Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1173 and EX28DRAFT_1174 are separated by 75 nucleotides EX28DRAFT_1174 and EX28DRAFT_1175 are separated by 38 nucleotides EX28DRAFT_1175 and EX28DRAFT_1176 are separated by 100 nucleotides
EX28DRAFT_1173: EX28DRAFT_1173 - Nucleoside-diphosphate-sugar epimerases, at 1,195,952 to 1,196,914
_1173
EX28DRAFT_1174: EX28DRAFT_1174 - Predicted membrane protein, at 1,196,990 to 1,197,433
_1174
EX28DRAFT_1175: EX28DRAFT_1175 - Domain of unknown function (DUF4354), at 1,197,472 to 1,197,900
_1175
EX28DRAFT_1176: EX28DRAFT_1176 - Predicted lactoylglutathione lyase, at 1,198,001 to 1,198,360
_1176
Position (kb)
1196
1197
1198 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1196.227 kb on + strand, within EX28DRAFT_1173 at 1196.263 kb on - strand, within EX28DRAFT_1173 at 1196.285 kb on + strand, within EX28DRAFT_1173 at 1196.286 kb on - strand, within EX28DRAFT_1173 at 1196.287 kb on + strand, within EX28DRAFT_1173 at 1196.288 kb on - strand, within EX28DRAFT_1173 at 1196.291 kb on + strand, within EX28DRAFT_1173 at 1196.292 kb on - strand, within EX28DRAFT_1173 at 1196.317 kb on - strand, within EX28DRAFT_1173 at 1196.362 kb on - strand, within EX28DRAFT_1173 at 1196.394 kb on - strand, within EX28DRAFT_1173 at 1196.394 kb on - strand, within EX28DRAFT_1173 at 1196.484 kb on + strand, within EX28DRAFT_1173 at 1196.676 kb on + strand, within EX28DRAFT_1173 at 1196.705 kb on - strand, within EX28DRAFT_1173 at 1196.811 kb on + strand, within EX28DRAFT_1173 at 1196.845 kb on - strand at 1196.888 kb on - strand at 1196.888 kb on - strand at 1196.912 kb on + strand at 1196.913 kb on - strand at 1196.918 kb on - strand at 1196.948 kb on - strand at 1196.954 kb on + strand at 1196.957 kb on - strand at 1197.361 kb on + strand, within EX28DRAFT_1174 at 1197.361 kb on + strand, within EX28DRAFT_1174 at 1197.384 kb on - strand, within EX28DRAFT_1174 at 1197.429 kb on + strand at 1197.431 kb on + strand at 1197.431 kb on + strand at 1197.431 kb on + strand at 1197.431 kb on + strand at 1197.431 kb on + strand at 1197.432 kb on - strand at 1197.432 kb on - strand at 1197.432 kb on - strand at 1197.435 kb on + strand at 1197.435 kb on + strand at 1197.436 kb on - strand at 1197.436 kb on - strand at 1197.436 kb on - strand at 1197.436 kb on - strand at 1197.436 kb on - strand at 1197.437 kb on + strand at 1197.438 kb on - strand at 1197.438 kb on - strand at 1197.438 kb on - strand at 1197.438 kb on - strand at 1197.438 kb on - strand at 1197.438 kb on - strand at 1197.440 kb on + strand at 1197.440 kb on + strand at 1197.643 kb on + strand, within EX28DRAFT_1175 at 1197.646 kb on - strand, within EX28DRAFT_1175 at 1197.655 kb on + strand, within EX28DRAFT_1175 at 1197.657 kb on + strand, within EX28DRAFT_1175 at 1197.711 kb on + strand, within EX28DRAFT_1175 at 1197.712 kb on - strand, within EX28DRAFT_1175 at 1197.807 kb on + strand, within EX28DRAFT_1175 at 1197.807 kb on + strand, within EX28DRAFT_1175 at 