Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0705 and EX28DRAFT_0706 are separated by 39 nucleotides EX28DRAFT_0706 and EX28DRAFT_0707 are separated by 42 nucleotides EX28DRAFT_0707 and EX28DRAFT_0708 are separated by 170 nucleotides EX28DRAFT_0708 and EX28DRAFT_0709 are separated by 85 nucleotides
EX28DRAFT_0705: EX28DRAFT_0705 - 6-phosphogluconate dehydratase (EC 4.2.1.12), at 703,606 to 705,417
_0705
EX28DRAFT_0706: EX28DRAFT_0706 - Entner-Doudoroff aldolase, at 705,457 to 706,098
_0706
EX28DRAFT_0707: EX28DRAFT_0707 - formate-dependent phosphoribosylglycinamide formyltransferase (EC 6.3.4.-), at 706,141 to 707,319
_0707
EX28DRAFT_0708: EX28DRAFT_0708 - Uncharacterized protein conserved in bacteria, at 707,490 to 708,137
_0708
EX28DRAFT_0709: EX28DRAFT_0709 - Protease II, at 708,223 to 710,283
_0709
Position (kb)
706
707
708 Strain fitness (log2 ratio)
-1
0
1
2 at 705.194 kb on - strand, within EX28DRAFT_0705 at 705.263 kb on + strand at 705.264 kb on - strand at 705.310 kb on - strand at 705.405 kb on + strand at 705.406 kb on - strand at 705.408 kb on + strand at 705.415 kb on + strand at 705.431 kb on + strand at 705.431 kb on + strand at 705.545 kb on + strand, within EX28DRAFT_0706 at 705.923 kb on + strand, within EX28DRAFT_0706 at 705.962 kb on - strand, within EX28DRAFT_0706 at 706.097 kb on - strand at 706.270 kb on + strand, within EX28DRAFT_0707 at 706.270 kb on + strand, within EX28DRAFT_0707 at 706.409 kb on - strand, within EX28DRAFT_0707 at 706.818 kb on + strand, within EX28DRAFT_0707 at 706.819 kb on - strand, within EX28DRAFT_0707 at 707.106 kb on + strand, within EX28DRAFT_0707 at 707.150 kb on + strand, within EX28DRAFT_0707 at 707.151 kb on - strand, within EX28DRAFT_0707 at 707.151 kb on - strand, within EX28DRAFT_0707 at 707.219 kb on - strand at 707.320 kb on - strand at 707.475 kb on + strand at 707.643 kb on + strand, within EX28DRAFT_0708 at 707.649 kb on - strand, within EX28DRAFT_0708 at 707.706 kb on + strand, within EX28DRAFT_0708 at 707.831 kb on + strand, within EX28DRAFT_0708 at 707.832 kb on - strand, within EX28DRAFT_0708 at 707.920 kb on + strand, within EX28DRAFT_0708 at 707.921 kb on - strand, within EX28DRAFT_0708 at 707.989 kb on - strand, within EX28DRAFT_0708 at 708.045 kb on - strand, within EX28DRAFT_0708 at 708.192 kb on + strand at 708.193 kb on - strand at 708.193 kb on - strand at 708.194 kb on + strand at 708.194 kb on + strand at 708.194 kb on + strand at 708.194 kb on + strand at 708.194 kb on + strand at 708.194 kb on + strand at 708.195 kb on - strand at 708.195 kb on - strand at 708.202 kb on - strand at 708.243 kb on + strand at 708.289 kb on + strand at 708.289 kb on + strand at 708.289 kb on + strand at 708.289 kb on + strand at 708.290 kb on - strand at 708.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 705,194 - EX28DRAFT_0705 0.88 +0.4 705,263 + -0.0 705,264 - -1.0 705,310 - +0.7 705,405 + +0.1 705,406 - -0.0 705,408 + +0.0 705,415 + -0.2 705,431 + -1.6 705,431 + +0.2 705,545 + EX28DRAFT_0706 0.14 -1.2 705,923 + EX28DRAFT_0706 0.73 -1.3 705,962 - EX28DRAFT_0706 0.79 -0.6 706,097 - +0.1 706,270 + EX28DRAFT_0707 0.11 -0.8 706,270 + EX28DRAFT_0707 0.11 -0.2 706,409 - EX28DRAFT_0707 0.23 +0.1 706,818 + EX28DRAFT_0707 0.57 +1.1 706,819 - EX28DRAFT_0707 0.58 +0.5 707,106 + EX28DRAFT_0707 0.82 -1.3 707,150 + EX28DRAFT_0707 0.86 +1.3 707,151 - EX28DRAFT_0707 0.86 +0.2 707,151 - EX28DRAFT_0707 0.86 +1.3 707,219 - -0.6 707,320 - +0.3 707,475 + +1.1 707,643 + EX28DRAFT_0708 0.24 -1.3 707,649 - EX28DRAFT_0708 0.25 +0.4 707,706 + EX28DRAFT_0708 0.33 +0.7 707,831 + EX28DRAFT_0708 0.53 -0.4 707,832 - EX28DRAFT_0708 0.53 +0.4 707,920 + EX28DRAFT_0708 0.66 -0.2 707,921 - EX28DRAFT_0708 0.67 +0.6 707,989 - EX28DRAFT_0708 0.77 +1.1 708,045 - EX28DRAFT_0708 0.86 -0.6 708,192 + +1.0 708,193 - -0.0 708,193 - -0.2 708,194 + +2.6 708,194 + -0.2 708,194 + +0.1 708,194 + -0.6 708,194 + +0.5 708,194 + -0.3 708,195 - -0.1 708,195 - -0.8 708,202 - -0.8 708,243 + +0.1 708,289 + -0.2 708,289 + -0.2 708,289 + -0.2 708,289 + +1.2 708,290 - +0.3 708,290 - -1.2
Or see this region's nucleotide sequence