Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0508 and EX28DRAFT_0509 are separated by 1 nucleotides EX28DRAFT_0509 and EX28DRAFT_0510 are separated by 17 nucleotides
EX28DRAFT_0508: EX28DRAFT_0508 - Undecaprenyl-phosphate glucose phosphotransferase, at 500,264 to 501,658
_0508
EX28DRAFT_0509: EX28DRAFT_0509 - Membrane protein involved in the export of O-antigen and teichoic acid, at 501,660 to 503,138
_0509
EX28DRAFT_0510: EX28DRAFT_0510 - colanic acid biosynthesis pyruvyl transferase WcaK, at 503,156 to 504,436
_0510
Position (kb)
501
502
503
504 Strain fitness (log2 ratio)
-1
0
1
2
3 at 500.688 kb on - strand, within EX28DRAFT_0508 at 500.792 kb on + strand, within EX28DRAFT_0508 at 500.840 kb on + strand, within EX28DRAFT_0508 at 500.851 kb on + strand, within EX28DRAFT_0508 at 500.851 kb on + strand, within EX28DRAFT_0508 at 500.852 kb on - strand, within EX28DRAFT_0508 at 500.888 kb on + strand, within EX28DRAFT_0508 at 500.889 kb on - strand, within EX28DRAFT_0508 at 500.923 kb on + strand, within EX28DRAFT_0508 at 500.986 kb on + strand, within EX28DRAFT_0508 at 501.097 kb on + strand, within EX28DRAFT_0508 at 501.098 kb on - strand, within EX28DRAFT_0508 at 501.098 kb on - strand, within EX28DRAFT_0508 at 501.455 kb on + strand, within EX28DRAFT_0508 at 501.455 kb on + strand, within EX28DRAFT_0508 at 501.456 kb on - strand, within EX28DRAFT_0508 at 501.573 kb on - strand at 501.573 kb on - strand at 501.590 kb on - strand at 501.656 kb on + strand at 501.656 kb on + strand at 501.656 kb on + strand at 501.656 kb on + strand at 501.667 kb on + strand at 501.667 kb on + strand at 501.668 kb on - strand at 501.668 kb on - strand at 502.329 kb on + strand, within EX28DRAFT_0509 at 502.329 kb on + strand, within EX28DRAFT_0509 at 502.329 kb on + strand, within EX28DRAFT_0509 at 502.330 kb on - strand, within EX28DRAFT_0509 at 502.330 kb on - strand, within EX28DRAFT_0509 at 502.408 kb on - strand, within EX28DRAFT_0509 at 502.408 kb on - strand, within EX28DRAFT_0509 at 502.855 kb on - strand, within EX28DRAFT_0509 at 502.899 kb on + strand, within EX28DRAFT_0509 at 502.900 kb on - strand, within EX28DRAFT_0509 at 503.137 kb on - strand at 503.140 kb on + strand at 503.167 kb on - strand at 503.182 kb on + strand at 503.182 kb on + strand at 503.183 kb on - strand at 503.183 kb on - strand at 503.224 kb on - strand at 503.224 kb on - strand at 503.237 kb on - strand at 503.507 kb on + strand, within EX28DRAFT_0510 at 503.585 kb on - strand, within EX28DRAFT_0510 at 503.669 kb on + strand, within EX28DRAFT_0510 at 503.819 kb on + strand, within EX28DRAFT_0510 at 503.928 kb on - strand, within EX28DRAFT_0510 at 504.018 kb on - strand, within EX28DRAFT_0510 at 504.018 kb on - strand, within EX28DRAFT_0510 at 504.018 kb on - strand, within EX28DRAFT_0510 at 504.080 kb on + strand, within EX28DRAFT_0510
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 500,688 - EX28DRAFT_0508 0.30 +0.6 500,792 + EX28DRAFT_0508 0.38 +0.3 500,840 + EX28DRAFT_0508 0.41 -0.4 500,851 + EX28DRAFT_0508 0.42 +0.1 500,851 + EX28DRAFT_0508 0.42 -0.7 500,852 - EX28DRAFT_0508 0.42 +0.7 500,888 + EX28DRAFT_0508 0.45 +0.4 500,889 - EX28DRAFT_0508 0.45 +1.6 500,923 + EX28DRAFT_0508 0.47 -0.1 500,986 + EX28DRAFT_0508 0.52 +1.8 501,097 + EX28DRAFT_0508 0.60 -1.5 501,098 - EX28DRAFT_0508 0.60 +0.6 501,098 - EX28DRAFT_0508 0.60 +2.1 501,455 + EX28DRAFT_0508 0.85 +0.3 501,455 + EX28DRAFT_0508 0.85 +0.3 501,456 - EX28DRAFT_0508 0.85 +0.4 501,573 - +0.4 501,573 - +2.8 501,590 - +0.5 501,656 + +0.1 501,656 + +0.1 501,656 + +0.9 501,656 + +0.4 501,667 + +0.1 501,667 + +0.1 501,668 - -0.9 501,668 - -1.2 502,329 + EX28DRAFT_0509 0.45 +0.1 502,329 + EX28DRAFT_0509 0.45 -0.2 502,329 + EX28DRAFT_0509 0.45 -1.3 502,330 - EX28DRAFT_0509 0.45 +0.3 502,330 - EX28DRAFT_0509 0.45 +0.7 502,408 - EX28DRAFT_0509 0.51 -0.0 502,408 - EX28DRAFT_0509 0.51 +0.2 502,855 - EX28DRAFT_0509 0.81 +0.1 502,899 + EX28DRAFT_0509 0.84 -1.1 502,900 - EX28DRAFT_0509 0.84 -1.0 503,137 - +0.9 503,140 + +0.0 503,167 - -1.0 503,182 + +0.5 503,182 + +0.3 503,183 - +0.7 503,183 - -0.5 503,224 - +0.1 503,224 - +0.5 503,237 - -0.4 503,507 + EX28DRAFT_0510 0.27 +0.2 503,585 - EX28DRAFT_0510 0.33 +1.0 503,669 + EX28DRAFT_0510 0.40 -0.0 503,819 + EX28DRAFT_0510 0.52 -0.1 503,928 - EX28DRAFT_0510 0.60 +0.8 504,018 - EX28DRAFT_0510 0.67 +0.3 504,018 - EX28DRAFT_0510 0.67 +0.2 504,018 - EX28DRAFT_0510 0.67 +0.1 504,080 + EX28DRAFT_0510 0.72 +0.2
Or see this region's nucleotide sequence