Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0475 and EX28DRAFT_0476 are separated by 141 nucleotides EX28DRAFT_0476 and EX28DRAFT_0477 overlap by 6 nucleotides EX28DRAFT_0477 and EX28DRAFT_0478 are separated by 82 nucleotides EX28DRAFT_0478 and EX28DRAFT_0479 are separated by 168 nucleotides EX28DRAFT_0479 and EX28DRAFT_0480 overlap by 4 nucleotides
EX28DRAFT_0475: EX28DRAFT_0475 - lipid kinase YegS, at 458,572 to 459,471
_0475
EX28DRAFT_0476: EX28DRAFT_0476 - Uncharacterized conserved protein, at 459,613 to 460,113
_0476
EX28DRAFT_0477: EX28DRAFT_0477 - RNA, at 460,108 to 460,192
_0477
EX28DRAFT_0478: EX28DRAFT_0478 - Collagenase and related proteases, at 460,275 to 461,636
_0478
EX28DRAFT_0479: EX28DRAFT_0479 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 461,805 to 462,527
_0479
EX28DRAFT_0480: EX28DRAFT_0480 - Signal transduction histidine kinase, at 462,524 to 463,927
_0480
Position (kb) 460
461
462 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 459.324 kb on + strand, within EX28DRAFT_0475 at 459.327 kb on - strand, within EX28DRAFT_0475 at 459.327 kb on - strand, within EX28DRAFT_0475 at 459.499 kb on + strand at 459.507 kb on + strand at 459.508 kb on - strand at 459.508 kb on - strand at 459.509 kb on + strand at 459.556 kb on + strand at 459.557 kb on - strand at 459.573 kb on + strand at 459.597 kb on + strand at 459.598 kb on - strand at 459.664 kb on + strand, within EX28DRAFT_0476 at 459.768 kb on - strand, within EX28DRAFT_0476 at 459.789 kb on + strand, within EX28DRAFT_0476 at 459.790 kb on - strand, within EX28DRAFT_0476 at 459.813 kb on - strand, within EX28DRAFT_0476 at 459.813 kb on - strand, within EX28DRAFT_0476 at 459.930 kb on - strand, within EX28DRAFT_0476 at 459.930 kb on - strand, within EX28DRAFT_0476 at 459.930 kb on - strand, within EX28DRAFT_0476 at 459.967 kb on - strand, within EX28DRAFT_0476 at 459.970 kb on + strand, within EX28DRAFT_0476 at 459.971 kb on - strand, within EX28DRAFT_0476 at 460.112 kb on - strand at 460.131 kb on + strand, within EX28DRAFT_0477 at 460.141 kb on - strand, within EX28DRAFT_0477 at 460.164 kb on + strand, within EX28DRAFT_0477 at 460.201 kb on + strand at 460.272 kb on + strand at 460.277 kb on - strand at 460.588 kb on + strand, within EX28DRAFT_0478 at 460.611 kb on - strand, within EX28DRAFT_0478 at 460.751 kb on - strand, within EX28DRAFT_0478 at 460.885 kb on + strand, within EX28DRAFT_0478 at 461.631 kb on - strand at 461.724 kb on + strand at 461.725 kb on - strand at 461.725 kb on - strand at 461.746 kb on - strand at 461.751 kb on + strand at 461.752 kb on - strand at 461.787 kb on - strand at 461.787 kb on - strand at 461.807 kb on - strand at 461.851 kb on + strand at 461.852 kb on - strand at 462.209 kb on - strand, within EX28DRAFT_0479 at 462.209 kb on - strand, within EX28DRAFT_0479 at 462.209 kb on - strand, within EX28DRAFT_0479 at 462.342 kb on + strand, within EX28DRAFT_0479 at 462.382 kb on + strand, within EX28DRAFT_0479 at 462.382 kb on + strand, within EX28DRAFT_0479 at 462.383 kb on - strand, within EX28DRAFT_0479 at 462.439 kb on - strand, within EX28DRAFT_0479 at 462.442 kb on + strand, within EX28DRAFT_0479 at 462.528 kb on - strand at 462.528 kb on - strand at 462.539 kb on + strand at 462.557 kb on + strand at 462.557 kb on + strand at 462.558 kb on - strand at 462.563 kb on + strand at 462.564 kb on - strand at 462.564 kb on - strand at 462.568 kb on + strand at 462.569 kb on - strand at 462.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 459,324 + EX28DRAFT_0475 0.84 +0.1 459,327 - EX28DRAFT_0475 0.84 +0.5 459,327 - EX28DRAFT_0475 0.84 -0.2 459,499 + +0.1 459,507 + +0.3 459,508 - +0.1 459,508 - -0.0 459,509 + +0.4 459,556 + -0.1 459,557 - -2.0 459,573 + -3.7 459,597 + -0.7 459,598 - -0.8 459,664 + EX28DRAFT_0476 0.10 -0.4 459,768 - EX28DRAFT_0476 0.31 +0.2 459,789 + EX28DRAFT_0476 0.35 +0.6 459,790 - EX28DRAFT_0476 0.35 +0.9 459,813 - EX28DRAFT_0476 0.40 +0.2 459,813 - EX28DRAFT_0476 0.40 -0.2 459,930 - EX28DRAFT_0476 0.63 +0.4 459,930 - EX28DRAFT_0476 0.63 -0.6 459,930 - EX28DRAFT_0476 0.63 -0.5 459,967 - EX28DRAFT_0476 0.71 +0.1 459,970 + EX28DRAFT_0476 0.71 -0.4 459,971 - EX28DRAFT_0476 0.71 +0.2 460,112 - +0.6 460,131 + EX28DRAFT_0477 0.27 +0.6 460,141 - EX28DRAFT_0477 0.39 +0.5 460,164 + EX28DRAFT_0477 0.66 +0.7 460,201 + +0.3 460,272 + -3.3 460,277 - +0.1 460,588 + EX28DRAFT_0478 0.23 -0.3 460,611 - EX28DRAFT_0478 0.25 -1.2 460,751 - EX28DRAFT_0478 0.35 -0.5 460,885 + EX28DRAFT_0478 0.45 -0.9 461,631 - +0.2 461,724 + +0.4 461,725 - +1.0 461,725 - -0.2 461,746 - -0.3 461,751 + -0.3 461,752 - +0.0 461,787 - -1.4 461,787 - +2.7 461,807 - +0.7 461,851 + +0.6 461,852 - -0.4 462,209 - EX28DRAFT_0479 0.56 -0.2 462,209 - EX28DRAFT_0479 0.56 -0.6 462,209 - EX28DRAFT_0479 0.56 -0.2 462,342 + EX28DRAFT_0479 0.74 +0.8 462,382 + EX28DRAFT_0479 0.80 +0.4 462,382 + EX28DRAFT_0479 0.80 -0.8 462,383 - EX28DRAFT_0479 0.80 +0.1 462,439 - EX28DRAFT_0479 0.88 +0.0 462,442 + EX28DRAFT_0479 0.88 -0.6 462,528 - +0.1 462,528 - +0.0 462,539 + +0.4 462,557 + +0.6 462,557 + +0.8 462,558 - +2.4 462,563 + -1.2 462,564 - +0.4 462,564 - +1.4 462,568 + -0.2 462,569 - -0.5 462,617 - -0.6
Or see this region's nucleotide sequence