Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0496 and EX28DRAFT_0497 overlap by 1 nucleotides EX28DRAFT_0497 and EX28DRAFT_0498 overlap by 4 nucleotides EX28DRAFT_0498 and EX28DRAFT_0499 overlap by 26 nucleotides
EX28DRAFT_0496: EX28DRAFT_0496 - colanic acid biosynthesis glycosyl transferase WcaA, at 488,351 to 489,214
_0496
EX28DRAFT_0497: EX28DRAFT_0497 - colanic acid biosynthesis acetyltransferase WcaB, at 489,214 to 489,705
_0497
EX28DRAFT_0498: EX28DRAFT_0498 - colanic acid biosynthesis glycosyl transferase WcaC, at 489,702 to 490,919
_0498
EX28DRAFT_0499: EX28DRAFT_0499 - putative colanic acid polymerase WcaD, at 490,894 to 492,114
_0499
Position (kb) 489
490
491 Strain fitness (log2 ratio) -2
-1
0
1 at 488.732 kb on + strand, within EX28DRAFT_0496 at 488.744 kb on + strand, within EX28DRAFT_0496 at 488.744 kb on + strand, within EX28DRAFT_0496 at 488.745 kb on - strand, within EX28DRAFT_0496 at 488.745 kb on - strand, within EX28DRAFT_0496 at 488.745 kb on - strand, within EX28DRAFT_0496 at 488.808 kb on - strand, within EX28DRAFT_0496 at 488.825 kb on + strand, within EX28DRAFT_0496 at 488.825 kb on + strand, within EX28DRAFT_0496 at 488.919 kb on - strand, within EX28DRAFT_0496 at 488.949 kb on - strand, within EX28DRAFT_0496 at 489.094 kb on + strand, within EX28DRAFT_0496 at 489.130 kb on + strand at 489.130 kb on + strand at 489.212 kb on + strand at 489.212 kb on + strand at 489.213 kb on - strand at 489.330 kb on + strand, within EX28DRAFT_0497 at 489.606 kb on + strand, within EX28DRAFT_0497 at 489.609 kb on + strand, within EX28DRAFT_0497 at 489.699 kb on + strand at 489.699 kb on + strand at 489.700 kb on - strand at 489.700 kb on - strand at 489.720 kb on - strand at 489.720 kb on - strand at 489.725 kb on - strand at 489.806 kb on + strand at 490.017 kb on + strand, within EX28DRAFT_0498 at 490.017 kb on + strand, within EX28DRAFT_0498 at 490.018 kb on - strand, within EX28DRAFT_0498 at 490.018 kb on - strand, within EX28DRAFT_0498 at 490.136 kb on + strand, within EX28DRAFT_0498 at 490.163 kb on + strand, within EX28DRAFT_0498 at 490.163 kb on + strand, within EX28DRAFT_0498 at 490.284 kb on - strand, within EX28DRAFT_0498 at 490.284 kb on - strand, within EX28DRAFT_0498 at 490.349 kb on + strand, within EX28DRAFT_0498 at 490.369 kb on + strand, within EX28DRAFT_0498 at 490.652 kb on + strand, within EX28DRAFT_0498 at 490.893 kb on + strand at 490.893 kb on + strand at 490.894 kb on - strand at 490.921 kb on + strand at 490.922 kb on - strand at 490.942 kb on + strand at 490.943 kb on - strand at 490.943 kb on - strand at 491.029 kb on + strand, within EX28DRAFT_0499 at 491.029 kb on + strand, within EX28DRAFT_0499 at 491.101 kb on + strand, within EX28DRAFT_0499 at 491.102 kb on - strand, within EX28DRAFT_0499 at 491.272 kb on - strand, within EX28DRAFT_0499 at 491.790 kb on - strand, within EX28DRAFT_0499 at 491.790 kb on - strand, within EX28DRAFT_0499 at 491.815 kb on + strand, within EX28DRAFT_0499
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 488,732 + EX28DRAFT_0496 0.44 +0.1 488,744 + EX28DRAFT_0496 0.45 +0.7 488,744 + EX28DRAFT_0496 0.45 -0.2 488,745 - EX28DRAFT_0496 0.46 +0.4 488,745 - EX28DRAFT_0496 0.46 -0.1 488,745 - EX28DRAFT_0496 0.46 +0.9 488,808 - EX28DRAFT_0496 0.53 -0.3 488,825 + EX28DRAFT_0496 0.55 +0.1 488,825 + EX28DRAFT_0496 0.55 +0.4 488,919 - EX28DRAFT_0496 0.66 -1.6 488,949 - EX28DRAFT_0496 0.69 -0.1 489,094 + EX28DRAFT_0496 0.86 +0.3 489,130 + +0.3 489,130 + -0.6 489,212 + -0.9 489,212 + -0.6 489,213 - +0.3 489,330 + EX28DRAFT_0497 0.24 -0.8 489,606 + EX28DRAFT_0497 0.80 +0.0 489,609 + EX28DRAFT_0497 0.80 +0.2 489,699 + +0.1 489,699 + -0.2 489,700 - -0.3 489,700 - -0.2 489,720 - -0.9 489,720 - -0.8 489,725 - +0.9 489,806 + +0.2 490,017 + EX28DRAFT_0498 0.26 -0.7 490,017 + EX28DRAFT_0498 0.26 +1.1 490,018 - EX28DRAFT_0498 0.26 -0.9 490,018 - EX28DRAFT_0498 0.26 -0.2 490,136 + EX28DRAFT_0498 0.36 -0.2 490,163 + EX28DRAFT_0498 0.38 -0.5 490,163 + EX28DRAFT_0498 0.38 +0.0 490,284 - EX28DRAFT_0498 0.48 -0.8 490,284 - EX28DRAFT_0498 0.48 +0.4 490,349 + EX28DRAFT_0498 0.53 -0.2 490,369 + EX28DRAFT_0498 0.55 +0.4 490,652 + EX28DRAFT_0498 0.78 +0.4 490,893 + -0.1 490,893 + -0.1 490,894 - +0.9 490,921 + -0.4 490,922 - -1.8 490,942 + +0.6 490,943 - -0.6 490,943 - +0.3 491,029 + EX28DRAFT_0499 0.11 -1.4 491,029 + EX28DRAFT_0499 0.11 +0.6 491,101 + EX28DRAFT_0499 0.17 +0.0 491,102 - EX28DRAFT_0499 0.17 +0.0 491,272 - EX28DRAFT_0499 0.31 -0.7 491,790 - EX28DRAFT_0499 0.73 -1.1 491,790 - EX28DRAFT_0499 0.73 +0.2 491,815 + EX28DRAFT_0499 0.75 +1.4
Or see this region's nucleotide sequence