Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1528 and EX28DRAFT_1529 overlap by 17 nucleotides EX28DRAFT_1529 and EX28DRAFT_1530 are separated by 287 nucleotides EX28DRAFT_1530 and EX28DRAFT_1531 are separated by 45 nucleotides
EX28DRAFT_1528: EX28DRAFT_1528 - Nucleotidyltransferase/DNA polymerase involved in DNA repair, at 1,543,420 to 1,544,682
_1528
EX28DRAFT_1529: EX28DRAFT_1529 - Peptidase S24-like, at 1,544,666 to 1,545,100
_1529
EX28DRAFT_1530: EX28DRAFT_1530 - hypothetical protein, at 1,545,388 to 1,545,588
_1530
EX28DRAFT_1531: EX28DRAFT_1531 - Predicted oxidoreductases (related to aryl-alcohol dehydrogenases), at 1,545,634 to 1,546,500
_1531
Position (kb)
1545
1546 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1544.391 kb on - strand, within EX28DRAFT_1528 at 1544.454 kb on - strand, within EX28DRAFT_1528 at 1544.519 kb on + strand, within EX28DRAFT_1528 at 1544.658 kb on + strand at 1544.686 kb on + strand at 1544.686 kb on + strand at 1544.687 kb on - strand at 1544.804 kb on + strand, within EX28DRAFT_1529 at 1545.003 kb on + strand, within EX28DRAFT_1529 at 1545.003 kb on + strand, within EX28DRAFT_1529 at 1545.003 kb on + strand, within EX28DRAFT_1529 at 1545.004 kb on - strand, within EX28DRAFT_1529 at 1545.004 kb on - strand, within EX28DRAFT_1529 at 1545.126 kb on + strand at 1545.216 kb on - strand at 1545.258 kb on + strand at 1545.301 kb on + strand at 1545.491 kb on - strand, within EX28DRAFT_1530 at 1545.519 kb on + strand, within EX28DRAFT_1530 at 1545.630 kb on - strand at 1545.630 kb on - strand at 1545.846 kb on + strand, within EX28DRAFT_1531 at 1545.875 kb on + strand, within EX28DRAFT_1531 at 1545.876 kb on - strand, within EX28DRAFT_1531 at 1545.876 kb on - strand, within EX28DRAFT_1531 at 1545.938 kb on + strand, within EX28DRAFT_1531 at 1545.938 kb on + strand, within EX28DRAFT_1531 at 1545.938 kb on + strand, within EX28DRAFT_1531 at 1545.938 kb on + strand, within EX28DRAFT_1531 at 1545.938 kb on + strand, within EX28DRAFT_1531 at 1545.939 kb on - strand, within EX28DRAFT_1531 at 1545.939 kb on - strand, within EX28DRAFT_1531 at 1546.042 kb on + strand, within EX28DRAFT_1531 at 1546.327 kb on - strand, within EX28DRAFT_1531 at 1546.436 kb on + strand at 1546.473 kb on - strand at 1546.486 kb on - strand at 1546.558 kb on + strand at 1546.574 kb on - strand at 1546.577 kb on + strand at 1546.578 kb on - strand at 1546.578 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,544,391 - EX28DRAFT_1528 0.77 +2.0 1,544,454 - EX28DRAFT_1528 0.82 -0.4 1,544,519 + EX28DRAFT_1528 0.87 +0.4 1,544,658 + -0.0 1,544,686 + -0.4 1,544,686 + +0.2 1,544,687 - -2.3 1,544,804 + EX28DRAFT_1529 0.32 +0.5 1,545,003 + EX28DRAFT_1529 0.77 -1.9 1,545,003 + EX28DRAFT_1529 0.77 -0.2 1,545,003 + EX28DRAFT_1529 0.77 -0.4 1,545,004 - EX28DRAFT_1529 0.78 +0.0 1,545,004 - EX28DRAFT_1529 0.78 -0.0 1,545,126 + -0.1 1,545,216 - -1.3 1,545,258 + -1.5 1,545,301 + +0.3 1,545,491 - EX28DRAFT_1530 0.51 -2.2 1,545,519 + EX28DRAFT_1530 0.65 -0.8 1,545,630 - -0.3 1,545,630 - +0.9 1,545,846 + EX28DRAFT_1531 0.24 -2.8 1,545,875 + EX28DRAFT_1531 0.28 +0.1 1,545,876 - EX28DRAFT_1531 0.28 +0.4 1,545,876 - EX28DRAFT_1531 0.28 +0.0 1,545,938 + EX28DRAFT_1531 0.35 -1.0 1,545,938 + EX28DRAFT_1531 0.35 -0.7 1,545,938 + EX28DRAFT_1531 0.35 +0.7 1,545,938 + EX28DRAFT_1531 0.35 -0.2 1,545,938 + EX28DRAFT_1531 0.35 -0.1 1,545,939 - EX28DRAFT_1531 0.35 -0.0 1,545,939 - EX28DRAFT_1531 0.35 -0.0 1,546,042 + EX28DRAFT_1531 0.47 +0.2 1,546,327 - EX28DRAFT_1531 0.80 -0.1 1,546,436 + -0.0 1,546,473 - +0.7 1,546,486 - +0.5 1,546,558 + +0.3 1,546,574 - -1.0 1,546,577 + -2.0 1,546,578 - -0.5 1,546,578 - +0.0
Or see this region's nucleotide sequence