Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS28355 and ABID97_RS28360 overlap by 4 nucleotides ABID97_RS28360 and ABID97_RS28365 are separated by 80 nucleotides ABID97_RS28365 and ABID97_RS28370 are separated by 15 nucleotides ABID97_RS28370 and ABID97_RS28375 overlap by 4 nucleotides
ABID97_RS28355: ABID97_RS28355 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 591,291 to 592,334
_RS28355
ABID97_RS28360: ABID97_RS28360 - isochorismatase family protein, at 592,331 to 592,972
_RS28360
ABID97_RS28365: ABID97_RS28365 - ABC transporter substrate-binding protein, at 593,053 to 594,231
_RS28365
ABID97_RS28370: ABID97_RS28370 - branched-chain amino acid ABC transporter permease, at 594,247 to 595,116
_RS28370
ABID97_RS28375: ABID97_RS28375 - branched-chain amino acid ABC transporter permease, at 595,113 to 596,123
_RS28375
Position (kb) 593
594
595 Strain fitness (log2 ratio) -2
-1
0
1 at 593.254 kb on + strand, within ABID97_RS28365 at 593.400 kb on - strand, within ABID97_RS28365 at 593.400 kb on - strand, within ABID97_RS28365 at 593.470 kb on + strand, within ABID97_RS28365 at 593.870 kb on - strand, within ABID97_RS28365 at 594.230 kb on - strand at 594.821 kb on - strand, within ABID97_RS28370 at 594.821 kb on - strand, within ABID97_RS28370 at 595.021 kb on + strand, within ABID97_RS28370 at 595.022 kb on - strand, within ABID97_RS28370
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 593,254 + ABID97_RS28365 0.17 +0.6 593,400 - ABID97_RS28365 0.29 -1.0 593,400 - ABID97_RS28365 0.29 +0.9 593,470 + ABID97_RS28365 0.35 -2.1 593,870 - ABID97_RS28365 0.69 +0.2 594,230 - +0.6 594,821 - ABID97_RS28370 0.66 -0.2 594,821 - ABID97_RS28370 0.66 +0.5 595,021 + ABID97_RS28370 0.89 -0.2 595,022 - ABID97_RS28370 0.89 +0.1
Or see this region's nucleotide sequence