Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0927 and EX28DRAFT_0928 are separated by 46 nucleotides EX28DRAFT_0928 and EX28DRAFT_0929 are separated by 122 nucleotides EX28DRAFT_0929 and EX28DRAFT_0930 overlap by 4 nucleotides EX28DRAFT_0930 and EX28DRAFT_0931 are separated by 620 nucleotides
EX28DRAFT_0927: EX28DRAFT_0927 - Outer membrane protein W, at 926,708 to 927,340
_0927
EX28DRAFT_0928: EX28DRAFT_0928 - hypothetical protein, at 927,387 to 927,728
_0928
EX28DRAFT_0929: EX28DRAFT_0929 - Predicted redox protein, regulator of disulfide bond formation, at 927,851 to 928,084
_0929
EX28DRAFT_0930: EX28DRAFT_0930 - Predicted transporter component, at 928,081 to 929,292
_0930
EX28DRAFT_0931: EX28DRAFT_0931 - Response regulator, at 929,913 to 930,548
_0931
Position (kb)
928
929
930 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 927.231 kb on - strand, within EX28DRAFT_0927 at 927.252 kb on + strand, within EX28DRAFT_0927 at 927.252 kb on + strand, within EX28DRAFT_0927 at 927.289 kb on + strand at 927.290 kb on - strand at 927.309 kb on + strand at 927.309 kb on + strand at 927.313 kb on + strand at 927.313 kb on + strand at 927.314 kb on - strand at 927.314 kb on - strand at 927.454 kb on + strand, within EX28DRAFT_0928 at 927.455 kb on - strand, within EX28DRAFT_0928 at 927.455 kb on - strand, within EX28DRAFT_0928 at 927.455 kb on - strand, within EX28DRAFT_0928 at 927.456 kb on + strand, within EX28DRAFT_0928 at 927.456 kb on + strand, within EX28DRAFT_0928 at 927.456 kb on + strand, within EX28DRAFT_0928 at 927.456 kb on + strand, within EX28DRAFT_0928 at 927.456 kb on + strand, within EX28DRAFT_0928 at 927.457 kb on - strand, within EX28DRAFT_0928 at 927.457 kb on - strand, within EX28DRAFT_0928 at 927.461 kb on - strand, within EX28DRAFT_0928 at 927.461 kb on - strand, within EX28DRAFT_0928 at 927.461 kb on - strand, within EX28DRAFT_0928 at 927.595 kb on + strand, within EX28DRAFT_0928 at 927.638 kb on + strand, within EX28DRAFT_0928 at 927.639 kb on - strand, within EX28DRAFT_0928 at 927.721 kb on + strand at 927.722 kb on - strand at 927.770 kb on - strand at 928.111 kb on - strand at 928.182 kb on - strand at 928.182 kb on - strand at 928.352 kb on + strand, within EX28DRAFT_0930 at 928.425 kb on + strand, within EX28DRAFT_0930 at 928.425 kb on + strand, within EX28DRAFT_0930 at 928.470 kb on - strand, within EX28DRAFT_0930 at 928.479 kb on + strand, within EX28DRAFT_0930 at 928.513 kb on - strand, within EX28DRAFT_0930 at 928.566 kb on + strand, within EX28DRAFT_0930 at 928.814 kb on + strand, within EX28DRAFT_0930 at 928.815 kb on - strand, within EX28DRAFT_0930 at 929.115 kb on + strand, within EX28DRAFT_0930 at 929.134 kb on + strand, within EX28DRAFT_0930 at 929.134 kb on + strand, within EX28DRAFT_0930 at 929.201 kb on + strand at 929.202 kb on - strand at 929.202 kb on - strand at 929.202 kb on - strand at 929.202 kb on - strand at 929.204 kb on + strand at 929.205 kb on - strand at 929.208 kb on + strand at 929.265 kb on - strand