Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0336 and EX28DRAFT_0337 are separated by 71 nucleotides EX28DRAFT_0337 and EX28DRAFT_0338 are separated by 53 nucleotides EX28DRAFT_0338 and EX28DRAFT_0339 are separated by 86 nucleotides EX28DRAFT_0339 and EX28DRAFT_0340 are separated by 88 nucleotides
EX28DRAFT_0336: EX28DRAFT_0336 - ribonuclease Z, at 298,720 to 299,637
_0336
EX28DRAFT_0337: EX28DRAFT_0337 - Predicted acyltransferase, at 299,709 to 300,170
_0337
EX28DRAFT_0338: EX28DRAFT_0338 - Uncharacterized conserved protein, at 300,224 to 300,529
_0338
EX28DRAFT_0339: EX28DRAFT_0339 - isochorismate synthase (EC 5.4.4.2), at 300,616 to 301,911
_0339
EX28DRAFT_0340: EX28DRAFT_0340 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase, at 302,000 to 303,670
_0340
Position (kb)
300
301
302 Strain fitness (log2 ratio)
-2
-1
0
1 at 299.617 kb on - strand at 299.673 kb on + strand at 299.674 kb on - strand at 299.676 kb on + strand at 299.678 kb on + strand at 299.729 kb on - strand at 299.729 kb on - strand at 299.729 kb on - strand at 299.729 kb on - strand at 299.814 kb on + strand, within EX28DRAFT_0337 at 299.859 kb on - strand, within EX28DRAFT_0337 at 299.893 kb on - strand, within EX28DRAFT_0337 at 300.045 kb on + strand, within EX28DRAFT_0337 at 300.046 kb on - strand, within EX28DRAFT_0337 at 300.078 kb on + strand, within EX28DRAFT_0337 at 300.079 kb on - strand, within EX28DRAFT_0337 at 300.143 kb on + strand at 300.188 kb on + strand at 300.189 kb on - strand at 300.206 kb on - strand at 300.208 kb on + strand at 300.208 kb on + strand at 300.209 kb on - strand at 300.209 kb on - strand at 300.224 kb on - strand at 300.297 kb on + strand, within EX28DRAFT_0338 at 300.297 kb on + strand, within EX28DRAFT_0338 at 300.329 kb on + strand, within EX28DRAFT_0338 at 300.329 kb on + strand, within EX28DRAFT_0338 at 300.330 kb on - strand, within EX28DRAFT_0338 at 300.331 kb on + strand, within EX28DRAFT_0338 at 300.331 kb on + strand, within EX28DRAFT_0338 at 300.331 kb on + strand, within EX28DRAFT_0338 at 300.332 kb on - strand, within EX28DRAFT_0338 at 300.400 kb on + strand, within EX28DRAFT_0338 at 300.400 kb on + strand, within EX28DRAFT_0338 at 300.400 kb on + strand, within EX28DRAFT_0338 at 300.401 kb on - strand, within EX28DRAFT_0338 at 300.404 kb on + strand, within EX28DRAFT_0338 at 300.405 kb on - strand, within EX28DRAFT_0338 at 300.405 kb on - strand, within EX28DRAFT_0338 at 300.405 kb on - strand, within EX28DRAFT_0338 at 300.407 kb on + strand, within EX28DRAFT_0338 at 300.431 kb on + strand, within EX28DRAFT_0338 at 300.431 kb on + strand, within EX28DRAFT_0338 at 300.446 kb on + strand, within EX28DRAFT_0338 at 300.446 kb on + strand, within EX28DRAFT_0338 at 300.447 kb on - strand, within EX28DRAFT_0338 at 300.548 kb on - strand at 300.548 kb on - strand at 300.549 kb on + strand at 300.549 kb on + strand at 300.550 kb on - strand at 300.550 kb on - strand at 300.563 kb on + strand at 300.563 kb on + strand at 300.564 kb on - strand at 300.568 kb on - strand at 300.568 kb on - strand at 300.586 kb on - strand at 300.589 kb on - strand at 300.589 kb on - strand at 300.689 kb on + strand at 300.775 kb on + strand, within EX28DRAFT_0339 at 301.071 kb on + strand, within EX28DRAFT_0339 at 301.072 kb on - strand, within EX28DRAFT_0339 at 301.271 kb on + strand, within EX28DRAFT_0339 at 301.279 kb on + strand, within EX28DRAFT_0339 at 301.470 kb on + strand, within