Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0736 and EX28DRAFT_0737 are separated by 65 nucleotides EX28DRAFT_0737 and EX28DRAFT_0738 overlap by 4 nucleotides EX28DRAFT_0738 and EX28DRAFT_0739 are separated by 398 nucleotides EX28DRAFT_0739 and EX28DRAFT_0740 are separated by 54 nucleotides
EX28DRAFT_0736: EX28DRAFT_0736 - Cell division protein FtsI/penicillin-binding protein 2, at 732,795 to 734,513
_0736
EX28DRAFT_0737: EX28DRAFT_0737 - Methyltransferase domain, at 734,579 to 735,394
_0737
EX28DRAFT_0738: EX28DRAFT_0738 - Predicted membrane protein, at 735,391 to 735,957
_0738
EX28DRAFT_0739: EX28DRAFT_0739 - Predicted membrane protein, at 736,356 to 736,814
_0739
EX28DRAFT_0740: EX28DRAFT_0740 - PTS system, mannose/fructose/sorbose family, IID component, at 736,869 to 737,720
_0740
Position (kb) 735
736 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 734.391 kb on + strand at 734.392 kb on - strand at 734.523 kb on + strand at 734.525 kb on + strand at 734.544 kb on + strand at 734.626 kb on + strand at 734.627 kb on - strand at 734.634 kb on - strand at 734.635 kb on + strand at 734.636 kb on - strand at 734.636 kb on - strand at 734.705 kb on - strand, within EX28DRAFT_0737 at 734.778 kb on - strand, within EX28DRAFT_0737 at 734.871 kb on - strand, within EX28DRAFT_0737 at 734.871 kb on - strand, within EX28DRAFT_0737 at 734.873 kb on + strand, within EX28DRAFT_0737 at 734.873 kb on + strand, within EX28DRAFT_0737 at 735.158 kb on + strand, within EX28DRAFT_0737 at 735.159 kb on - strand, within EX28DRAFT_0737 at 735.334 kb on - strand at 735.334 kb on - strand at 735.342 kb on + strand at 735.343 kb on - strand at 735.343 kb on - strand at 735.497 kb on - strand, within EX28DRAFT_0738 at 735.734 kb on - strand, within EX28DRAFT_0738 at 735.864 kb on + strand, within EX28DRAFT_0738 at 735.984 kb on + strand at 735.985 kb on - strand at 736.022 kb on + strand at 736.026 kb on + strand at 736.027 kb on - strand at 736.027 kb on - strand at 736.053 kb on + strand at 736.054 kb on - strand at 736.054 kb on - strand at 736.169 kb on - strand at 736.532 kb on + strand, within EX28DRAFT_0739 at 736.593 kb on + strand, within EX28DRAFT_0739 at 736.593 kb on + strand, within EX28DRAFT_0739 at 736.611 kb on + strand, within EX28DRAFT_0739 at 736.639 kb on + strand, within EX28DRAFT_0739 at 736.649 kb on + strand, within EX28DRAFT_0739 at 736.753 kb on + strand, within EX28DRAFT_0739 at 736.794 kb on + strand at 736.858 kb on - strand at 736.870 kb on + strand at 736.870 kb on + strand at 736.871 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 734,391 + -2.9 734,392 - -0.2 734,523 + -0.0 734,525 + -0.0 734,544 + -1.6 734,626 + +0.4 734,627 - -0.6 734,634 - -0.1 734,635 + -1.8 734,636 - -0.6 734,636 - -0.9 734,705 - EX28DRAFT_0737 0.15 -0.3 734,778 - EX28DRAFT_0737 0.24 -0.1 734,871 - EX28DRAFT_0737 0.36 +0.4 734,871 - EX28DRAFT_0737 0.36 -0.4 734,873 + EX28DRAFT_0737 0.36 -0.4 734,873 + EX28DRAFT_0737 0.36 +1.2 735,158 + EX28DRAFT_0737 0.71 +0.2 735,159 - EX28DRAFT_0737 0.71 -1.8 735,334 - -0.3 735,334 - -0.9 735,342 + -0.1 735,343 - -0.1 735,343 - -0.7 735,497 - EX28DRAFT_0738 0.19 -0.3 735,734 - EX28DRAFT_0738 0.60 -2.3 735,864 + EX28DRAFT_0738 0.83 -0.4 735,984 + +1.7 735,985 - -0.4 736,022 + -0.1 736,026 + -0.7 736,027 - -1.0 736,027 - -0.1 736,053 + -0.9 736,054 - -0.2 736,054 - -0.1 736,169 - -0.1 736,532 + EX28DRAFT_0739 0.38 +0.0 736,593 + EX28DRAFT_0739 0.52 -0.0 736,593 + EX28DRAFT_0739 0.52 -0.0 736,611 + EX28DRAFT_0739 0.56 -0.7 736,639 + EX28DRAFT_0739 0.62 +1.5 736,649 + EX28DRAFT_0739 0.64 +0.7 736,753 + EX28DRAFT_0739 0.86 -0.4 736,794 + +1.4 736,858 - -0.5 736,870 + -0.1 736,870 + +0.2 736,871 - +0.2
Or see this region's nucleotide sequence