Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS11395 and ABID97_RS11400 are separated by 33 nucleotides ABID97_RS11400 and ABID97_RS11405 are separated by 56 nucleotides ABID97_RS11405 and ABID97_RS11410 overlap by 4 nucleotides ABID97_RS11410 and ABID97_RS11415 are separated by 17 nucleotides ABID97_RS11415 and ABID97_RS11420 are separated by 261 nucleotides
ABID97_RS11395: ABID97_RS11395 - alpha/beta fold hydrolase, at 2,353,798 to 2,354,610
_RS11395
ABID97_RS11400: ABID97_RS11400 - FAD-dependent oxidoreductase, at 2,354,644 to 2,355,420
_RS11400
ABID97_RS11405: ABID97_RS11405 - 2Fe-2S iron-sulfur cluster binding domain-containing protein, at 2,355,477 to 2,355,755
_RS11405
ABID97_RS11410: ABID97_RS11410 - ferredoxin, at 2,355,752 to 2,356,079
_RS11410
ABID97_RS11415: ABID97_RS11415 - 2Fe-2S iron-sulfur cluster-binding protein, at 2,356,097 to 2,356,402
_RS11415
ABID97_RS11420: ABID97_RS11420 - iron-containing redox enzyme family protein, at 2,356,664 to 2,357,396
_RS11420
Position (kb)
2355
2356 Strain fitness (log2 ratio)
-1
0
1
2 at 2354.519 kb on + strand, within ABID97_RS11395 at 2354.519 kb on + strand, within ABID97_RS11395 at 2354.960 kb on + strand, within ABID97_RS11400 at 2355.422 kb on + strand at 2355.425 kb on - strand at 2355.478 kb on + strand at 2355.978 kb on + strand, within ABID97_RS11410 at 2356.475 kb on + strand at 2356.476 kb on - strand at 2356.578 kb on - strand at 2356.645 kb on - strand at 2356.675 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 2,354,519 + ABID97_RS11395 0.89 -0.6 2,354,519 + ABID97_RS11395 0.89 -0.5 2,354,960 + ABID97_RS11400 0.41 +0.4 2,355,422 + -0.7 2,355,425 - +0.2 2,355,478 + +2.1 2,355,978 + ABID97_RS11410 0.69 +0.4 2,356,475 + +0.1 2,356,476 - +0.2 2,356,578 - -0.1 2,356,645 - -0.2 2,356,675 - -0.5
Or see this region's nucleotide sequence