Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1664 and EX28DRAFT_1665 are separated by 28 nucleotides EX28DRAFT_1665 and EX28DRAFT_1666 are separated by 72 nucleotides EX28DRAFT_1666 and EX28DRAFT_1667 are separated by 125 nucleotides
EX28DRAFT_1664: EX28DRAFT_1664 - Uncharacterized protein conserved in bacteria, at 1,678,542 to 1,680,137
_1664
EX28DRAFT_1665: EX28DRAFT_1665 - Carbonic anhydrase, at 1,680,166 to 1,680,801
_1665
EX28DRAFT_1666: EX28DRAFT_1666 - hypothetical protein, at 1,680,874 to 1,681,656
_1666
EX28DRAFT_1667: EX28DRAFT_1667 - hypothetical protein, at 1,681,782 to 1,681,922
_1667
Position (kb)
1680
1681 Strain fitness (log2 ratio)
-2
-1
0
1 at 1679.201 kb on + strand, within EX28DRAFT_1664 at 1679.202 kb on - strand, within EX28DRAFT_1664 at 1679.577 kb on + strand, within EX28DRAFT_1664 at 1679.578 kb on - strand, within EX28DRAFT_1664 at 1679.579 kb on + strand, within EX28DRAFT_1664 at 1679.686 kb on - strand, within EX28DRAFT_1664 at 1679.686 kb on - strand, within EX28DRAFT_1664 at 1679.804 kb on - strand, within EX28DRAFT_1664 at 1679.804 kb on - strand, within EX28DRAFT_1664 at 1679.952 kb on + strand, within EX28DRAFT_1664 at 1679.952 kb on + strand, within EX28DRAFT_1664 at 1679.953 kb on - strand, within EX28DRAFT_1664 at 1679.960 kb on - strand, within EX28DRAFT_1664 at 1680.168 kb on - strand at 1680.429 kb on - strand, within EX28DRAFT_1665 at 1680.817 kb on - strand at 1680.817 kb on - strand at 1680.862 kb on - strand at 1680.875 kb on + strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.876 kb on - strand at 1680.891 kb on + strand at 1680.891 kb on + strand at 1680.892 kb on - strand at 1680.892 kb on - strand at 1680.893 kb on + strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1680.894 kb on - strand at 1681.043 kb on + strand, within EX28DRAFT_1666 at 1681.053 kb on + strand, within EX28DRAFT_1666 at 1681.054 kb on - strand, within EX28DRAFT_1666 at 1681.109 kb on - strand, within EX28DRAFT_1666 at 1681.313 kb on + strand, within EX28DRAFT_1666 at 1681.314 kb on - strand, within EX28DRAFT_1666 at 1681.314 kb on - strand, within EX28DRAFT_1666 at 1681.314 kb on - strand, within EX28DRAFT_1666 at 1681.316 kb on + strand, within EX28DRAFT_1666 at 1681.317 kb on - strand, within EX28DRAFT_1666 at 1681.317 kb on - strand, within EX28DRAFT_1666 at 1681.518 kb on + strand, within EX28DRAFT_1666 at 1681.575 kb on - strand, within EX28DRAFT_1666 at 1681.622 kb on + strand at 1681.623 kb on - strand at 1681.641 kb on + strand at 1681.651 kb on - strand at 1681.685 kb on + strand at 1681.686 kb on - strand at 1681.686 kb on - strand at 1681.703 kb on + strand at 1681.704 kb on - strand at 1681.704 kb on - strand at 1681.740 kb on + strand at 1681.740 kb on + strand at 1681.740 kb on + strand at 1681.741 kb on - strand at 1681.742 kb on + strand at 1681.742 kb on + strand at 1681.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,679,201 + EX28DRAFT_1664 0.41 -1.0 1,679,202 - EX28DRAFT_1664 0.41 +0.1 1,679,577 + EX28DRAFT_1664 0.65 +0.3 1,679,578 - EX28DRAFT_1664 0.65 +1.0 1,679,579 + EX28DRAFT_1664 0.65 -0.5 1,679,686 - EX28DRAFT_1664 0.72 +0.3 1,679,686 - EX28DRAFT_1664 0.72 +0.3 1,679,804 - EX28DRAFT_1664 0.79 +0.2 1,679,804 - EX28DRAFT_1664 0.79 +0.0 1,679,952 + EX28DRAFT_1664 0.88 +0.6 1,679,952 + EX28DRAFT_1664 0.88 +0.2 1,679,953 - EX28DRAFT_1664 0.88 -0.0 1,679,960 - EX28DRAFT_1664 0.89 +1.0 1,680,168 - -0.3 1,680,429 - EX28DRAFT_1665 0.41 -1.0 1,680,817 - -0.5 1,680,817 - -0.4 1,680,862 - +0.5 1,680,875 + +0.0 1,680,876 - -0.7 1,680,876 - -2.7 1,680,876 - +0.0 1,680,891 + +0.3 1,680,891 + +0.1 1,680,892 - -0.8 1,680,892 - +0.3 1,680,893 + +0.6 1,680,894 - -0.7 1,680,894 - +0.2 1,680,894 - +0.0 1,681,043 + EX28DRAFT_1666 0.22 -0.6 1,681,053 + EX28DRAFT_1666 0.23 +0.4 1,681,054 - EX28DRAFT_1666 0.23 +0.8 1,681,109 - EX28DRAFT_1666 0.30 +0.6 1,681,313 + EX28DRAFT_1666 0.56 -1.1 1,681,314 - EX28DRAFT_1666 0.56 -0.5 1,681,314 - EX28DRAFT_1666 0.56 -0.8 1,681,314 - EX28DRAFT_1666 0.56 -1.1 1,681,316 + EX28DRAFT_1666 0.56 -0.5 1,681,317 - EX28DRAFT_1666 0.57 +0.2 1,681,317 - EX28DRAFT_1666 0.57 -0.6 1,681,518 + EX28DRAFT_1666 0.82 +0.6 1,681,575 - EX28DRAFT_1666 0.90 -0.7 1,681,622 + -1.2 1,681,623 - -0.4 1,681,641 + -0.4 1,681,651 - +0.2 1,681,685 + -0.8 1,681,686 - -0.2 1,681,686 - -2.4 1,681,703 + -0.0 1,681,704 - -0.0 1,681,704 - +0.6 1,681,740 + -0.2 1,681,740 + -0.9 1,681,740 + +0.4 1,681,741 - -1.4 1,681,742 + -0.2 1,681,742 + -1.0 1,681,743 - -1.4
Or see this region's nucleotide sequence