1807 0.88 +0.0 1,822,557 + EX28DRAFT_1807 0.89 -1.9 1,822,642 + -0.4 1,822,642 + +0.8 1,822,643 - +0.0 1,822,720 - +0.3 1,823,207 - EX28DRAFT_1808 0.40 +0.5 1,823,239 + EX28DRAFT_1808 0.42 -0.7 1,823,363 - EX28DRAFT_1808 0.52 +0.5
Or see this region's nucleotide sequence