Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1741 and EX28DRAFT_1742 are separated by 79 nucleotides EX28DRAFT_1742 and EX28DRAFT_1743 are separated by 280 nucleotides EX28DRAFT_1743 and EX28DRAFT_1744 are separated by 232 nucleotides
EX28DRAFT_1741: EX28DRAFT_1741 - phosphotransferase system, cellobiose specific, IIC component, at 1,756,108 to 1,757,466
_1741
EX28DRAFT_1742: EX28DRAFT_1742 - PTS system, lactose/cellobiose family IIB component, at 1,757,546 to 1,757,863
_1742
EX28DRAFT_1743: EX28DRAFT_1743 - SmpA / OmlA family, at 1,758,144 to 1,758,488
_1743
EX28DRAFT_1744: EX28DRAFT_1744 - NAD+ synthetase, at 1,758,721 to 1,759,548
_1744
Position (kb)
1757
1758 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1756.563 kb on + strand, within EX28DRAFT_1741 at 1756.565 kb on + strand, within EX28DRAFT_1741 at 1756.565 kb on + strand, within EX28DRAFT_1741 at 1756.565 kb on + strand, within EX28DRAFT_1741 at 1756.565 kb on + strand, within EX28DRAFT_1741 at 1756.566 kb on - strand, within EX28DRAFT_1741 at 1756.566 kb on - strand, within EX28DRAFT_1741 at 1756.681 kb on - strand, within EX28DRAFT_1741 at 1756.758 kb on - strand, within EX28DRAFT_1741 at 1756.803 kb on + strand, within EX28DRAFT_1741 at 1756.804 kb on - strand, within EX28DRAFT_1741 at 1756.882 kb on - strand, within EX28DRAFT_1741 at 1756.971 kb on + strand, within EX28DRAFT_1741 at 1756.971 kb on + strand, within EX28DRAFT_1741 at 1756.971 kb on + strand, within EX28DRAFT_1741 at 1756.972 kb on - strand, within EX28DRAFT_1741 at 1757.058 kb on - strand, within EX28DRAFT_1741 at 1757.110 kb on + strand, within EX28DRAFT_1741 at 1757.187 kb on + strand, within EX28DRAFT_1741 at 1757.199 kb on + strand, within EX28DRAFT_1741 at 1757.200 kb on - strand, within EX28DRAFT_1741 at 1757.276 kb on - strand, within EX28DRAFT_1741 at 1757.282 kb on + strand, within EX28DRAFT_1741 at 1757.323 kb on - strand, within EX28DRAFT_1741 at 1757.411 kb on - strand at 1757.461 kb on - strand at 1757.481 kb on - strand at 1757.585 kb on - strand, within EX28DRAFT_1742 at 1757.677 kb on + strand, within EX28DRAFT_1742 at 1757.677 kb on + strand, within EX28DRAFT_1742 at 1757.677 kb on + strand, within EX28DRAFT_1742 at 1757.677 kb on + strand, within EX28DRAFT_1742 at 1757.679 kb on + strand, within EX28DRAFT_1742 at 1757.845 kb on - strand at 1757.845 kb on - strand at 1757.848 kb on + strand at 1757.848 kb on + strand at 1757.848 kb on + strand at 1757.848 kb on + strand at 1757.848 kb on + strand at 1757.849 kb on - strand at 1757.849 kb on - strand at 1757.877 kb on + strand at 1757.877 kb on + strand at 1757.877 kb on + strand at 1757.878 kb on - strand at 1757.879 kb on + strand at 1757.880 kb on - strand at 1757.880 kb on - strand at 1757.880 kb on - strand at 1757.880 kb on - strand at 1757.880 kb on - strand at 1757.932 kb on - strand at 1757.932 kb on - strand at 1757.941 kb on - strand at 1757.965 kb on + strand at 1757.966 kb on - strand at 1757.967 kb on + strand at 1757.968 kb on - strand at 1757.968 kb on - strand at 1757.968 kb on - strand at 1757.968 kb on - strand at 1758.156 kb on - strand at 1758.241 kb on + strand, within EX28DRAFT_1743 at 1758.241 kb on + strand, within EX28DRAFT_1743 at 1758.242 kb on - strand, within EX28DRAFT_1743 at 1758.275 kb on + strand, within