at 929.297 kb on - strand at 929.297 kb on - strand at 929.343 kb on - strand at 929.344 kb on + strand at 929.345 kb on - strand at 929.485 kb on - strand at 929.486 kb on + strand at 929.486 kb on + strand at 929.590 kb on - strand at 929.603 kb on + strand at 929.649 kb on + strand at 929.650 kb on - strand at 929.680 kb on - strand at 929.709 kb on - strand at 929.709 kb on - strand at 929.811 kb on + strand at 929.827 kb on + strand at 929.878 kb on + strand at 929.883 kb on - strand at 929.955 kb on - strand at 929.962 kb on - strand at 930.061 kb on + strand, within EX28DRAFT_0931 at 930.062 kb on - strand, within EX28DRAFT_0931 at 930.062 kb on - strand, within EX28DRAFT_0931 at 930.064 kb on + strand, within EX28DRAFT_0931 at 930.107 kb on - strand, within EX28DRAFT_0931 at 930.181 kb on + strand, within EX28DRAFT_0931 at 930.242 kb on + strand, within EX28DRAFT_0931
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 927,231 - EX28DRAFT_0927 0.83 -0.8 927,252 + EX28DRAFT_0927 0.86 +0.2 927,252 + EX28DRAFT_0927 0.86 -0.0 927,289 + +0.4 927,290 - -0.2 927,309 + -0.8 927,309 + -0.5 927,313 + +0.2 927,313 + -0.1 927,314 - +1.4 927,314 - -0.5 927,454 + EX28DRAFT_0928 0.20 -1.5 927,455 - EX28DRAFT_0928 0.20 -1.0 927,455 - EX28DRAFT_0928 0.20 -0.5 927,455 - EX28DRAFT_0928 0.20 -0.0 927,456 + EX28DRAFT_0928 0.20 -0.7 927,456 + EX28DRAFT_0928 0.20 -1.1 927,456 + EX28DRAFT_0928 0.20 -1.1 927,456 + EX28DRAFT_0928 0.20 +0.4 927,456 + EX28DRAFT_0928 0.20 +0.1 927,457 - EX28DRAFT_0928 0.20 +1.7 927,457 - EX28DRAFT_0928 0.20 -1.0 927,461 - EX28DRAFT_0928 0.22 -2.0 927,461 - EX28DRAFT_0928 0.22 +0.5 927,461 - EX28DRAFT_0928 0.22 +0.0 927,595 + EX28DRAFT_0928 0.61 -0.3 927,638 + EX28DRAFT_0928 0.73 +0.4 927,639 - EX28DRAFT_0928 0.74 +0.0 927,721 + +1.5 927,722 - -0.7 927,770 - -0.0 928,111 - -0.4 928,182 - +2.0 928,182 - +0.6 928,352 + EX28DRAFT_0930 0.22 -0.1 928,425 + EX28DRAFT_0930 0.28 -1.3 928,425 + EX28DRAFT_0930 0.28 -0.3 928,470 - EX28DRAFT_0930 0.32 +0.2 928,479 + EX28DRAFT_0930 0.33 -0.5 928,513 - EX28DRAFT_0930 0.36 +0.0 928,566 + EX28DRAFT_0930 0.40 -0.5 928,814 + EX28DRAFT_0930 0.60 +1.7 928,815 - EX28DRAFT_0930 0.61 -0.9 929,115 + EX28DRAFT_0930 0.85 -0.4 929,134 + EX28DRAFT_0930 0.87 +0.0 929,134 + EX28DRAFT_0930 0.87 +0.4 929,201 + -0.1 929,202 - -2.8 929,202 - +0.2 929,202 - -0.3 929,202 - +2.3 929,204 + -0.2 929,205 - +0.1 929,208 + +1.4 929,265 - -0.3 929,297 - +0.1 929,297 - +0.2 929,343 - +0.3 929,344 + -0.5 929,345 - +0.8 929,485 - +0.2 929,486 + -0.6 929,486 + -0.7 929,590 - +1.0 929,603 + +0.6 929,649 + +0.1 929,650 - +0.6 929,680 - +0.2 929,709 - +0.0 929,709 - +0.2 929,811 + -0.3 929,827 + -0.3 929,878 + +0.1 929,883 - -0.7 929,955 - -0.4 929,962 - +0.1 930,061 + EX28DRAFT_0931 0.23 -0.8 930,062 - EX28DRAFT_0931 0.23 -0.0 930,062 - EX28DRAFT_0931 0.23 +0.2 930,064 + EX28DRAFT_0931 0.24 +1.8 930,107 - EX28DRAFT_0931 0.31 +0.3 930,181 + EX28DRAFT_0931 0.42 -0.2 930,242 + EX28DRAFT_0931 0.52 +1.3
Or see this region's nucleotide sequence