Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1215 and EX28DRAFT_1216 are separated by 203 nucleotides EX28DRAFT_1216 and EX28DRAFT_1217 are separated by 156 nucleotides EX28DRAFT_1217 and EX28DRAFT_1218 are separated by 61 nucleotides
EX28DRAFT_1215: EX28DRAFT_1215 - urea ABC transporter, urea binding protein, at 1,233,466 to 1,234,737
_1215
EX28DRAFT_1216: EX28DRAFT_1216 - Methionine synthase II (cobalamin-independent), at 1,234,941 to 1,236,044
_1216
EX28DRAFT_1217: EX28DRAFT_1217 - Rhodanese-related sulfurtransferase, at 1,236,201 to 1,236,614
_1217
EX28DRAFT_1218: EX28DRAFT_1218 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 1,236,676 to 1,237,695
_1218
Position (kb)
1234
1235
1236
1237 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1234.011 kb on - strand, within EX28DRAFT_1215 at 1234.127 kb on + strand, within EX28DRAFT_1215 at 1234.127 kb on + strand, within EX28DRAFT_1215 at 1234.205 kb on + strand, within EX28DRAFT_1215 at 1234.212 kb on - strand, within EX28DRAFT_1215 at 1234.313 kb on + strand, within EX28DRAFT_1215 at 1234.313 kb on + strand, within EX28DRAFT_1215 at 1234.314 kb on - strand, within EX28DRAFT_1215 at 1234.514 kb on - strand, within EX28DRAFT_1215 at 1234.640 kb on - strand at 1234.720 kb on + strand at 1234.720 kb on + strand at 1234.725 kb on + strand at 1234.725 kb on + strand at 1234.725 kb on + strand at 1234.726 kb on - strand at 1234.845 kb on - strand at 1234.850 kb on + strand at 1234.877 kb on + strand at 1234.878 kb on - strand at 1234.878 kb on - strand at 1234.878 kb on - strand at 1234.917 kb on + strand at 1235.074 kb on + strand, within EX28DRAFT_1216 at 1235.102 kb on + strand, within EX28DRAFT_1216 at 1235.222 kb on - strand, within EX28DRAFT_1216 at 1235.389 kb on + strand, within EX28DRAFT_1216 at 1235.389 kb on + strand, within EX28DRAFT_1216 at 1235.557 kb on + strand, within EX28DRAFT_1216 at 1235.558 kb on - strand, within EX28DRAFT_1216 at 1236.123 kb on - strand at 1236.123 kb on - strand at 1236.144 kb on + strand at 1236.352 kb on + strand, within EX28DRAFT_1217 at 1236.436 kb on - strand, within EX28DRAFT_1217 at 1236.478 kb on - strand, within EX28DRAFT_1217 at 1236.602 kb on + strand at 1236.607 kb on + strand at 1236.649 kb on + strand at 1236.649 kb on + strand at 1236.649 kb on + strand at 1236.650 kb on - strand at 1236.650 kb on - strand at 1236.771 kb on + strand at 1236.918 kb on - strand, within EX28DRAFT_1218
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,234,011 - EX28DRAFT_1215 0.43 -1.4 1,234,127 + EX28DRAFT_1215 0.52 +0.1 1,234,127 + EX28DRAFT_1215 0.52 -0.0 1,234,205 + EX28DRAFT_1215 0.58 -3.2 1,234,212 - EX28DRAFT_1215 0.59 -0.8 1,234,313 + EX28DRAFT_1215 0.67 +2.5 1,234,313 + EX28DRAFT_1215 0.67 +0.1 1,234,314 - EX28DRAFT_1215 0.67 +0.2 1,234,514 - EX28DRAFT_1215 0.82 -1.0 1,234,640 - -0.5 1,234,720 + -2.8 1,234,720 + -0.6 1,234,725 + -0.6 1,234,725 + -0.4 1,234,725 + +0.5 1,234,726 - -1.4 1,234,845 - +0.0 1,234,850 + -0.6 1,234,877 + +0.4 1,234,878 - -0.4 1,234,878 - +0.4 1,234,878 - -0.6 1,234,917 + -0.4 1,235,074 + EX28DRAFT_1216 0.12 +0.4 1,235,102 + EX28DRAFT_1216 0.15 -0.7 1,235,222 - EX28DRAFT_1216 0.25 -0.3 1,235,389 + EX28DRAFT_1216 0.41 +0.4 1,235,389 + EX28DRAFT_1216 0.41 -0.2 1,235,557 + EX28DRAFT_1216 0.56 +0.0 1,235,558 - EX28DRAFT_1216 0.56 -0.3 1,236,123 - -1.2 1,236,123 - +0.3 1,236,144 + -2.4 1,236,352 + EX28DRAFT_1217 0.36 -0.1 1,236,436 - EX28DRAFT_1217 0.57 +1.5 1,236,478 - EX28DRAFT_1217 0.67 -0.2 1,236,602 + +0.4 1,236,607 + -0.0 1,236,649 + -2.0 1,236,649 + -0.4 1,236,649 + +1.2 1,236,650 - +0.1 1,236,650 - +1.1 1,236,771 + -0.5 1,236,918 - EX28DRAFT_1218 0.24 -0.5
Or see this region's nucleotide sequence