Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1298 and EX28DRAFT_1299 are separated by 82 nucleotides EX28DRAFT_1299 and EX28DRAFT_1300 overlap by 4 nucleotides EX28DRAFT_1300 and EX28DRAFT_1301 overlap by 4 nucleotides EX28DRAFT_1301 and EX28DRAFT_1302 overlap by 4 nucleotides EX28DRAFT_1302 and EX28DRAFT_1303 overlap by 8 nucleotides EX28DRAFT_1303 and EX28DRAFT_1304 overlap by 8 nucleotides
EX28DRAFT_1298: EX28DRAFT_1298 - hypothetical protein, at 1,309,142 to 1,309,351
_1298
EX28DRAFT_1299: EX28DRAFT_1299 - Phage antitermination protein Q, at 1,309,434 to 1,310,123
_1299
EX28DRAFT_1300: EX28DRAFT_1300 - hypothetical protein, at 1,310,120 to 1,310,260
_1300
EX28DRAFT_1301: EX28DRAFT_1301 - hypothetical protein, at 1,310,257 to 1,310,481
_1301
EX28DRAFT_1302: EX28DRAFT_1302 - Bacteriophage Lambda NinG protein, at 1,310,478 to 1,311,089
_1302
EX28DRAFT_1303: EX28DRAFT_1303 - hypothetical protein, at 1,311,082 to 1,311,252
_1303
EX28DRAFT_1304: EX28DRAFT_1304 - NinB protein, at 1,311,245 to 1,311,694
_1304
Position (kb)
1310
1311 Strain fitness (log2 ratio)
-1
0
1 at 1309.143 kb on + strand at 1309.151 kb on + strand at 1309.151 kb on + strand at 1309.190 kb on - strand, within EX28DRAFT_1298 at 1309.308 kb on - strand, within EX28DRAFT_1298 at 1309.324 kb on + strand, within EX28DRAFT_1298 at 1309.325 kb on - strand, within EX28DRAFT_1298 at 1309.325 kb on - strand, within EX28DRAFT_1298 at 1309.343 kb on + strand at 1309.619 kb on - strand, within EX28DRAFT_1299 at 1309.676 kb on + strand, within EX28DRAFT_1299 at 1309.677 kb on - strand, within EX28DRAFT_1299 at 1309.798 kb on + strand, within EX28DRAFT_1299 at 1309.799 kb on - strand, within EX28DRAFT_1299 at 1309.799 kb on - strand, within EX28DRAFT_1299 at 1309.965 kb on - strand, within EX28DRAFT_1299 at 1310.037 kb on + strand, within EX28DRAFT_1299 at 1310.101 kb on + strand at 1310.146 kb on - strand, within EX28DRAFT_1300 at 1310.238 kb on - strand, within EX28DRAFT_1300 at 1310.285 kb on + strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.286 kb on - strand, within EX28DRAFT_1301 at 1310.294 kb on + strand, within EX28DRAFT_1301 at 1310.400 kb on + strand, within EX28DRAFT_1301 at 1310.461 kb on + strand at 1310.462 kb on - strand at 1310.462 kb on - strand at 1310.465 kb on + strand at 1310.466 kb on - strand at 1310.466 kb on - strand at 1310.475 kb on + strand at 1310.632 kb on + strand, within EX28DRAFT_1302 at 1310.687 kb on + strand, within EX28DRAFT_1302 at 1310.688 kb on - strand, within EX28DRAFT_1302 at 1310.699 kb on + strand, within EX28DRAFT_1302 at 1310.781 kb on - strand, within EX28DRAFT_1302 at 1310.781 kb on - strand, within EX28DRAFT_1302 at 1311.046 kb on + strand at 1311.069 kb on - strand at 1311.084 kb on - strand at 1311.144 kb on - strand, within EX28DRAFT_1303 at 1311.186 kb on + strand, within EX28DRAFT_1303 at 1311.246 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,309,143 + +0.1 1,309,151 + +0.3 1,309,151 + +0.3 1,309,190 - EX28DRAFT_1298 0.23 -0.0 1,309,308 - EX28DRAFT_1298 0.79 +0.3 1,309,324 + EX28DRAFT_1298 0.87 +0.0 1,309,325 - EX28DRAFT_1298 0.87 +0.2 1,309,325 - EX28DRAFT_1298 0.87 -0.3 1,309,343 + +0.4 1,309,619 - EX28DRAFT_1299 0.27 +0.2 1,309,676 + EX28DRAFT_1299 0.35 -0.2 1,309,677 - EX28DRAFT_1299 0.35 +0.1 1,309,798 + EX28DRAFT_1299 0.53 +0.2 1,309,799 - EX28DRAFT_1299 0.53 -1.2 1,309,799 - EX28DRAFT_1299 0.53 -0.1 1,309,965 - EX28DRAFT_1299 0.77 +0.0 1,310,037 + EX28DRAFT_1299 0.87 +0.0 1,310,101 + -0.2 1,310,146 - EX28DRAFT_1300 0.18 -1.6 1,310,238 - EX28DRAFT_1300 0.84 -1.1 1,310,285 + EX28DRAFT_1301 0.12 -0.3 1,310,286 - EX28DRAFT_1301 0.13 +0.3 1,310,286 - EX28DRAFT_1301 0.13 +0.3 1,310,286 - EX28DRAFT_1301 0.13 +0.3 1,310,294 + EX28DRAFT_1301 0.16 +0.6 1,310,400 + EX28DRAFT_1301 0.64 +0.3 1,310,461 + -1.3 1,310,462 - +0.7 1,310,462 - -0.1 1,310,465 + -0.7 1,310,466 - +0.7 1,310,466 - +0.2 1,310,475 + +1.3 1,310,632 + EX28DRAFT_1302 0.25 +0.1 1,310,687 + EX28DRAFT_1302 0.34 -0.3 1,310,688 - EX28DRAFT_1302 0.34 -0.3 1,310,699 + EX28DRAFT_1302 0.36 -0.1 1,310,781 - EX28DRAFT_1302 0.50 -0.1 1,310,781 - EX28DRAFT_1302 0.50 +0.3 1,311,046 + +1.1 1,311,069 - +0.3 1,311,084 - -1.5 1,311,144 - EX28DRAFT_1303 0.36 +0.1 1,311,186 + EX28DRAFT_1303 0.61 +0.7 1,311,246 + -0.0
Or see this region's nucleotide sequence