Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1486 and EX28DRAFT_1487 are separated by 276 nucleotides EX28DRAFT_1487 and EX28DRAFT_1488 are separated by 18 nucleotides EX28DRAFT_1488 and EX28DRAFT_1489 are separated by 8 nucleotides
EX28DRAFT_1486: EX28DRAFT_1486 - Predicted membrane protein, at 1,500,451 to 1,501,242
_1486
EX28DRAFT_1487: EX28DRAFT_1487 - ABC-type proline/glycine betaine transport systems, permease component, at 1,501,519 to 1,502,229
_1487
EX28DRAFT_1488: EX28DRAFT_1488 - Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein), at 1,502,248 to 1,503,147
_1488
EX28DRAFT_1489: EX28DRAFT_1489 - ABC-type proline/glycine betaine transport systems, permease component, at 1,503,156 to 1,503,803
_1489
Position (kb)
1501
1502
1503 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1500.538 kb on - strand, within EX28DRAFT_1486 at 1500.691 kb on - strand, within EX28DRAFT_1486 at 1500.752 kb on + strand, within EX28DRAFT_1486 at 1500.752 kb on + strand, within EX28DRAFT_1486 at 1500.752 kb on + strand, within EX28DRAFT_1486 at 1500.752 kb on + strand, within EX28DRAFT_1486 at 1500.753 kb on - strand, within EX28DRAFT_1486 at 1500.838 kb on + strand, within EX28DRAFT_1486 at 1500.839 kb on - strand, within EX28DRAFT_1486 at 1500.839 kb on - strand, within EX28DRAFT_1486 at 1500.847 kb on + strand, within EX28DRAFT_1486 at 1500.848 kb on - strand, within EX28DRAFT_1486 at 1500.990 kb on - strand, within EX28DRAFT_1486 at 1501.030 kb on + strand, within EX28DRAFT_1486 at 1501.030 kb on + strand, within EX28DRAFT_1486 at 1501.077 kb on - strand, within EX28DRAFT_1486 at 1501.238 kb on + strand at 1501.247 kb on - strand at 1501.310 kb on + strand at 1501.343 kb on + strand at 1501.443 kb on - strand at 1501.443 kb on - strand at 1501.490 kb on - strand at 1501.530 kb on - strand at 1501.564 kb on - strand at 1501.636 kb on + strand, within EX28DRAFT_1487 at 1501.750 kb on + strand, within EX28DRAFT_1487 at 1501.751 kb on - strand, within EX28DRAFT_1487 at 1501.895 kb on - strand, within EX28DRAFT_1487 at 1502.233 kb on + strand at 1502.247 kb on + strand at 1502.248 kb on - strand at 1502.483 kb on - strand, within EX28DRAFT_1488 at 1502.613 kb on + strand, within EX28DRAFT_1488 at 1502.613 kb on + strand, within EX28DRAFT_1488 at 1502.613 kb on + strand, within EX28DRAFT_1488 at 1502.613 kb on + strand, within EX28DRAFT_1488 at 1502.614 kb on - strand, within EX28DRAFT_1488 at 1502.659 kb on - strand, within EX28DRAFT_1488 at 1502.703 kb on + strand, within EX28DRAFT_1488 at 1502.845 kb on + strand, within EX28DRAFT_1488 at 1502.846 kb on - strand, within EX28DRAFT_1488 at 1502.848 kb on - strand, within EX28DRAFT_1488 at 1502.887 kb on + strand, within EX28DRAFT_1488 at 1503.171 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,500,538 - EX28DRAFT_1486 0.11 -0.8 1,500,691 - EX28DRAFT_1486 0.30 +0.6 1,500,752 + EX28DRAFT_1486 0.38 +0.3 1,500,752 + EX28DRAFT_1486 0.38 -0.1 1,500,752 + EX28DRAFT_1486 0.38 -0.7 1,500,752 + EX28DRAFT_1486 0.38 +0.5 1,500,753 - EX28DRAFT_1486 0.38 +0.0 1,500,838 + EX28DRAFT_1486 0.49 -0.7 1,500,839 - EX28DRAFT_1486 0.49 -0.3 1,500,839 - EX28DRAFT_1486 0.49 -0.2 1,500,847 + EX28DRAFT_1486 0.50 -0.6 1,500,848 - EX28DRAFT_1486 0.50 +0.2 1,500,990 - EX28DRAFT_1486 0.68 -0.2 1,501,030 + EX28DRAFT_1486 0.73 +0.9 1,501,030 + EX28DRAFT_1486 0.73 -0.2 1,501,077 - EX28DRAFT_1486 0.79 +0.5 1,501,238 + +1.3 1,501,247 - -0.2 1,501,310 + +0.3 1,501,343 + +0.2 1,501,443 - -0.4 1,501,443 - +0.3 1,501,490 - +2.3 1,501,530 - +0.2 1,501,564 - -0.0 1,501,636 + EX28DRAFT_1487 0.16 +0.1 1,501,750 + EX28DRAFT_1487 0.32 +0.5 1,501,751 - EX28DRAFT_1487 0.33 -0.2 1,501,895 - EX28DRAFT_1487 0.53 +1.8 1,502,233 + -0.9 1,502,247 + -0.0 1,502,248 - -1.1 1,502,483 - EX28DRAFT_1488 0.26 -0.0 1,502,613 + EX28DRAFT_1488 0.41 -0.8 1,502,613 + EX28DRAFT_1488 0.41 +0.2 1,502,613 + EX28DRAFT_1488 0.41 +1.8 1,502,613 + EX28DRAFT_1488 0.41 -0.1 1,502,614 - EX28DRAFT_1488 0.41 +0.2 1,502,659 - EX28DRAFT_1488 0.46 +0.4 1,502,703 + EX28DRAFT_1488 0.51 -1.5 1,502,845 + EX28DRAFT_1488 0.66 +0.2 1,502,846 - EX28DRAFT_1488 0.66 -2.1 1,502,848 - EX28DRAFT_1488 0.67 +0.4 1,502,887 + EX28DRAFT_1488 0.71 +0.6 1,503,171 + +0.3
Or see this region's nucleotide sequence