Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0432 and EX28DRAFT_0433 overlap by 17 nucleotides EX28DRAFT_0433 and EX28DRAFT_0434 overlap by 26 nucleotides EX28DRAFT_0434 and EX28DRAFT_0435 are separated by 69 nucleotides
EX28DRAFT_0432: EX28DRAFT_0432 - ABC-type proline/glycine betaine transport systems, ATPase components, at 413,569 to 414,519
_0432
EX28DRAFT_0433: EX28DRAFT_0433 - ABC-type proline/glycine betaine transport systems, permease component, at 414,503 to 415,240
_0433
EX28DRAFT_0434: EX28DRAFT_0434 - hypothetical protein, at 415,215 to 415,328
_0434
EX28DRAFT_0435: EX28DRAFT_0435 - Predicted transcriptional regulators, at 415,398 to 416,138
_0435
Position (kb) 415
416 Strain fitness (log2 ratio) -2
-1
0
1
2 at 414.233 kb on + strand, within EX28DRAFT_0432 at 414.236 kb on + strand, within EX28DRAFT_0432 at 414.237 kb on - strand, within EX28DRAFT_0432 at 414.237 kb on - strand, within EX28DRAFT_0432 at 414.237 kb on - strand, within EX28DRAFT_0432 at 414.325 kb on + strand, within EX28DRAFT_0432 at 414.326 kb on - strand, within EX28DRAFT_0432 at 414.326 kb on - strand, within EX28DRAFT_0432 at 414.326 kb on - strand, within EX28DRAFT_0432 at 414.326 kb on - strand, within EX28DRAFT_0432 at 414.326 kb on - strand, within EX28DRAFT_0432 at 414.327 kb on + strand, within EX28DRAFT_0432 at 414.328 kb on - strand, within EX28DRAFT_0432 at 414.640 kb on + strand, within EX28DRAFT_0433 at 414.641 kb on - strand, within EX28DRAFT_0433 at 414.641 kb on - strand, within EX28DRAFT_0433 at 414.643 kb on + strand, within EX28DRAFT_0433 at 414.936 kb on + strand, within EX28DRAFT_0433 at 415.106 kb on - strand, within EX28DRAFT_0433 at 415.108 kb on - strand, within EX28DRAFT_0433 at 415.129 kb on + strand, within EX28DRAFT_0433 at 415.137 kb on - strand, within EX28DRAFT_0433 at 415.137 kb on - strand, within EX28DRAFT_0433 at 415.211 kb on + strand at 415.304 kb on + strand, within EX28DRAFT_0434 at 415.357 kb on + strand at 415.358 kb on - strand at 415.358 kb on - strand at 415.389 kb on + strand at 415.389 kb on + strand at 415.390 kb on - strand at 415.533 kb on - strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.694 kb on - strand, within EX28DRAFT_0435 at 415.694 kb on - strand, within EX28DRAFT_0435 at 415.694 kb on - strand, within EX28DRAFT_0435 at 416.049 kb on - strand, within EX28DRAFT_0435 at 416.049 kb on - strand, within EX28DRAFT_0435 at 416.121 kb on + strand at 416.121 kb on + strand at 416.122 kb on - strand at 416.128 kb on + strand at 416.170 kb on - strand at 416.175 kb on + strand at 416.175 kb on + strand at 416.175 kb on + strand at 416.306 kb on - strand at 416.322 kb on - strand at 416.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 414,233 + EX28DRAFT_0432 0.70 +1.1 414,236 + EX28DRAFT_0432 0.70 +0.3 414,237 - EX28DRAFT_0432 0.70 +0.3 414,237 - EX28DRAFT_0432 0.70 +0.4 414,237 - EX28DRAFT_0432 0.70 +0.3 414,325 + EX28DRAFT_0432 0.79 -0.6 414,326 - EX28DRAFT_0432 0.80 +0.2 414,326 - EX28DRAFT_0432 0.80 +0.9 414,326 - EX28DRAFT_0432 0.80 +0.2 414,326 - EX28DRAFT_0432 0.80 -2.2 414,326 - EX28DRAFT_0432 0.80 +0.6 414,327 + EX28DRAFT_0432 0.80 -0.2 414,328 - EX28DRAFT_0432 0.80 +0.1 414,640 + EX28DRAFT_0433 0.19 -0.6 414,641 - EX28DRAFT_0433 0.19 -1.9 414,641 - EX28DRAFT_0433 0.19 +0.1 414,643 + EX28DRAFT_0433 0.19 +1.8 414,936 + EX28DRAFT_0433 0.59 +0.0 415,106 - EX28DRAFT_0433 0.82 +0.5 415,108 - EX28DRAFT_0433 0.82 -0.1 415,129 + EX28DRAFT_0433 0.85 +0.7 415,137 - EX28DRAFT_0433 0.86 -0.4 415,137 - EX28DRAFT_0433 0.86 -0.6 415,211 + +0.1 415,304 + EX28DRAFT_0434 0.78 -0.8 415,357 + -1.0 415,358 - +0.3 415,358 - -0.4 415,389 + -0.3 415,389 + -0.1 415,390 - -0.3 415,533 - EX28DRAFT_0435 0.18 +0.3 415,693 + EX28DRAFT_0435 0.40 -0.3 415,693 + EX28DRAFT_0435 0.40 -0.7 415,693 + EX28DRAFT_0435 0.40 -0.3 415,693 + EX28DRAFT_0435 0.40 +0.3 415,694 - EX28DRAFT_0435 0.40 -0.3 415,694 - EX28DRAFT_0435 0.40 +0.1 415,694 - EX28DRAFT_0435 0.40 -0.2 416,049 - EX28DRAFT_0435 0.88 +0.7 416,049 - EX28DRAFT_0435 0.88 +0.5 416,121 + -0.1 416,121 + +0.4 416,122 - -0.5 416,128 + +0.5 416,170 - -0.1 416,175 + -0.3 416,175 + +1.6 416,175 + -0.2 416,306 - +0.7 416,322 - -0.5 416,322 - -0.3
Or see this region's nucleotide sequence