Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS17945 and ABID97_RS17950 are separated by 6 nucleotides ABID97_RS17950 and ABID97_RS17955 overlap by 4 nucleotides ABID97_RS17955 and ABID97_RS17960 overlap by 4 nucleotides
ABID97_RS17945: ABID97_RS17945 - bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II, at 3,708,806 to 3,709,960
_RS17945
ABID97_RS17950: ABID97_RS17950 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,709,967 to 3,710,434
_RS17950
ABID97_RS17955: ABID97_RS17955 - transcription antitermination factor NusB, at 3,710,431 to 3,710,967
_RS17955
ABID97_RS17960: ABID97_RS17960 - pyridoxal phosphate-dependent aminotransferase, at 3,710,964 to 3,712,160
_RS17960
Position (kb)
3709
3710
3711 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3709.829 kb on + strand, within ABID97_RS17945 at 3709.922 kb on + strand at 3711.003 kb on + strand at 3711.003 kb on + strand at 3711.003 kb on + strand at 3711.003 kb on + strand at 3711.003 kb on + strand at 3711.004 kb on - strand at 3711.004 kb on - strand at 3711.004 kb on - strand at 3711.004 kb on - strand at 3711.004 kb on - strand at 3711.004 kb on - strand at 3711.162 kb on + strand, within ABID97_RS17960 at 3711.346 kb on - strand, within ABID97_RS17960 at 3711.346 kb on - strand, within ABID97_RS17960
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 3,709,829 + ABID97_RS17945 0.89 +0.2 3,709,922 + +0.0 3,711,003 + -0.4 3,711,003 + -1.0 3,711,003 + -0.9 3,711,003 + -0.8 3,711,003 + -1.2 3,711,004 - -1.6 3,711,004 - -1.9 3,711,004 - -0.2 3,711,004 - -1.5 3,711,004 - -1.8 3,711,004 - -1.4 3,711,162 + ABID97_RS17960 0.17 -1.2 3,711,346 - ABID97_RS17960 0.32 -0.5 3,711,346 - ABID97_RS17960 0.32 -2.8
Or see this region's nucleotide sequence