Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1704 and EX28DRAFT_1705 are separated by 335 nucleotides EX28DRAFT_1705 and EX28DRAFT_1706 are separated by 152 nucleotides
EX28DRAFT_1704: EX28DRAFT_1704 - phosphoenolpyruvate synthase (EC 2.7.9.2), at 1,718,840 to 1,721,218
_1704
EX28DRAFT_1705: EX28DRAFT_1705 - Uncharacterized protein conserved in bacteria, at 1,721,554 to 1,722,387
_1705
EX28DRAFT_1706: EX28DRAFT_1706 - 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase (EC 2.5.1.54), at 1,722,540 to 1,723,586
_1706
Position (kb)
1721
1722
1723 Strain fitness (log2 ratio)
-2
-1
0
1 at 1720.559 kb on - strand, within EX28DRAFT_1704 at 1720.816 kb on + strand, within EX28DRAFT_1704 at 1720.987 kb on + strand at 1721.010 kb on + strand at 1721.157 kb on - strand at 1721.182 kb on - strand at 1721.267 kb on - strand at 1721.267 kb on - strand at 1721.270 kb on + strand at 1721.270 kb on + strand at 1721.270 kb on + strand at 1721.271 kb on - strand at 1721.275 kb on - strand at 1721.285 kb on - strand at 1721.288 kb on + strand at 1721.289 kb on - strand at 1721.528 kb on + strand at 1721.584 kb on + strand at 1721.732 kb on - strand, within EX28DRAFT_1705 at 1721.764 kb on + strand, within EX28DRAFT_1705 at 1721.764 kb on + strand, within EX28DRAFT_1705 at 1721.764 kb on + strand, within EX28DRAFT_1705 at 1721.764 kb on + strand, within EX28DRAFT_1705 at 1721.764 kb on + strand, within EX28DRAFT_1705 at 1721.764 kb on + strand, within EX28DRAFT_1705 at 1721.765 kb on - strand, within EX28DRAFT_1705 at 1721.765 kb on - strand, within EX28DRAFT_1705 at 1721.769 kb on + strand, within EX28DRAFT_1705 at 1721.770 kb on - strand, within EX28DRAFT_1705 at 1721.925 kb on + strand, within EX28DRAFT_1705 at 1721.925 kb on + strand, within EX28DRAFT_1705 at 1721.957 kb on - strand, within EX28DRAFT_1705 at 1722.058 kb on + strand, within EX28DRAFT_1705 at 1722.322 kb on + strand at 1722.323 kb on - strand at 1722.323 kb on - strand at 1722.396 kb on + strand at 1722.416 kb on + strand at 1722.545 kb on + strand at 1722.546 kb on - strand at 1722.546 kb on - strand at 1722.751 kb on - strand, within EX28DRAFT_1706 at 1722.817 kb on - strand, within EX28DRAFT_1706 at 1722.928 kb on + strand, within EX28DRAFT_1706 at 1722.929 kb on - strand, within EX28DRAFT_1706 at 1723.197 kb on + strand, within EX28DRAFT_1706 at 1723.198 kb on - strand, within EX28DRAFT_1706 at 1723.232 kb on + strand, within EX28DRAFT_1706 at 1723.233 kb on - strand, within EX28DRAFT_1706 at 1723.257 kb on + strand, within EX28DRAFT_1706 at 1723.257 kb on + strand, within EX28DRAFT_1706 at 1723.271 kb on + strand, within EX28DRAFT_1706
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,720,559 - EX28DRAFT_1704 0.72 +0.4 1,720,816 + EX28DRAFT_1704 0.83 -0.2 1,720,987 + +0.0 1,721,010 + +0.0 1,721,157 - +0.8 1,721,182 - +0.3 1,721,267 - -0.7 1,721,267 - +0.2 1,721,270 + -2.2 1,721,270 + -0.5 1,721,270 + -0.2 1,721,271 - +1.5 1,721,275 - +0.4 1,721,285 - +0.0 1,721,288 + -1.2 1,721,289 - +0.3 1,721,528 + +0.0 1,721,584 + +0.6 1,721,732 - EX28DRAFT_1705 0.21 -0.5 1,721,764 + EX28DRAFT_1705 0.25 -0.6 1,721,764 + EX28DRAFT_1705 0.25 -0.5 1,721,764 + EX28DRAFT_1705 0.25 +0.7 1,721,764 + EX28DRAFT_1705 0.25 -0.5 1,721,764 + EX28DRAFT_1705 0.25 +0.5 1,721,764 + EX28DRAFT_1705 0.25 +0.7 1,721,765 - EX28DRAFT_1705 0.25 +0.2 1,721,765 - EX28DRAFT_1705 0.25 +0.1 1,721,769 + EX28DRAFT_1705 0.26 -0.4 1,721,770 - EX28DRAFT_1705 0.26 -1.4 1,721,925 + EX28DRAFT_1705 0.44 -0.3 1,721,925 + EX28DRAFT_1705 0.44 +0.4 1,721,957 - EX28DRAFT_1705 0.48 -0.5 1,722,058 + EX28DRAFT_1705 0.60 -0.8 1,722,322 + +0.4 1,722,323 - +0.1 1,722,323 - -0.3 1,722,396 + -0.4 1,722,416 + +0.6 1,722,545 + -0.4 1,722,546 - -0.4 1,722,546 - +0.6 1,722,751 - EX28DRAFT_1706 0.20 -0.3 1,722,817 - EX28DRAFT_1706 0.26 -0.3 1,722,928 + EX28DRAFT_1706 0.37 -0.2 1,722,929 - EX28DRAFT_1706 0.37 -2.4 1,723,197 + EX28DRAFT_1706 0.63 -0.8 1,723,198 - EX28DRAFT_1706 0.63 -0.5 1,723,232 + EX28DRAFT_1706 0.66 +1.6 1,723,233 - EX28DRAFT_1706 0.66 -0.2 1,723,257 + EX28DRAFT_1706 0.68 -0.2 1,723,257 + EX28DRAFT_1706 0.68 -0.7 1,723,271 + EX28DRAFT_1706 0.70 -0.0
Or see this region's nucleotide sequence