Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1324 and EX28DRAFT_1325 overlap by 4 nucleotides EX28DRAFT_1325 and EX28DRAFT_1326 are separated by 118 nucleotides EX28DRAFT_1326 and EX28DRAFT_1327 are separated by 1 nucleotides EX28DRAFT_1327 and EX28DRAFT_1328 are separated by 143 nucleotides EX28DRAFT_1328 and EX28DRAFT_1329 overlap by 1 nucleotides
EX28DRAFT_1324: EX28DRAFT_1324 - Methyltransferase domain, at 1,322,742 to 1,323,224
_1324
EX28DRAFT_1325: EX28DRAFT_1325 - Transcriptional activator, adenine-specific DNA methyltransferase, at 1,323,221 to 1,323,880
_1325
EX28DRAFT_1326: EX28DRAFT_1326 - transcriptional regulator, TraR/DksA family, at 1,323,999 to 1,324,214
_1326
EX28DRAFT_1327: EX28DRAFT_1327 - hypothetical protein, at 1,324,216 to 1,324,740
_1327
EX28DRAFT_1328: EX28DRAFT_1328 - Putative excisionase (DUF1233), at 1,324,884 to 1,325,096
_1328
EX28DRAFT_1329: EX28DRAFT_1329 - Site-specific recombinase XerD, at 1,325,096 to 1,326,376
_1329
Position (kb) 1323
1324
1325 Strain fitness (log2 ratio) -2
-1
0
1 at 1323.125 kb on + strand, within EX28DRAFT_1324 at 1323.158 kb on - strand, within EX28DRAFT_1324 at 1323.241 kb on + strand at 1323.241 kb on + strand at 1323.245 kb on + strand at 1323.246 kb on - strand at 1323.267 kb on - strand at 1323.386 kb on + strand, within EX28DRAFT_1325 at 1323.665 kb on - strand, within EX28DRAFT_1325 at 1323.672 kb on + strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.676 kb on - strand, within EX28DRAFT_1325 at 1323.677 kb on + strand, within EX28DRAFT_1325 at 1323.678 kb on - strand, within EX28DRAFT_1325 at 1323.678 kb on - strand, within EX28DRAFT_1325 at 1323.678 kb on - strand, within EX28DRAFT_1325 at 1323.915 kb on - strand at 1323.947 kb on + strand at 1323.973 kb on - strand at 1324.213 kb on - strand at 1324.236 kb on - strand at 1324.302 kb on - strand, within EX28DRAFT_1327 at 1324.323 kb on - strand, within EX28DRAFT_1327 at 1324.340 kb on - strand, within EX28DRAFT_1327 at 1324.347 kb on - strand, within EX28DRAFT_1327 at 1324.363 kb on - strand, within EX28DRAFT_1327 at 1324.405 kb on + strand, within EX28DRAFT_1327 at 1324.422 kb on - strand, within EX28DRAFT_1327 at 1324.422 kb on - strand, within EX28DRAFT_1327 at 1324.440 kb on + strand, within EX28DRAFT_1327 at 1324.440 kb on + strand, within EX28DRAFT_1327 at 1324.441 kb on - strand, within EX28DRAFT_1327 at 1324.474 kb on - strand, within EX28DRAFT_1327 at 1324.479 kb on + strand, within EX28DRAFT_1327 at 1324.480 kb on - strand, within EX28DRAFT_1327 at 1324.480 kb on - strand, within EX28DRAFT_1327 at 1324.484 kb on - strand, within EX28DRAFT_1327 at 1324.558 kb on + strand, within EX28DRAFT_1327 at 1324.558 kb on + strand, within EX28DRAFT_1327 at 1324.621 kb on + strand, within EX28DRAFT_1327 at 1324.622 kb on - strand, within EX28DRAFT_1327 at 1324.656 kb on + strand, within EX28DRAFT_1327 at 1324.855 kb on + strand at 1324.855 kb on + strand at 1324.857 kb on + strand at 1324.857 kb on + strand at 1325.055 kb on + strand, within EX28DRAFT_1328 at 1325.062 kb on - strand, within EX28DRAFT_1328 at 1325.080 kb on + strand at 1325.094 kb on + strand at 1325.095 kb on - strand at 1325.095 kb on - strand at 1325.209 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,323,125 + EX28DRAFT_1324 0.79 +1.5 1,323,158 - EX28DRAFT_1324 0.86 +0.3 1,323,241 + +0.1 1,323,241 + +0.9 1,323,245 + +0.1 1,323,246 - -1.4 1,323,267 - -0.8 1,323,386 + EX28DRAFT_1325 0.25 -0.1 1,323,665 - EX28DRAFT_1325 0.67 -0.6 1,323,672 + EX28DRAFT_1325 0.68 -0.2 1,323,676 - EX28DRAFT_1325 0.69 +0.2 1,323,676 - EX28DRAFT_1325 0.69 -0.6 1,323,676 - EX28DRAFT_1325 0.69 -0.2 1,323,676 - EX28DRAFT_1325 0.69 +1.0 1,323,677 + EX28DRAFT_1325 0.69 -1.9 1,323,678 - EX28DRAFT_1325 0.69 -0.4 1,323,678 - EX28DRAFT_1325 0.69 +0.3 1,323,678 - EX28DRAFT_1325 0.69 +0.7 1,323,915 - +0.4 1,323,947 + -0.4 1,323,973 - +1.4 1,324,213 - -0.0 1,324,236 - -1.6 1,324,302 - EX28DRAFT_1327 0.16 +0.7 1,324,323 - EX28DRAFT_1327 0.20 +0.6 1,324,340 - EX28DRAFT_1327 0.24 -0.1 1,324,347 - EX28DRAFT_1327 0.25 +0.4 1,324,363 - EX28DRAFT_1327 0.28 -0.6 1,324,405 + EX28DRAFT_1327 0.36 +0.7 1,324,422 - EX28DRAFT_1327 0.39 -0.9 1,324,422 - EX28DRAFT_1327 0.39 -0.1 1,324,440 + EX28DRAFT_1327 0.43 -0.3 1,324,440 + EX28DRAFT_1327 0.43 -0.1 1,324,441 - EX28DRAFT_1327 0.43 +0.0 1,324,474 - EX28DRAFT_1327 0.49 +0.3 1,324,479 + EX28DRAFT_1327 0.50 +0.5 1,324,480 - EX28DRAFT_1327 0.50 +0.2 1,324,480 - EX28DRAFT_1327 0.50 +0.1 1,324,484 - EX28DRAFT_1327 0.51 +0.1 1,324,558 + EX28DRAFT_1327 0.65 +0.2 1,324,558 + EX28DRAFT_1327 0.65 -0.0 1,324,621 + EX28DRAFT_1327 0.77 -0.5 1,324,622 - EX28DRAFT_1327 0.77 +0.2 1,324,656 + EX28DRAFT_1327 0.84 +0.6 1,324,855 + -0.4 1,324,855 + -0.6 1,324,857 + +0.4 1,324,857 + -0.9 1,325,055 + EX28DRAFT_1328 0.80 +1.7 1,325,062 - EX28DRAFT_1328 0.84 -0.8 1,325,080 + +0.0 1,325,094 + -0.3 1,325,095 - +0.2 1,325,095 - -0.2 1,325,209 + +0.6
Or see this region's nucleotide sequence