Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1183 and EX28DRAFT_1184 are separated by 135 nucleotides EX28DRAFT_1184 and EX28DRAFT_1185 overlap by 4 nucleotides EX28DRAFT_1185 and EX28DRAFT_1186 are separated by 44 nucleotides
EX28DRAFT_1183: EX28DRAFT_1183 - Cytochrome c biogenesis protein, at 1,203,150 to 1,204,340
_1183
EX28DRAFT_1184: EX28DRAFT_1184 - Signal transduction histidine kinase, at 1,204,476 to 1,205,774
_1184
EX28DRAFT_1185: EX28DRAFT_1185 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 1,205,771 to 1,206,511
_1185
EX28DRAFT_1186: EX28DRAFT_1186 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 1,206,556 to 1,207,551
_1186
Position (kb)
1204
1205
1206 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1203.729 kb on + strand, within EX28DRAFT_1183 at 1203.729 kb on + strand, within EX28DRAFT_1183 at 1203.730 kb on - strand, within EX28DRAFT_1183 at 1203.730 kb on - strand, within EX28DRAFT_1183 at 1203.825 kb on + strand, within EX28DRAFT_1183 at 1203.915 kb on - strand, within EX28DRAFT_1183 at 1204.032 kb on + strand, within EX28DRAFT_1183 at 1204.032 kb on + strand, within EX28DRAFT_1183 at 1204.032 kb on + strand, within EX28DRAFT_1183 at 1204.033 kb on - strand, within EX28DRAFT_1183 at 1204.033 kb on - strand, within EX28DRAFT_1183 at 1204.033 kb on - strand, within EX28DRAFT_1183 at 1204.034 kb on + strand, within EX28DRAFT_1183 at 1204.035 kb on - strand, within EX28DRAFT_1183 at 1204.065 kb on - strand, within EX28DRAFT_1183 at 1204.286 kb on + strand at 1204.287 kb on - strand at 1204.306 kb on - strand at 1204.329 kb on + strand at 1204.340 kb on + strand at 1204.340 kb on + strand at 1204.354 kb on + strand at 1204.354 kb on + strand at 1204.355 kb on - strand at 1204.355 kb on - strand at 1204.383 kb on - strand at 1204.409 kb on + strand at 1204.410 kb on - strand at 1204.461 kb on + strand at 1204.462 kb on - strand at 1204.462 kb on - strand at 1204.462 kb on - strand at 1204.642 kb on - strand, within EX28DRAFT_1184 at 1204.642 kb on - strand, within EX28DRAFT_1184 at 1204.755 kb on - strand, within EX28DRAFT_1184 at 1204.755 kb on - strand, within EX28DRAFT_1184 at 1204.819 kb on - strand, within EX28DRAFT_1184 at 1204.949 kb on - strand, within EX28DRAFT_1184 at 1205.113 kb on + strand, within EX28DRAFT_1184 at 1205.114 kb on - strand, within EX28DRAFT_1184 at 1205.444 kb on - strand, within EX28DRAFT_1184 at 1205.536 kb on + strand, within EX28DRAFT_1184 at 1205.806 kb on + strand at 1205.814 kb on + strand at 1205.839 kb on + strand at 1205.841 kb on + strand at 1206.050 kb on + strand, within EX28DRAFT_1185 at 1206.204 kb on + strand, within EX28DRAFT_1185 at 1206.204 kb on + strand, within EX28DRAFT_1185 at 1206.204 kb on + strand, within EX28DRAFT_1185 at 1206.205 kb on - strand, within EX28DRAFT_1185 at 1206.205 kb on - strand, within EX28DRAFT_1185 at 1206.205 kb on - strand, within EX28DRAFT_1185 at 1206.205 kb