Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0718 and EX28DRAFT_0719 are separated by 166 nucleotides EX28DRAFT_0719 and EX28DRAFT_0720 are separated by 34 nucleotides EX28DRAFT_0720 and EX28DRAFT_0721 are separated by 106 nucleotides
EX28DRAFT_0718: EX28DRAFT_0718 - Uncharacterized protein conserved in bacteria, at 715,202 to 716,143
_0718
EX28DRAFT_0719: EX28DRAFT_0719 - Calcineurin-like phosphoesterase, at 716,310 to 716,954
_0719
EX28DRAFT_0720: EX28DRAFT_0720 - Protein of unknown function (DUF1480), at 716,989 to 717,228
_0720
EX28DRAFT_0721: EX28DRAFT_0721 - NOL1/NOP2/sun family putative RNA methylase, at 717,335 to 718,777
_0721
Position (kb) 716
717 Strain fitness (log2 ratio) -1
0
1 at 715.377 kb on + strand, within EX28DRAFT_0718 at 715.737 kb on + strand, within EX28DRAFT_0718 at 715.743 kb on - strand, within EX28DRAFT_0718 at 715.921 kb on + strand, within EX28DRAFT_0718 at 716.013 kb on - strand, within EX28DRAFT_0718 at 716.092 kb on + strand at 716.093 kb on - strand at 716.093 kb on - strand at 716.093 kb on - strand at 716.137 kb on + strand at 716.138 kb on - strand at 716.164 kb on - strand at 716.172 kb on - strand at 716.176 kb on + strand at 716.188 kb on - strand at 716.198 kb on + strand at 716.201 kb on + strand at 716.290 kb on + strand at 716.290 kb on + strand at 716.291 kb on - strand at 716.309 kb on + strand at 716.309 kb on + strand at 716.310 kb on - strand at 716.314 kb on - strand at 716.314 kb on - strand at 716.365 kb on + strand at 716.414 kb on + strand, within EX28DRAFT_0719 at 716.431 kb on + strand, within EX28DRAFT_0719 at 716.432 kb on - strand, within EX28DRAFT_0719 at 716.477 kb on + strand, within EX28DRAFT_0719 at 716.491 kb on + strand, within EX28DRAFT_0719 at 716.672 kb on + strand, within EX28DRAFT_0719 at 716.804 kb on - strand, within EX28DRAFT_0719 at 716.858 kb on + strand, within EX28DRAFT_0719 at 716.858 kb on + strand, within EX28DRAFT_0719 at 716.886 kb on - strand, within EX28DRAFT_0719 at 716.886 kb on - strand, within EX28DRAFT_0719 at 716.898 kb on + strand at 716.899 kb on - strand at 716.906 kb on + strand at 716.928 kb on + strand at 716.928 kb on + strand at 716.953 kb on - strand at 716.955 kb on + strand at 716.955 kb on + strand at 716.985 kb on + strand at 716.985 kb on + strand at 717.022 kb on - strand, within EX28DRAFT_0720 at 717.029 kb on + strand, within EX28DRAFT_0720 at 717.029 kb on + strand, within EX28DRAFT_0720 at 717.029 kb on + strand, within EX28DRAFT_0720 at 717.067 kb on + strand, within EX28DRAFT_0720 at 717.208 kb on - strand at 717.686 kb on + strand, within EX28DRAFT_0721 at 717.695 kb on - strand, within EX28DRAFT_0721 at 717.781 kb on + strand, within EX28DRAFT_0721
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 715,377 + EX28DRAFT_0718 0.19 +0.1 715,737 + EX28DRAFT_0718 0.57 +0.8 715,743 - EX28DRAFT_0718 0.57 +0.7 715,921 + EX28DRAFT_0718 0.76 -0.4 716,013 - EX28DRAFT_0718 0.86 +0.1 716,092 + -0.7 716,093 - +1.0 716,093 - +0.3 716,093 - -0.8 716,137 + +1.5 716,138 - +0.8 716,164 - +0.3 716,172 - -0.2 716,176 + -0.2 716,188 - +0.1 716,198 + -1.1 716,201 + +0.5 716,290 + -0.1 716,290 + +0.1 716,291 - +0.6 716,309 + -0.0 716,309 + -0.3 716,310 - +0.0 716,314 - -0.1 716,314 - -1.7 716,365 + -0.4 716,414 + EX28DRAFT_0719 0.16 -0.6 716,431 + EX28DRAFT_0719 0.19 -1.3 716,432 - EX28DRAFT_0719 0.19 +0.1 716,477 + EX28DRAFT_0719 0.26 -0.3 716,491 + EX28DRAFT_0719 0.28 -0.0 716,672 + EX28DRAFT_0719 0.56 -0.5 716,804 - EX28DRAFT_0719 0.77 -0.4 716,858 + EX28DRAFT_0719 0.85 -1.1 716,858 + EX28DRAFT_0719 0.85 -0.3 716,886 - EX28DRAFT_0719 0.89 +0.1 716,886 - EX28DRAFT_0719 0.89 -0.5 716,898 + -0.2 716,899 - -0.2 716,906 + +0.2 716,928 + +0.5 716,928 + +0.0 716,953 - -0.0 716,955 + -0.0 716,955 + -0.1 716,985 + -0.0 716,985 + +0.3 717,022 - EX28DRAFT_0720 0.14 -0.1 717,029 + EX28DRAFT_0720 0.17 -0.8 717,029 + EX28DRAFT_0720 0.17 +0.1 717,029 + EX28DRAFT_0720 0.17 -0.0 717,067 + EX28DRAFT_0720 0.33 -0.3 717,208 - +0.1 717,686 + EX28DRAFT_0721 0.24 +1.3 717,695 - EX28DRAFT_0721 0.25 -0.2 717,781 + EX28DRAFT_0721 0.31 -1.1
Or see this region's nucleotide sequence