Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS00745 and ABID97_RS00750 are separated by 73 nucleotides ABID97_RS00750 and ABID97_RS00755 are separated by 13 nucleotides ABID97_RS00755 and ABID97_RS00760 are separated by 7 nucleotides ABID97_RS00760 and ABID97_RS00765 are separated by 8 nucleotides
ABID97_RS00745: ABID97_RS00745 - 2-hydroxychromene-2-carboxylate isomerase, at 148,079 to 148,684
_RS00745
ABID97_RS00750: ABID97_RS00750 - SDR family oxidoreductase, at 148,758 to 149,504
_RS00750
ABID97_RS00755: ABID97_RS00755 - MarC family protein, at 149,518 to 150,159
_RS00755
ABID97_RS00760: ABID97_RS00760 - EVE domain-containing protein, at 150,167 to 150,628
_RS00760
ABID97_RS00765: ABID97_RS00765 - ABC transporter substrate-binding protein, at 150,637 to 151,800
_RS00765
Position (kb) 149
150
151 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 148.635 kb on + strand at 148.635 kb on + strand at 148.635 kb on + strand at 148.662 kb on + strand at 148.662 kb on + strand at 148.663 kb on - strand at 148.663 kb on - strand at 148.663 kb on - strand at 148.663 kb on - strand at 148.663 kb on - strand at 148.704 kb on + strand at 148.704 kb on + strand at 148.704 kb on + strand at 148.705 kb on - strand at 148.705 kb on - strand at 148.705 kb on - strand at 148.705 kb on - strand at 148.705 kb on - strand at 148.724 kb on - strand at 148.768 kb on + strand at 148.768 kb on + strand at 148.855 kb on - strand, within ABID97_RS00750 at 148.855 kb on - strand, within ABID97_RS00750 at 149.177 kb on + strand, within ABID97_RS00750 at 149.178 kb on - strand, within ABID97_RS00750 at 149.217 kb on + strand, within ABID97_RS00750 at 149.218 kb on - strand, within ABID97_RS00750 at 149.463 kb on + strand at 149.464 kb on - strand at 149.464 kb on - strand at 149.464 kb on - strand at 149.502 kb on + strand at 149.511 kb on + strand at 149.594 kb on - strand, within ABID97_RS00755 at 149.605 kb on + strand, within ABID97_RS00755 at 149.924 kb on - strand, within ABID97_RS00755 at 149.924 kb on - strand, within ABID97_RS00755 at 150.369 kb on + strand, within ABID97_RS00760 at 150.372 kb on + strand, within ABID97_RS00760 at 150.373 kb on - strand, within ABID97_RS00760 at 150.468 kb on + strand, within ABID97_RS00760 at 150.468 kb on + strand, within ABID97_RS00760 at 150.469 kb on - strand, within ABID97_RS00760 at 150.618 kb on + strand at 150.619 kb on - strand at 150.619 kb on - strand at 150.619 kb on - strand at 150.650 kb on + strand at 150.651 kb on - strand at 150.651 kb on - strand at 150.651 kb on - strand at 150.731 kb on + strand at 150.731 kb on + strand at 150.731 kb on + strand at 150.732 kb on - strand at 150.861 kb on - strand, within ABID97_RS00765 at 150.866 kb on + strand, within ABID97_RS00765 at 150.867 kb on - strand, within ABID97_RS00765 at 150.981 kb on - strand, within ABID97_RS00765 at 151.028 kb on + strand, within ABID97_RS00765 at 151.028 kb on + strand, within ABID97_RS00765 at 151.029 kb on - strand, within ABID97_RS00765
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 148,635 + -2.0 148,635 + -1.6 148,635 + -2.0 148,662 + -2.0 148,662 + -0.6 148,663 - +1.0 148,663 - +1.4 148,663 - +1.0 148,663 - -3.5 148,663 - -0.6 148,704 + -1.6 148,704 + -2.5 148,704 + -2.3 148,705 - -1.7 148,705 - +1.0 148,705 - +0.4 148,705 - -0.6 148,705 - +0.1 148,724 - -0.7 148,768 + -1.0 148,768 + +1.3 148,855 - ABID97_RS00750 0.13 -0.6 148,855 - ABID97_RS00750 0.13 +0.2 149,177 + ABID97_RS00750 0.56 -0.6 149,178 - ABID97_RS00750 0.56 +0.3 149,217 + ABID97_RS00750 0.61 -0.4 149,218 - ABID97_RS00750 0.62 -1.1 149,463 + -1.8 149,464 - -0.7 149,464 - -0.3 149,464 - -0.6 149,502 + +0.1 149,511 + -1.1 149,594 - ABID97_RS00755 0.12 +0.1 149,605 + ABID97_RS00755 0.14 -0.0 149,924 - ABID97_RS00755 0.63 +0.8 149,924 - ABID97_RS00755 0.63 +0.4 150,369 + ABID97_RS00760 0.44 -0.4 150,372 + ABID97_RS00760 0.44 -0.6 150,373 - ABID97_RS00760 0.45 -1.1 150,468 + ABID97_RS00760 0.65 -0.7 150,468 + ABID97_RS00760 0.65 -0.1 150,469 - ABID97_RS00760 0.65 -0.3 150,618 + -0.4 150,619 - +2.0 150,619 - +0.4 150,619 - -0.4 150,650 + +0.0 150,651 - -0.4 150,651 - -0.7 150,651 - +0.3 150,731 + +0.2 150,731 + -0.2 150,731 + -0.0 150,732 - +2.0 150,861 - ABID97_RS00765 0.19 +0.0 150,866 + ABID97_RS00765 0.20 +0.7 150,867 - ABID97_RS00765 0.20 -0.3 150,981 - ABID97_RS00765 0.30 -0.5 151,028 + ABID97_RS00765 0.34 -0.0 151,028 + ABID97_RS00765 0.34 -1.0 151,029 - ABID97_RS00765 0.34 +0.7
Or see this region's nucleotide sequence