Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0701 and EX28DRAFT_0702 are separated by 45 nucleotides EX28DRAFT_0702 and EX28DRAFT_0703 are separated by 140 nucleotides
EX28DRAFT_0701: EX28DRAFT_0701 - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase, at 698,072 to 699,043
_0701
EX28DRAFT_0702: EX28DRAFT_0702 - pyruvate kinase, at 699,089 to 700,531
_0702
EX28DRAFT_0703: EX28DRAFT_0703 - transcriptional regulator, RpiR family, at 700,672 to 701,541
_0703
Position (kb) 699
700
701 Strain fitness (log2 ratio) -2
-1
0
1 at 699.075 kb on + strand at 699.183 kb on + strand at 699.228 kb on - strand at 699.228 kb on - strand at 699.341 kb on - strand, within EX28DRAFT_0702 at 699.482 kb on - strand, within EX28DRAFT_0702 at 699.916 kb on - strand, within EX28DRAFT_0702 at 699.916 kb on - strand, within EX28DRAFT_0702 at 699.957 kb on + strand, within EX28DRAFT_0702 at 700.054 kb on + strand, within EX28DRAFT_0702 at 700.190 kb on - strand, within EX28DRAFT_0702 at 700.190 kb on - strand, within EX28DRAFT_0702 at 700.190 kb on - strand, within EX28DRAFT_0702 at 700.191 kb on + strand, within EX28DRAFT_0702 at 700.386 kb on - strand, within EX28DRAFT_0702 at 700.423 kb on + strand at 700.423 kb on + strand at 700.533 kb on + strand at 700.533 kb on + strand at 700.534 kb on - strand at 700.534 kb on - strand at 700.534 kb on - strand at 700.534 kb on - strand at 700.534 kb on - strand at 700.534 kb on - strand at 700.537 kb on - strand at 700.635 kb on - strand at 700.642 kb on - strand at 700.642 kb on - strand at 700.652 kb on + strand at 700.653 kb on - strand at 700.653 kb on - strand at 700.671 kb on - strand at 700.691 kb on - strand at 700.691 kb on - strand at 700.691 kb on - strand at 700.691 kb on - strand at 700.774 kb on + strand, within EX28DRAFT_0703 at 700.775 kb on - strand, within EX28DRAFT_0703 at 700.840 kb on - strand, within EX28DRAFT_0703 at 700.842 kb on - strand, within EX28DRAFT_0703 at 700.842 kb on - strand, within EX28DRAFT_0703 at 700.842 kb on - strand, within EX28DRAFT_0703 at 700.906 kb on - strand, within EX28DRAFT_0703 at 700.966 kb on + strand, within EX28DRAFT_0703 at 700.966 kb on + strand, within EX28DRAFT_0703 at 700.967 kb on - strand, within EX28DRAFT_0703 at 701.009 kb on - strand, within EX28DRAFT_0703 at 701.032 kb on - strand, within EX28DRAFT_0703 at 701.066 kb on + strand, within EX28DRAFT_0703 at 701.170 kb on + strand, within EX28DRAFT_0703 at 701.170 kb on + strand, within EX28DRAFT_0703 at 701.262 kb on - strand, within EX28DRAFT_0703 at 701.262 kb on - strand, within EX28DRAFT_0703 at 701.262 kb on - strand, within EX28DRAFT_0703 at 701.262 kb on - strand, within EX28DRAFT_0703 at 701.292 kb on + strand, within EX28DRAFT_0703 at 701.298 kb on + strand, within EX28DRAFT_0703 at 701.303 kb on + strand, within EX28DRAFT_0703 at 701.303 kb on + strand, within EX28DRAFT_0703 at 701.303 kb on + strand, within EX28DRAFT_0703
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 699,075 + +0.1 699,183 + -0.2 699,228 - -0.3 699,228 - +0.9 699,341 - EX28DRAFT_0702 0.17 -1.3 699,482 - EX28DRAFT_0702 0.27 -0.3 699,916 - EX28DRAFT_0702 0.57 -1.6 699,916 - EX28DRAFT_0702 0.57 +0.2 699,957 + EX28DRAFT_0702 0.60 -0.9 700,054 + EX28DRAFT_0702 0.67 -0.4 700,190 - EX28DRAFT_0702 0.76 +0.9 700,190 - EX28DRAFT_0702 0.76 +0.4 700,190 - EX28DRAFT_0702 0.76 -2.1 700,191 + EX28DRAFT_0702 0.76 -0.9 700,386 - EX28DRAFT_0702 0.90 +0.7 700,423 + -0.4 700,423 + +0.4 700,533 + -0.5 700,533 + -0.5 700,534 - +0.2 700,534 - -0.9 700,534 - +0.4 700,534 - +0.3 700,534 - +0.2 700,534 - +0.7 700,537 - -0.4 700,635 - -0.2 700,642 - -0.2 700,642 - -0.2 700,652 + +0.3 700,653 - -0.3 700,653 - -0.0 700,671 - -0.0 700,691 - +0.1 700,691 - -0.0 700,691 - -0.5 700,691 - +0.4 700,774 + EX28DRAFT_0703 0.12 -0.1 700,775 - EX28DRAFT_0703 0.12 +0.6 700,840 - EX28DRAFT_0703 0.19 -0.3 700,842 - EX28DRAFT_0703 0.20 -0.2 700,842 - EX28DRAFT_0703 0.20 +0.2 700,842 - EX28DRAFT_0703 0.20 -0.3 700,906 - EX28DRAFT_0703 0.27 -0.0 700,966 + EX28DRAFT_0703 0.34 +0.2 700,966 + EX28DRAFT_0703 0.34 -0.1 700,967 - EX28DRAFT_0703 0.34 +0.5 701,009 - EX28DRAFT_0703 0.39 -0.1 701,032 - EX28DRAFT_0703 0.41 +0.3 701,066 + EX28DRAFT_0703 0.45 +0.5 701,170 + EX28DRAFT_0703 0.57 -0.3 701,170 + EX28DRAFT_0703 0.57 -0.2 701,262 - EX28DRAFT_0703 0.68 -0.7 701,262 - EX28DRAFT_0703 0.68 +1.7 701,262 - EX28DRAFT_0703 0.68 -0.3 701,262 - EX28DRAFT_0703 0.68 -1.4 701,292 + EX28DRAFT_0703 0.71 +0.5 701,298 + EX28DRAFT_0703 0.72 +0.2 701,303 + EX28DRAFT_0703 0.73 +0.0 701,303 + EX28DRAFT_0703 0.73 -0.2 701,303 + EX28DRAFT_0703 0.73 +0.3
Or see this region's nucleotide sequence