EX28DRAFT_0339 at 301.470 kb on + strand, within EX28DRAFT_0339 at 301.488 kb on + strand, within EX28DRAFT_0339 at 301.529 kb on + strand, within EX28DRAFT_0339 at 301.558 kb on - strand, within EX28DRAFT_0339 at 301.632 kb on - strand, within EX28DRAFT_0339 at 301.739 kb on + strand, within EX28DRAFT_0339 at 301.824 kb on + strand at 301.872 kb on + strand at 301.910 kb on - strand at 301.937 kb on + strand at 301.965 kb on + strand at 301.965 kb on + strand at 301.966 kb on - strand at 301.966 kb on - strand at 301.966 kb on - strand at 301.968 kb on - strand at 302.018 kb on - strand at 302.081 kb on - strand at 302.081 kb on - strand at 302.081 kb on - strand at 302.304 kb on + strand, within EX28DRAFT_0340 at 302.425 kb on + strand, within EX28DRAFT_0340 at 302.426 kb on - strand, within EX28DRAFT_0340 at 302.426 kb on - strand, within EX28DRAFT_0340 at 302.495 kb on - strand, within EX28DRAFT_0340 at 302.522 kb on + strand, within EX28DRAFT_0340
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 299,617 - -0.2 299,673 + +0.0 299,674 - -0.3 299,676 + -0.1 299,678 + +1.0 299,729 - -0.1 299,729 - -0.1 299,729 - -1.0 299,729 - -0.6 299,814 + EX28DRAFT_0337 0.23 -2.5 299,859 - EX28DRAFT_0337 0.32 -0.3 299,893 - EX28DRAFT_0337 0.40 +0.3 300,045 + EX28DRAFT_0337 0.73 -0.9 300,046 - EX28DRAFT_0337 0.73 +0.4 300,078 + EX28DRAFT_0337 0.80 +0.8 300,079 - EX28DRAFT_0337 0.80 -0.3 300,143 + +0.5 300,188 + +0.9 300,189 - -1.3 300,206 - +0.1 300,208 + +0.3 300,208 + +0.0 300,209 - -0.5 300,209 - -0.1 300,224 - -0.2 300,297 + EX28DRAFT_0338 0.24 -0.2 300,297 + EX28DRAFT_0338 0.24 +0.4 300,329 + EX28DRAFT_0338 0.34 -0.6 300,329 + EX28DRAFT_0338 0.34 -1.3 300,330 - EX28DRAFT_0338 0.35 -0.3 300,331 + EX28DRAFT_0338 0.35 -0.1 300,331 + EX28DRAFT_0338 0.35 +0.9 300,331 + EX28DRAFT_0338 0.35 -1.7 300,332 - EX28DRAFT_0338 0.35 -0.3 300,400 + EX28DRAFT_0338 0.58 +0.3 300,400 + EX28DRAFT_0338 0.58 -0.3 300,400 + EX28DRAFT_0338 0.58 +0.6 300,401 - EX28DRAFT_0338 0.58 -0.3 300,404 + EX28DRAFT_0338 0.59 +0.2 300,405 - EX28DRAFT_0338 0.59 -0.0 300,405 - EX28DRAFT_0338 0.59 +0.2 300,405 - EX28DRAFT_0338 0.59 +0.2 300,407 + EX28DRAFT_0338 0.60 +0.4 300,431 + EX28DRAFT_0338 0.68 +0.3 300,431 + EX28DRAFT_0338 0.68 -0.2 300,446 + EX28DRAFT_0338 0.73 +1.5 300,446 + EX28DRAFT_0338 0.73 +0.3 300,447 - EX28DRAFT_0338 0.73 +0.1 300,548 - +1.2 300,548 - -2.7 300,549 + +0.5 300,549 + -1.7 300,550 - +0.3 300,550 - +0.7 300,563 + +0.5 300,563 + +0.7 300,564 - +0.6 300,568 - +0.7 300,568 - +1.1 300,586 - +0.4 300,589 - +1.2 300,589 - +0.2 300,689 + -0.2 300,775 + EX28DRAFT_0339 0.12 -0.3 301,071 + EX28DRAFT_0339 0.35 +0.1 301,072 - EX28DRAFT_0339 0.35 +0.4 301,271 + EX28DRAFT_0339 0.51 +0.4 301,279 + EX28DRAFT_0339 0.51 -0.4 301,470 + EX28DRAFT_0339 0.66 +1.5 301,470 + EX28DRAFT_0339 0.66 -0.4 301,488 + EX28DRAFT_0339 0.67 +0.6 301,529 + EX28DRAFT_0339 0.70 +0.4 301,558 - EX28DRAFT_0339 0.73 +0.1 301,632 - EX28DRAFT_0339 0.78 +0.1 301,739 + EX28DRAFT_0339 0.87 +0.4 301,824 + -0.1 301,872 + -0.0 301,910 - -0.4 301,937 + +1.7 301,965 + +0.4 301,965 + -0.7 301,966 - +0.4 301,966 - +0.2 301,966 - +0.3 301,968 - -1.3 302,018 - +0.2 302,081 - -0.1 302,081 - +0.4 302,081 - +0.3 302,304 + EX28DRAFT_0340 0.18 +1.0 302,425 + EX28DRAFT_0340 0.25 -1.1 302,426 - EX28DRAFT_0340 0.25 -0.2 302,426 - EX28DRAFT_0340 0.25 +0.5 302,495 - EX28DRAFT_0340 0.30 +0.5 302,522 + EX28DRAFT_0340 0.31 -1.0
Or see this region's nucleotide sequence