on - strand, within EX28DRAFT_1185 at 1206.288 kb on + strand, within EX28DRAFT_1185 at 1206.288 kb on + strand, within EX28DRAFT_1185 at 1206.289 kb on - strand, within EX28DRAFT_1185 at 1206.289 kb on - strand, within EX28DRAFT_1185 at 1206.303 kb on - strand, within EX28DRAFT_1185 at 1206.320 kb on - strand, within EX28DRAFT_1185 at 1206.367 kb on + strand, within EX28DRAFT_1185 at 1206.436 kb on + strand, within EX28DRAFT_1185 at 1206.436 kb on + strand, within EX28DRAFT_1185 at 1206.493 kb on + strand at 1206.502 kb on + strand at 1206.502 kb on + strand at 1206.502 kb on + strand at 1206.547 kb on + strand at 1206.547 kb on + strand at 1206.548 kb on - strand at 1206.653 kb on + strand at 1206.773 kb on + strand, within EX28DRAFT_1186
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,203,729 + EX28DRAFT_1183 0.49 -0.2 1,203,729 + EX28DRAFT_1183 0.49 +0.4 1,203,730 - EX28DRAFT_1183 0.49 +1.0 1,203,730 - EX28DRAFT_1183 0.49 -0.6 1,203,825 + EX28DRAFT_1183 0.57 -0.7 1,203,915 - EX28DRAFT_1183 0.64 +0.5 1,204,032 + EX28DRAFT_1183 0.74 -0.5 1,204,032 + EX28DRAFT_1183 0.74 +0.5 1,204,032 + EX28DRAFT_1183 0.74 -1.1 1,204,033 - EX28DRAFT_1183 0.74 +0.6 1,204,033 - EX28DRAFT_1183 0.74 +0.3 1,204,033 - EX28DRAFT_1183 0.74 -0.4 1,204,034 + EX28DRAFT_1183 0.74 +0.1 1,204,035 - EX28DRAFT_1183 0.74 +0.7 1,204,065 - EX28DRAFT_1183 0.77 +0.0 1,204,286 + -0.6 1,204,287 - -0.8 1,204,306 - +0.1 1,204,329 + -0.3 1,204,340 + +0.7 1,204,340 + -0.5 1,204,354 + +0.1 1,204,354 + +0.6 1,204,355 - -0.2 1,204,355 - -0.7 1,204,383 - +0.4 1,204,409 + +1.4 1,204,410 - -3.2 1,204,461 + -0.6 1,204,462 - -1.0 1,204,462 - -0.1 1,204,462 - +0.1 1,204,642 - EX28DRAFT_1184 0.13 -0.4 1,204,642 - EX28DRAFT_1184 0.13 -0.4 1,204,755 - EX28DRAFT_1184 0.21 -0.0 1,204,755 - EX28DRAFT_1184 0.21 -3.2 1,204,819 - EX28DRAFT_1184 0.26 +1.8 1,204,949 - EX28DRAFT_1184 0.36 -0.3 1,205,113 + EX28DRAFT_1184 0.49 +0.2 1,205,114 - EX28DRAFT_1184 0.49 -0.5 1,205,444 - EX28DRAFT_1184 0.75 +0.2 1,205,536 + EX28DRAFT_1184 0.82 -1.2 1,205,806 + +0.1 1,205,814 + +0.0 1,205,839 + -0.0 1,205,841 + +0.4 1,206,050 + EX28DRAFT_1185 0.38 -0.3 1,206,204 + EX28DRAFT_1185 0.58 +0.3 1,206,204 + EX28DRAFT_1185 0.58 +0.3 1,206,204 + EX28DRAFT_1185 0.58 -1.0 1,206,205 - EX28DRAFT_1185 0.59 -0.4 1,206,205 - EX28DRAFT_1185 0.59 -0.1 1,206,205 - EX28DRAFT_1185 0.59 +0.6 1,206,205 - EX28DRAFT_1185 0.59 -0.2 1,206,288 + EX28DRAFT_1185 0.70 -0.2 1,206,288 + EX28DRAFT_1185 0.70 +0.2 1,206,289 - EX28DRAFT_1185 0.70 +0.6 1,206,289 - EX28DRAFT_1185 0.70 -0.5 1,206,303 - EX28DRAFT_1185 0.72 -0.8 1,206,320 - EX28DRAFT_1185 0.74 +0.4 1,206,367 + EX28DRAFT_1185 0.80 +0.4 1,206,436 + EX28DRAFT_1185 0.90 +1.9 1,206,436 + EX28DRAFT_1185 0.90 +0.2 1,206,493 + -0.9 1,206,502 + -1.6 1,206,502 + +0.4 1,206,502 + +0.0 1,206,547 + +1.8 1,206,547 + -0.4 1,206,548 - +1.0 1,206,653 + +0.3 1,206,773 + EX28DRAFT_1186 0.22 -0.7
Or see this region's nucleotide sequence