Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0512 and EX28DRAFT_0513 are separated by 176 nucleotides EX28DRAFT_0513 and EX28DRAFT_0514 are separated by 234 nucleotides EX28DRAFT_0514 and EX28DRAFT_0515 are separated by 85 nucleotides
EX28DRAFT_0512: EX28DRAFT_0512 - colanic acid biosynthesis protein WcaM, at 505,666 to 507,057
_0512
EX28DRAFT_0513: EX28DRAFT_0513 - UDP-glucose pyrophosphorylase regulatory subunit, at 507,234 to 508,130
_0513
EX28DRAFT_0514: EX28DRAFT_0514 - RNA, at 508,365 to 508,403
_0514
EX28DRAFT_0515: EX28DRAFT_0515 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 508,489 to 509,571
_0515
Position (kb) 507
508
509 Strain fitness (log2 ratio) -1
0
1
2 at 506.371 kb on + strand, within EX28DRAFT_0512 at 506.372 kb on - strand, within EX28DRAFT_0512 at 506.450 kb on + strand, within EX28DRAFT_0512 at 506.478 kb on + strand, within EX28DRAFT_0512 at 506.505 kb on + strand, within EX28DRAFT_0512 at 506.507 kb on + strand, within EX28DRAFT_0512 at 506.537 kb on + strand, within EX28DRAFT_0512 at 506.561 kb on - strand, within EX28DRAFT_0512 at 506.586 kb on + strand, within EX28DRAFT_0512 at 506.637 kb on - strand, within EX28DRAFT_0512 at 506.637 kb on - strand, within EX28DRAFT_0512 at 506.647 kb on + strand, within EX28DRAFT_0512 at 506.682 kb on + strand, within EX28DRAFT_0512 at 506.710 kb on + strand, within EX28DRAFT_0512 at 506.955 kb on + strand at 507.034 kb on - strand at 507.056 kb on - strand at 507.066 kb on + strand at 507.067 kb on - strand at 507.105 kb on + strand at 507.151 kb on + strand at 507.152 kb on - strand at 507.152 kb on - strand at 507.239 kb on + strand at 507.281 kb on + strand at 507.281 kb on + strand at 507.281 kb on + strand at 507.282 kb on - strand at 507.308 kb on + strand at 507.348 kb on + strand, within EX28DRAFT_0513 at 507.506 kb on + strand, within EX28DRAFT_0513 at 507.507 kb on - strand, within EX28DRAFT_0513 at 507.507 kb on - strand, within EX28DRAFT_0513 at 507.507 kb on - strand, within EX28DRAFT_0513 at 507.572 kb on + strand, within EX28DRAFT_0513 at 507.573 kb on - strand, within EX28DRAFT_0513 at 507.642 kb on - strand, within EX28DRAFT_0513 at 507.667 kb on - strand, within EX28DRAFT_0513 at 507.754 kb on - strand, within EX28DRAFT_0513 at 507.770 kb on + strand, within EX28DRAFT_0513 at 507.868 kb on + strand, within EX28DRAFT_0513 at 507.868 kb on + strand, within EX28DRAFT_0513 at 507.869 kb on - strand, within EX28DRAFT_0513 at 507.939 kb on - strand, within EX28DRAFT_0513 at 507.993 kb on - strand, within EX28DRAFT_0513 at 507.993 kb on - strand, within EX28DRAFT_0513 at 508.015 kb on - strand, within EX28DRAFT_0513 at 508.038 kb on + strand, within EX28DRAFT_0513 at 508.039 kb on - strand, within EX28DRAFT_0513 at 508.040 kb on + strand, within EX28DRAFT_0513 at 508.040 kb on + strand, within EX28DRAFT_0513 at 508.041 kb on - strand at 508.041 kb on - strand at 508.041 kb on - strand at 508.060 kb on - strand at 508.181 kb on + strand at 508.249 kb on - strand at 508.350 kb on + strand at 508.351 kb on - strand at 508.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 506,371 + EX28DRAFT_0512 0.51 +1.5 506,372 - EX28DRAFT_0512 0.51 -0.1 506,450 + EX28DRAFT_0512 0.56 +0.2 506,478 + EX28DRAFT_0512 0.58 +0.3 506,505 + EX28DRAFT_0512 0.60 +0.4 506,507 + EX28DRAFT_0512 0.60 -0.5 506,537 + EX28DRAFT_0512 0.63 +0.3 506,561 - EX28DRAFT_0512 0.64 +0.4 506,586 + EX28DRAFT_0512 0.66 +1.4 506,637 - EX28DRAFT_0512 0.70 +0.1 506,637 - EX28DRAFT_0512 0.70 -0.4 506,647 + EX28DRAFT_0512 0.70 -0.3 506,682 + EX28DRAFT_0512 0.73 -0.3 506,710 + EX28DRAFT_0512 0.75 +0.5 506,955 + +0.1 507,034 - -0.1 507,056 - -0.8 507,066 + -1.4 507,067 - -0.5 507,105 + +0.2 507,151 + +1.2 507,152 - +1.1 507,152 - -0.2 507,239 + +0.1 507,281 + +0.4 507,281 + +0.2 507,281 + +0.6 507,282 - +1.1 507,308 + +0.4 507,348 + EX28DRAFT_0513 0.13 -0.0 507,506 + EX28DRAFT_0513 0.30 +1.1 507,507 - EX28DRAFT_0513 0.30 +0.8 507,507 - EX28DRAFT_0513 0.30 +0.0 507,507 - EX28DRAFT_0513 0.30 +0.1 507,572 + EX28DRAFT_0513 0.38 +0.6 507,573 - EX28DRAFT_0513 0.38 +0.8 507,642 - EX28DRAFT_0513 0.45 -0.2 507,667 - EX28DRAFT_0513 0.48 +0.7 507,754 - EX28DRAFT_0513 0.58 -0.2 507,770 + EX28DRAFT_0513 0.60 +0.1 507,868 + EX28DRAFT_0513 0.71 -0.9 507,868 + EX28DRAFT_0513 0.71 +0.3 507,869 - EX28DRAFT_0513 0.71 +0.7 507,939 - EX28DRAFT_0513 0.79 -0.4 507,993 - EX28DRAFT_0513 0.85 +0.6 507,993 - EX28DRAFT_0513 0.85 +2.0 508,015 - EX28DRAFT_0513 0.87 +0.6 508,038 + EX28DRAFT_0513 0.90 +0.5 508,039 - EX28DRAFT_0513 0.90 +0.7 508,040 + EX28DRAFT_0513 0.90 +1.8 508,040 + EX28DRAFT_0513 0.90 -0.5 508,041 - +0.1 508,041 - +2.1 508,041 - +1.0 508,060 - +0.8 508,181 + -1.2 508,249 - -0.2 508,350 + +0.1 508,351 - -0.0 508,351 - +1.3
Or see this region's nucleotide sequence