Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0538 and EX28DRAFT_0539 overlap by 4 nucleotides EX28DRAFT_0539 and EX28DRAFT_0540 are separated by 5 nucleotides EX28DRAFT_0540 and EX28DRAFT_0541 are separated by 45 nucleotides
EX28DRAFT_0538: EX28DRAFT_0538 - histidinol-phosphate aminotransferase, at 531,843 to 532,904
_0538
EX28DRAFT_0539: EX28DRAFT_0539 - histidinol dehydrogenase, at 532,901 to 534,205
_0539
EX28DRAFT_0540: EX28DRAFT_0540 - ATP phosphoribosyltransferase, at 534,211 to 535,110
_0540
EX28DRAFT_0541: EX28DRAFT_0541 - RNA, at 535,156 to 535,279
_0541
Position (kb) 532
533
534
535 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 531.906 kb on - strand at 531.939 kb on - strand at 531.956 kb on + strand, within EX28DRAFT_0538 at 532.018 kb on + strand, within EX28DRAFT_0538 at 532.019 kb on - strand, within EX28DRAFT_0538 at 532.022 kb on + strand, within EX28DRAFT_0538 at 532.023 kb on - strand, within EX28DRAFT_0538 at 532.082 kb on - strand, within EX28DRAFT_0538 at 532.355 kb on - strand, within EX28DRAFT_0538 at 532.576 kb on + strand, within EX28DRAFT_0538 at 532.589 kb on + strand, within EX28DRAFT_0538 at 532.590 kb on - strand, within EX28DRAFT_0538 at 532.590 kb on - strand, within EX28DRAFT_0538 at 532.600 kb on + strand, within EX28DRAFT_0538 at 532.705 kb on + strand, within EX28DRAFT_0538 at 532.856 kb on - strand at 533.179 kb on + strand, within EX28DRAFT_0539 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.661 kb on + strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.817 kb on + strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539 at 533.917 kb on + strand, within EX28DRAFT_0539 at 533.918 kb on - strand, within EX28DRAFT_0539 at 534.045 kb on + strand, within EX28DRAFT_0539 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.428 kb on + strand, within EX28DRAFT_0540 at 534.428 kb on + strand, within EX28DRAFT_0540 at 534.429 kb on - strand, within EX28DRAFT_0540 at 534.545 kb on + strand, within EX28DRAFT_0540 at 534.732 kb on + strand, within EX28DRAFT_0540 at 534.733 kb on - strand, within EX28DRAFT_0540 at 534.733 kb on - strand, within EX28DRAFT_0540 at 534.834 kb on - strand, within EX28DRAFT_0540 at 534.939 kb on + strand, within EX28DRAFT_0540 at 535.015 kb on - strand, within EX28DRAFT_0540 at 535.081 kb on - strand at 535.081 kb on - strand at 535.084 kb on + strand at 535.084 kb on + strand at 535.085 kb on - strand at 535.085 kb on - strand at 535.135 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 531,906 - -4.5 531,939 - -4.5 531,956 + EX28DRAFT_0538 0.11 -2.5 532,018 + EX28DRAFT_0538 0.16 -3.1 532,019 - EX28DRAFT_0538 0.17 -4.0 532,022 + EX28DRAFT_0538 0.17 -3.7 532,023 - EX28DRAFT_0538 0.17 -4.5 532,082 - EX28DRAFT_0538 0.23 -2.4 532,355 - EX28DRAFT_0538 0.48 -4.1 532,576 + EX28DRAFT_0538 0.69 -5.6 532,589 + EX28DRAFT_0538 0.70 -3.5 532,590 - EX28DRAFT_0538 0.70 -5.0 532,590 - EX28DRAFT_0538 0.70 -3.4 532,600 + EX28DRAFT_0538 0.71 -2.8 532,705 + EX28DRAFT_0538 0.81 -4.3 532,856 - -5.2 533,179 + EX28DRAFT_0539 0.21 -2.9 533,569 - EX28DRAFT_0539 0.51 -0.3 533,569 - EX28DRAFT_0539 0.51 -5.0 533,659 + EX28DRAFT_0539 0.58 -2.9 533,659 + EX28DRAFT_0539 0.58 -3.8 533,659 + EX28DRAFT_0539 0.58 -4.9 533,660 - EX28DRAFT_0539 0.58 -3.0 533,660 - EX28DRAFT_0539 0.58 -4.0 533,660 - EX28DRAFT_0539 0.58 -5.0 533,661 + EX28DRAFT_0539 0.58 -2.9 533,662 - EX28DRAFT_0539 0.58 -4.8 533,662 - EX28DRAFT_0539 0.58 -4.9 533,817 + EX28DRAFT_0539 0.70 -5.8 533,818 - EX28DRAFT_0539 0.70 -5.3 533,818 - EX28DRAFT_0539 0.70 -2.1 533,917 + EX28DRAFT_0539 0.78 -4.2 533,918 - EX28DRAFT_0539 0.78 -3.4 534,045 + EX28DRAFT_0539 0.88 -3.3 534,389 - EX28DRAFT_0540 0.20 -4.1 534,389 - EX28DRAFT_0540 0.20 -4.0 534,389 - EX28DRAFT_0540 0.20 -3.5 534,389 - EX28DRAFT_0540 0.20 -2.2 534,428 + EX28DRAFT_0540 0.24 -2.2 534,428 + EX28DRAFT_0540 0.24 -4.2 534,429 - EX28DRAFT_0540 0.24 -1.2 534,545 + EX28DRAFT_0540 0.37 -3.5 534,732 + EX28DRAFT_0540 0.58 -3.3 534,733 - EX28DRAFT_0540 0.58 -3.6 534,733 - EX28DRAFT_0540 0.58 -3.5 534,834 - EX28DRAFT_0540 0.69 -3.7 534,939 + EX28DRAFT_0540 0.81 -4.3 535,015 - EX28DRAFT_0540 0.89 -5.5 535,081 - -3.7 535,081 - -1.8 535,084 + -4.1 535,084 + -3.7 535,085 - -3.8 535,085 - -2.5 535,135 + -3.6
Or see this region's nucleotide sequence