Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0348 and EX28DRAFT_0349 overlap by 11 nucleotides EX28DRAFT_0349 and EX28DRAFT_0350 are separated by 252 nucleotides
EX28DRAFT_0348: EX28DRAFT_0348 - glycerol-3-phosphate dehydrogenase, anaerobic, B subunit, at 310,798 to 312,015
_0348
EX28DRAFT_0349: EX28DRAFT_0349 - glycerol-3-phosphate dehydrogenase, anaerobic, A subunit, at 312,005 to 313,633
_0349
EX28DRAFT_0350: EX28DRAFT_0350 - glycerol-3-phosphate transporter, at 313,886 to 315,238
_0350
Position (kb) 312
313
314 Strain fitness (log2 ratio) -2
-1
0
1
2 at 311.067 kb on + strand, within EX28DRAFT_0348 at 311.139 kb on + strand, within EX28DRAFT_0348 at 311.140 kb on - strand, within EX28DRAFT_0348 at 311.140 kb on - strand, within EX28DRAFT_0348 at 311.161 kb on - strand, within EX28DRAFT_0348 at 311.444 kb on + strand, within EX28DRAFT_0348 at 311.444 kb on + strand, within EX28DRAFT_0348 at 311.628 kb on + strand, within EX28DRAFT_0348 at 311.701 kb on + strand, within EX28DRAFT_0348 at 311.808 kb on + strand, within EX28DRAFT_0348 at 311.809 kb on - strand, within EX28DRAFT_0348 at 311.809 kb on - strand, within EX28DRAFT_0348 at 311.831 kb on + strand, within EX28DRAFT_0348 at 312.052 kb on - strand at 312.172 kb on + strand, within EX28DRAFT_0349 at 312.173 kb on - strand, within EX28DRAFT_0349 at 312.561 kb on + strand, within EX28DRAFT_0349 at 312.593 kb on - strand, within EX28DRAFT_0349 at 312.702 kb on + strand, within EX28DRAFT_0349 at 312.702 kb on + strand, within EX28DRAFT_0349 at 312.836 kb on + strand, within EX28DRAFT_0349 at 312.836 kb on + strand, within EX28DRAFT_0349 at 312.836 kb on + strand, within EX28DRAFT_0349 at 312.837 kb on - strand, within EX28DRAFT_0349 at 312.837 kb on - strand, within EX28DRAFT_0349 at 313.052 kb on - strand, within EX28DRAFT_0349 at 313.053 kb on + strand, within EX28DRAFT_0349 at 313.053 kb on + strand, within EX28DRAFT_0349 at 313.053 kb on + strand, within EX28DRAFT_0349 at 313.301 kb on - strand, within EX28DRAFT_0349 at 313.313 kb on - strand, within EX28DRAFT_0349 at 313.313 kb on - strand, within EX28DRAFT_0349 at 313.351 kb on + strand, within EX28DRAFT_0349 at 313.354 kb on + strand, within EX28DRAFT_0349 at 313.354 kb on + strand, within EX28DRAFT_0349 at 313.421 kb on - strand, within EX28DRAFT_0349 at 313.538 kb on + strand at 313.679 kb on - strand at 313.817 kb on + strand at 313.885 kb on + strand at 313.886 kb on - strand at 313.894 kb on + strand at 313.952 kb on + strand at 314.007 kb on - strand at 314.009 kb on + strand at 314.010 kb on - strand at 314.010 kb on - strand at 314.016 kb on + strand at 314.046 kb on - strand, within EX28DRAFT_0350 at 314.242 kb on + strand, within EX28DRAFT_0350 at 314.243 kb on - strand, within EX28DRAFT_0350 at 314.252 kb on - strand, within EX28DRAFT_0350 at 