1197.808 kb on - strand, within EX28DRAFT_1175 at 1197.951 kb on + strand at 1197.951 kb on + strand at 1197.951 kb on + strand at 1197.952 kb on - strand at 1197.952 kb on - strand at 1197.954 kb on - strand at 1198.217 kb on + strand, within EX28DRAFT_1176 at 1198.274 kb on + strand, within EX28DRAFT_1176 at 1198.275 kb on - strand, within EX28DRAFT_1176 at 1198.286 kb on + strand, within EX28DRAFT_1176 at 1198.286 kb on + strand, within EX28DRAFT_1176 at 1198.286 kb on + strand, within EX28DRAFT_1176 at 1198.287 kb on - strand, within EX28DRAFT_1176 at 1198.287 kb on - strand, within EX28DRAFT_1176 at 1198.289 kb on + strand, within EX28DRAFT_1176 at 1198.290 kb on - strand, within EX28DRAFT_1176 at 1198.359 kb on - strand at 1198.385 kb on + strand at 1198.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 1,196,227 + EX28DRAFT_1173 0.29 -0.9 1,196,263 - EX28DRAFT_1173 0.32 -1.1 1,196,285 + EX28DRAFT_1173 0.35 +0.1 1,196,286 - EX28DRAFT_1173 0.35 +1.3 1,196,287 + EX28DRAFT_1173 0.35 -1.1 1,196,288 - EX28DRAFT_1173 0.35 -0.6 1,196,291 + EX28DRAFT_1173 0.35 -0.0 1,196,292 - EX28DRAFT_1173 0.35 +0.6 1,196,317 - EX28DRAFT_1173 0.38 -1.9 1,196,362 - EX28DRAFT_1173 0.43 +0.5 1,196,394 - EX28DRAFT_1173 0.46 -0.2 1,196,394 - EX28DRAFT_1173 0.46 +0.5 1,196,484 + EX28DRAFT_1173 0.55 +0.9 1,196,676 + EX28DRAFT_1173 0.75 -0.7 1,196,705 - EX28DRAFT_1173 0.78 +0.0 1,196,811 + EX28DRAFT_1173 0.89 -0.5 1,196,845 - -0.2 1,196,888 - +0.4 1,196,888 - -1.2 1,196,912 + -0.7 1,196,913 - -0.4 1,196,918 - +0.1 1,196,948 - -2.2 1,196,954 + +0.2 1,196,957 - +0.4 1,197,361 + EX28DRAFT_1174 0.84 -0.0 1,197,361 + EX28DRAFT_1174 0.84 -0.3 1,197,384 - EX28DRAFT_1174 0.89 -0.6 1,197,429 + -0.5 1,197,431 + -0.8 1,197,431 + +1.3 1,197,431 + +0.7 1,197,431 + +0.0 1,197,431 + +0.6 1,197,432 - +0.2 1,197,432 - +0.2 1,197,432 - +0.0 1,197,435 + -0.0 1,197,435 + +0.4 1,197,436 - -0.7 1,197,436 - +0.1 1,197,436 - -0.3 1,197,436 - +1.4 1,197,436 - -0.8 1,197,437 + +0.8 1,197,438 - +1.0 1,197,438 - +0.3 1,197,438 - -0.6 1,197,438 - +0.8 1,197,438 - +0.4 1,197,438 - -0.6 1,197,440 + -0.1 1,197,440 + -0.1 1,197,643 + EX28DRAFT_1175 0.40 -0.4 1,197,646 - EX28DRAFT_1175 0.41 -2.0 1,197,655 + EX28DRAFT_1175 0.43 -0.1 1,197,657 + EX28DRAFT_1175 0.43 +0.6 1,197,711 + EX28DRAFT_1175 0.56 +0.5 1,197,712 - EX28DRAFT_1175 0.56 -0.1 1,197,807 + EX28DRAFT_1175 0.78 -0.0 1,197,807 + EX28DRAFT_1175 0.78 -0.1 1,197,808 - EX28DRAFT_1175 0.78 -2.4 1,197,951 + +0.6 1,197,951 + +0.0 1,197,951 + +1.2 1,197,952 - -2.3 1,197,952 - +0.6 1,197,954 - -0.2 1,198,217 + EX28DRAFT_1176 0.60 -1.4 1,198,274 + EX28DRAFT_1176 0.76 -0.9 1,198,275 - EX28DRAFT_1176 0.76 +0.6 1,198,286 + EX28DRAFT_1176 0.79 +0.3 1,198,286 + EX28DRAFT_1176 0.79 +0.8 1,198,286 + EX28DRAFT_1176 0.79 +2.0 1,198,287 - EX28DRAFT_1176 0.79 +0.8 1,198,287 - EX28DRAFT_1176 0.79 -0.3 1,198,289 + EX28DRAFT_1176 0.80 +0.3 1,198,290 - EX28DRAFT_1176 0.80 -0.1 1,198,359 - +0.1 1,198,385 + -0.6 1,198,429 + -0.2
Or see this region's nucleotide sequence