EX28DRAFT_1743 at 1758.275 kb on + strand, within EX28DRAFT_1743 at 1758.275 kb on + strand, within EX28DRAFT_1743 at 1758.277 kb on + strand, within EX28DRAFT_1743 at 1758.277 kb on + strand, within EX28DRAFT_1743 at 1758.277 kb on + strand, within EX28DRAFT_1743 at 1758.278 kb on - strand, within EX28DRAFT_1743 at 1758.278 kb on - strand, within EX28DRAFT_1743 at 1758.382 kb on - strand, within EX28DRAFT_1743 at 1758.385 kb on - strand, within EX28DRAFT_1743 at 1758.425 kb on + strand, within EX28DRAFT_1743 at 1758.440 kb on + strand, within EX28DRAFT_1743 at 1758.440 kb on + strand, within EX28DRAFT_1743 at 1758.475 kb on - strand at 1758.475 kb on - strand at 1758.475 kb on - strand at 1758.521 kb on - strand at 1758.542 kb on - strand at 1758.683 kb on + strand at 1758.697 kb on + strand at 1758.701 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,756,563 + EX28DRAFT_1741 0.33 -0.4 1,756,565 + EX28DRAFT_1741 0.34 +0.0 1,756,565 + EX28DRAFT_1741 0.34 +0.6 1,756,565 + EX28DRAFT_1741 0.34 -0.4 1,756,565 + EX28DRAFT_1741 0.34 +0.7 1,756,566 - EX28DRAFT_1741 0.34 +0.1 1,756,566 - EX28DRAFT_1741 0.34 -0.3 1,756,681 - EX28DRAFT_1741 0.42 -0.4 1,756,758 - EX28DRAFT_1741 0.48 -0.2 1,756,803 + EX28DRAFT_1741 0.51 +0.2 1,756,804 - EX28DRAFT_1741 0.51 -0.2 1,756,882 - EX28DRAFT_1741 0.57 +0.1 1,756,971 + EX28DRAFT_1741 0.64 +0.8 1,756,971 + EX28DRAFT_1741 0.64 -0.6 1,756,971 + EX28DRAFT_1741 0.64 +0.5 1,756,972 - EX28DRAFT_1741 0.64 +0.1 1,757,058 - EX28DRAFT_1741 0.70 +0.3 1,757,110 + EX28DRAFT_1741 0.74 +0.2 1,757,187 + EX28DRAFT_1741 0.79 -0.5 1,757,199 + EX28DRAFT_1741 0.80 +0.9 1,757,200 - EX28DRAFT_1741 0.80 +0.1 1,757,276 - EX28DRAFT_1741 0.86 +0.8 1,757,282 + EX28DRAFT_1741 0.86 +0.2 1,757,323 - EX28DRAFT_1741 0.89 +0.1 1,757,411 - +1.2 1,757,461 - -0.2 1,757,481 - -0.7 1,757,585 - EX28DRAFT_1742 0.12 -1.4 1,757,677 + EX28DRAFT_1742 0.41 -2.0 1,757,677 + EX28DRAFT_1742 0.41 +0.5 1,757,677 + EX28DRAFT_1742 0.41 -1.9 1,757,677 + EX28DRAFT_1742 0.41 -0.4 1,757,679 + EX28DRAFT_1742 0.42 -1.6 1,757,845 - -0.5 1,757,845 - -2.2 1,757,848 + -0.1 1,757,848 + +1.5 1,757,848 + +1.2 1,757,848 + -0.4 1,757,848 + +2.3 1,757,849 - +0.3 1,757,849 - -0.1 1,757,877 + -0.4 1,757,877 + -0.5 1,757,877 + -1.7 1,757,878 - -0.4 1,757,879 + +0.3 1,757,880 - -0.5 1,757,880 - -0.1 1,757,880 - +0.7 1,757,880 - +1.0 1,757,880 - -4.6 1,757,932 - -0.4 1,757,932 - -0.5 1,757,941 - -1.5 1,757,965 + -0.1 1,757,966 - -1.1 1,757,967 + +0.2 1,757,968 - +0.2 1,757,968 - +0.1 1,757,968 - -2.0 1,757,968 - -0.3 1,758,156 - +0.1 1,758,241 + EX28DRAFT_1743 0.28 -0.3 1,758,241 + EX28DRAFT_1743 0.28 +0.5 1,758,242 - EX28DRAFT_1743 0.28 -0.3 1,758,275 + EX28DRAFT_1743 0.38 -0.4 1,758,275 + EX28DRAFT_1743 0.38 -3.2 1,758,275 + EX28DRAFT_1743 0.38 -0.4 1,758,277 + EX28DRAFT_1743 0.39 +0.1 1,758,277 + EX28DRAFT_1743 0.39 +0.9 1,758,277 + EX28DRAFT_1743 0.39 -0.4 1,758,278 - EX28DRAFT_1743 0.39 +0.0 1,758,278 - EX28DRAFT_1743 0.39 +0.4 1,758,382 - EX28DRAFT_1743 0.69 -0.5 1,758,385 - EX28DRAFT_1743 0.70 +0.3 1,758,425 + EX28DRAFT_1743 0.81 +1.1 1,758,440 + EX28DRAFT_1743 0.86 +0.4 1,758,440 + EX28DRAFT_1743 0.86 -0.3 1,758,475 - +0.2 1,758,475 - -0.4 1,758,475 - +1.5 1,758,521 - +0.6 1,758,542 - -0.5 1,758,683 + -0.2 1,758,697 + +0.8 1,758,701 + +0.1
Or see this region's nucleotide sequence