314.252 kb on - strand, within EX28DRAFT_0350 at 314.252 kb on - strand, within EX28DRAFT_0350 at 314.456 kb on + strand, within EX28DRAFT_0350 at 314.456 kb on + strand, within EX28DRAFT_0350 at 314.457 kb on - strand, within EX28DRAFT_0350 at 314.457 kb on - strand, within EX28DRAFT_0350 at 314.457 kb on - strand, within EX28DRAFT_0350 at 314.457 kb on - strand, within EX28DRAFT_0350 at 314.457 kb on - strand, within EX28DRAFT_0350 at 314.458 kb on + strand, within EX28DRAFT_0350 at 314.459 kb on - strand, within EX28DRAFT_0350 at 314.459 kb on - strand, within EX28DRAFT_0350 at 314.576 kb on + strand, within EX28DRAFT_0350 at 314.628 kb on - strand, within EX28DRAFT_0350
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 311,067 + EX28DRAFT_0348 0.22 +0.2 311,139 + EX28DRAFT_0348 0.28 -0.9 311,140 - EX28DRAFT_0348 0.28 +0.4 311,140 - EX28DRAFT_0348 0.28 +0.3 311,161 - EX28DRAFT_0348 0.30 +0.5 311,444 + EX28DRAFT_0348 0.53 +0.1 311,444 + EX28DRAFT_0348 0.53 +0.6 311,628 + EX28DRAFT_0348 0.68 +1.2 311,701 + EX28DRAFT_0348 0.74 +0.4 311,808 + EX28DRAFT_0348 0.83 +0.3 311,809 - EX28DRAFT_0348 0.83 +0.1 311,809 - EX28DRAFT_0348 0.83 -0.7 311,831 + EX28DRAFT_0348 0.85 +0.3 312,052 - -0.6 312,172 + EX28DRAFT_0349 0.10 +0.2 312,173 - EX28DRAFT_0349 0.10 +0.2 312,561 + EX28DRAFT_0349 0.34 +0.5 312,593 - EX28DRAFT_0349 0.36 -0.0 312,702 + EX28DRAFT_0349 0.43 +0.4 312,702 + EX28DRAFT_0349 0.43 -0.5 312,836 + EX28DRAFT_0349 0.51 +0.8 312,836 + EX28DRAFT_0349 0.51 -0.1 312,836 + EX28DRAFT_0349 0.51 +0.1 312,837 - EX28DRAFT_0349 0.51 -0.6 312,837 - EX28DRAFT_0349 0.51 +0.6 313,052 - EX28DRAFT_0349 0.64 +0.4 313,053 + EX28DRAFT_0349 0.64 +0.7 313,053 + EX28DRAFT_0349 0.64 +0.2 313,053 + EX28DRAFT_0349 0.64 +0.0 313,301 - EX28DRAFT_0349 0.80 -0.4 313,313 - EX28DRAFT_0349 0.80 -0.4 313,313 - EX28DRAFT_0349 0.80 +0.4 313,351 + EX28DRAFT_0349 0.83 +2.1 313,354 + EX28DRAFT_0349 0.83 -0.5 313,354 + EX28DRAFT_0349 0.83 +0.5 313,421 - EX28DRAFT_0349 0.87 +1.0 313,538 + -0.2 313,679 - -0.3 313,817 + +0.2 313,885 + +0.3 313,886 - -0.8 313,894 + -0.4 313,952 + +0.3 314,007 - +2.7 314,009 + -0.3 314,010 - +0.4 314,010 - -0.2 314,016 + +1.9 314,046 - EX28DRAFT_0350 0.12 -0.4 314,242 + EX28DRAFT_0350 0.26 +0.1 314,243 - EX28DRAFT_0350 0.26 -0.1 314,252 - EX28DRAFT_0350 0.27 -1.1 314,252 - EX28DRAFT_0350 0.27 -0.4 314,252 - EX28DRAFT_0350 0.27 -2.7 314,456 + EX28DRAFT_0350 0.42 +0.5 314,456 + EX28DRAFT_0350 0.42 +0.9 314,457 - EX28DRAFT_0350 0.42 +0.4 314,457 - EX28DRAFT_0350 0.42 -0.8 314,457 - EX28DRAFT_0350 0.42 -0.1 314,457 - EX28DRAFT_0350 0.42 +0.3 314,457 - EX28DRAFT_0350 0.42 -0.2 314,458 + EX28DRAFT_0350 0.42 -0.1 314,459 - EX28DRAFT_0350 0.42 +1.0 314,459 - EX28DRAFT_0350 0.42 +0.1 314,576 + EX28DRAFT_0350 0.51 +0.5 314,628 - EX28DRAFT_0350 0.55 +0.3
Or see this region's nucleotide sequence