Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0292 and EX28DRAFT_0293 are separated by 89 nucleotides EX28DRAFT_0293 and EX28DRAFT_0294 are separated by 291 nucleotides EX28DRAFT_0294 and EX28DRAFT_0295 are separated by 226 nucleotides
EX28DRAFT_0292: EX28DRAFT_0292 - amidophosphoribosyltransferase (EC 2.4.2.14), at 254,313 to 255,830
_0292
EX28DRAFT_0293: EX28DRAFT_0293 - polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases, at 255,920 to 256,489
_0293
EX28DRAFT_0294: EX28DRAFT_0294 - lysine-arginine-ornithine-binding periplasmic protein, at 256,781 to 257,563
_0294
EX28DRAFT_0295: EX28DRAFT_0295 - lysine-arginine-ornithine-binding periplasmic protein, at 257,790 to 258,572
_0295
Position (kb) 256
257
258 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 255.809 kb on - strand at 256.545 kb on - strand at 256.545 kb on - strand at 256.560 kb on + strand at 256.574 kb on - strand at 256.664 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.746 kb on + strand at 256.747 kb on - strand at 256.747 kb on - strand at 256.750 kb on + strand at 256.754 kb on - strand at 256.764 kb on + strand at 256.764 kb on + strand at 256.821 kb on + strand at 256.838 kb on - strand at 256.868 kb on - strand, within EX28DRAFT_0294 at 256.887 kb on - strand, within EX28DRAFT_0294 at 256.887 kb on - strand, within EX28DRAFT_0294 at 257.047 kb on + strand, within EX28DRAFT_0294 at 257.285 kb on + strand, within EX28DRAFT_0294 at 257.285 kb on + strand, within EX28DRAFT_0294 at 257.401 kb on - strand, within EX28DRAFT_0294 at 257.509 kb on + strand at 257.510 kb on - strand at 257.513 kb on + strand at 257.514 kb on - strand at 257.514 kb on - strand at 257.514 kb on - strand at 257.534 kb on + strand at 257.535 kb on - strand at 257.550 kb on + strand at 257.562 kb on - strand at 257.636 kb on + strand at 257.655 kb on + strand at 257.667 kb on + strand at 257.667 kb on + strand at 257.699 kb on - strand at 257.733 kb on + strand at 257.733 kb on + strand at 257.734 kb on - strand at 257.895 kb on + strand, within EX28DRAFT_0295 at 257.896 kb on - strand, within EX28DRAFT_0295 at 257.896 kb on - strand, within EX28DRAFT_0295 at 257.896 kb on - strand, within EX28DRAFT_0295 at 257.990 kb on + strand, within EX28DRAFT_0295 at 257.991 kb on - strand, within EX28DRAFT_0295 at 257.991 kb on - strand, within EX28DRAFT_0295 at 257.991 kb on - strand, within EX28DRAFT_0295 at 257.991 kb on - strand, within EX28DRAFT_0295 at 257.994 kb on + strand, within EX28DRAFT_0295 at 257.995 kb on - strand, within EX28DRAFT_0295 at 257.995 kb on - strand, within EX28DRAFT_0295 at 257.995 kb on - strand, within EX28DRAFT_0295 at 258.056 kb on + strand, within EX28DRAFT_0295 at 258.057 kb on - strand, within EX28DRAFT_0295 at 258.057 kb on - strand, within EX28DRAFT_0295 at 258.057 kb on - strand, within EX28DRAFT_0295 at 258.057 kb on - strand, within EX28DRAFT_0295 at 258.290 kb on + strand, within EX28DRAFT_0295 at 258.290 kb on + strand, within EX28DRAFT_0295 at 258.294 kb on + strand, within EX28DRAFT_0295 at 258.294 kb on + strand, within EX28DRAFT_0295 at 258.294 kb on + strand, within EX28DRAFT_0295 at 258.295 kb on - strand, within EX28DRAFT_0295 at 258.387 kb on + strand, within EX28DRAFT_0295 at 258.388 kb on - strand, within EX28DRAFT_0295 at 258.407 kb on + strand, within EX28DRAFT_0295 at 258.411 kb on - strand, within EX28DRAFT_0295 at 258.411 kb on - strand, within EX28DRAFT_0295 at 258.522 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 255,809 - +0.5 256,545 - -0.0 256,545 - -0.0 256,560 + -0.6 256,574 - +0.5 256,664 + +0.2 256,746 + +0.7 256,746 + -0.8 256,746 + -0.4 256,746 + +0.1 256,746 + +0.2 256,747 - -0.3 256,747 - -0.5 256,750 + -0.5 256,754 - -0.8 256,764 + -0.4 256,764 + +1.4 256,821 + -2.4 256,838 - +0.5 256,868 - EX28DRAFT_0294 0.11 +0.8 256,887 - EX28DRAFT_0294 0.14 +0.3 256,887 - EX28DRAFT_0294 0.14 +1.3 257,047 + EX28DRAFT_0294 0.34 -0.4 257,285 + EX28DRAFT_0294 0.64 +0.3 257,285 + EX28DRAFT_0294 0.64 +0.3 257,401 - EX28DRAFT_0294 0.79 +0.4 257,509 + +0.2 257,510 - +0.6 257,513 + +0.7 257,514 - -0.7 257,514 - -0.4 257,514 - +1.1 257,534 + -0.0 257,535 - +0.0 257,550 + -0.0 257,562 - -1.2 257,636 + +3.1 257,655 + +0.2 257,667 + +0.5 257,667 + +0.5 257,699 - -0.0 257,733 + -3.1 257,733 + -0.9 257,734 - +1.3 257,895 + EX28DRAFT_0295 0.13 -0.0 257,896 - EX28DRAFT_0295 0.14 +1.0 257,896 - EX28DRAFT_0295 0.14 -0.5 257,896 - EX28DRAFT_0295 0.14 -0.4 257,990 + EX28DRAFT_0295 0.26 -0.7 257,991 - EX28DRAFT_0295 0.26 -1.5 257,991 - EX28DRAFT_0295 0.26 +0.3 257,991 - EX28DRAFT_0295 0.26 -0.0 257,991 - EX28DRAFT_0295 0.26 +1.1 257,994 + EX28DRAFT_0295 0.26 -2.6 257,995 - EX28DRAFT_0295 0.26 -0.6 257,995 - EX28DRAFT_0295 0.26 -0.6 257,995 - EX28DRAFT_0295 0.26 +0.2 258,056 + EX28DRAFT_0295 0.34 +0.1 258,057 - EX28DRAFT_0295 0.34 -2.0 258,057 - EX28DRAFT_0295 0.34 -0.9 258,057 - EX28DRAFT_0295 0.34 -0.8 258,057 - EX28DRAFT_0295 0.34 -0.9 258,290 + EX28DRAFT_0295 0.64 -0.1 258,290 + EX28DRAFT_0295 0.64 +0.5 258,294 + EX28DRAFT_0295 0.64 -0.3 258,294 + EX28DRAFT_0295 0.64 -0.9 258,294 + EX28DRAFT_0295 0.64 -1.7 258,295 - EX28DRAFT_0295 0.64 -0.3 258,387 + EX28DRAFT_0295 0.76 +0.1 258,388 - EX28DRAFT_0295 0.76 +0.6 258,407 + EX28DRAFT_0295 0.79 -0.7 258,411 - EX28DRAFT_0295 0.79 -0.0 258,411 - EX28DRAFT_0295 0.79 -0.5 258,522 + -0.4
Or see this region's nucleotide sequence