Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uhpB and uhpA overlap by 1 nucleotides uhpA and ilvN are separated by 75 nucleotides ilvN and ilvB are separated by 3 nucleotides
b3668: uhpB - sensor histidine protein kinase phosphorylates UhpA (VIMSS), at 3,846,657 to 3,848,159
uhpB
b3669: uhpA - DNA-binding response regulator in two-component regulatory system wtih UhpB (NCBI), at 3,848,159 to 3,848,749
uhpA
b3670: ilvN - acetolactate synthase small subunit (NCBI), at 3,848,825 to 3,849,115
ilvN
b3671: ilvB - acetolactate synthase large subunit (NCBI), at 3,849,119 to 3,850,807
ilvB
Position (kb)
3848
3849
3850 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3847.825 kb on - strand, within uhpB at 3847.825 kb on - strand, within uhpB at 3847.828 kb on - strand, within uhpB at 3847.828 kb on - strand, within uhpB at 3847.880 kb on - strand, within uhpB at 3847.883 kb on - strand, within uhpB at 3847.883 kb on - strand, within uhpB at 3847.942 kb on + strand, within uhpB at 3848.011 kb on - strand at 3848.016 kb on - strand at 3848.029 kb on - strand at 3848.029 kb on - strand at 3848.043 kb on + strand at 3848.045 kb on + strand at 3848.047 kb on + strand at 3848.074 kb on - strand at 3848.074 kb on - strand at 3848.083 kb on - strand at 3848.094 kb on - strand at 3848.129 kb on + strand at 3848.129 kb on + strand at 3848.163 kb on - strand at 3848.165 kb on + strand at 3848.193 kb on + strand at 3848.193 kb on + strand at 3848.196 kb on - strand at 3848.237 kb on + strand, within uhpA at 3848.250 kb on - strand, within uhpA at 3848.261 kb on + strand, within uhpA at 3848.285 kb on - strand, within uhpA at 3848.370 kb on - strand, within uhpA at 3848.439 kb on + strand, within uhpA at 3848.492 kb on - strand, within uhpA at 3848.492 kb on - strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.504 kb on + strand, within uhpA at 3848.504 kb on + strand, within uhpA at 3848.524 kb on - strand, within uhpA at 3848.524 kb on - strand, within uhpA at 3848.524 kb on - strand, within uhpA at 3848.526 kb on - strand, within uhpA at 3848.565 kb on - strand, within uhpA at 3848.565 kb on - strand, within uhpA at 3848.571 kb on + strand, within uhpA at 3848.571 kb on + strand, within uhpA at 3848.577 kb on + strand, within uhpA at 3848.585 kb on - strand, within uhpA at 3848.595 kb on + strand, within uhpA at 3848.603 kb on - strand, within uhpA at 3848.608 kb on - strand, within uhpA at 3848.610 kb on - strand, within uhpA at 3848.719 kb on + strand at 3848.734 kb on + strand at 3848.904 kb on - strand, within ilvN at 3848.921 kb on + strand, within ilvN at 3848.950 kb on + strand, within ilvN at 3848.953 kb on + strand, within ilvN at 3848.953 kb on + strand, within ilvN at 3848.958 kb on - strand, within ilvN at 3848.958 kb on - strand, within ilvN at 3848.966 kb on + strand, within ilvN at 3848.978 kb on + strand, within ilvN at 3848.978 kb on + strand, within ilvN at 3848.986 kb on - strand, within ilvN at 3849.000 kb on - strand, within ilvN at 3849.000 kb on - strand, within ilvN at 3849.044 kb on - strand, within ilvN at 3849.044 kb on - strand, within ilvN at 3849.044 kb on - strand, within ilvN at 3849.050 kb on + strand, within ilvN at 3849.050 kb on + strand, within ilvN at 3849.055 kb on - strand, within ilvN at 3849.103 kb on - strand at 3849.103 kb on - strand at 3849.110 kb on - strand at 3849.140 kb on - strand at 3849.193 kb on + strand at 3849.391 kb on - strand, within ilvB at 3849.404 kb on - strand, within ilvB at 3849.404 kb on - strand, within ilvB at 3849.467 kb on - strand, within ilvB at 3849.485 kb on - strand, within ilvB at 3849.496 kb on - strand, within ilvB at 3849.517 kb on + strand, within ilvB at 3849.568 kb on - strand, within ilvB at 3849.576 kb on + strand, within ilvB at 3849.578 kb on + strand, within ilvB at 3849.598 kb on + strand, within ilvB at 3849.605 kb on + strand, within ilvB at 3849.615 kb on + strand, within ilvB at 3849.617 kb on + strand, within ilvB at 3849.617 kb on + strand, within ilvB at 3849.626 kb on + strand, within ilvB at 3849.636 kb on + strand, within ilvB at 3849.775 kb on - strand, within ilvB at 3849.828 kb on - strand, within ilvB at 3849.846 kb on + strand, within ilvB at 3849.917 kb on + strand, within ilvB at 3849.917 kb on + strand, within ilvB at 3850.001 kb on - strand, within ilvB at 3850.024 kb on - strand, within ilvB at 3850.027 kb on + strand, within ilvB at 3850.027 kb on + strand, within ilvB at 3850.027 kb on + strand, within ilvB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,847,825 - uhpB b3668 0.78 +0.1 3,847,825 - uhpB b3668 0.78 +0.3 3,847,828 - uhpB b3668 0.78 +0.2 3,847,828 - uhpB b3668 0.78 +0.2 3,847,880 - uhpB b3668 0.81 -0.1 3,847,883 - uhpB b3668 0.82 +0.3 3,847,883 - uhpB b3668 0.82 -0.4 3,847,942 + uhpB b3668 0.85 -0.5 3,848,011 - +1.4 3,848,016 - +0.5 3,848,029 - -0.1 3,848,029 - -0.4 3,848,043 + +0.1 3,848,045 + -0.9 3,848,047 + +0.1 3,848,074 - +0.7 3,848,074 - -0.6 3,848,083 - +1.1 3,848,094 - +0.3 3,848,129 + -0.0 3,848,129 + +0.3 3,848,163 - -0.2 3,848,165 + +0.1 3,848,193 + +0.4 3,848,193 + +0.2 3,848,196 - -0.4 3,848,237 + uhpA b3669 0.13 -1.3 3,848,250 - uhpA b3669 0.15 +1.7 3,848,261 + uhpA b3669 0.17 -0.2 3,848,285 - uhpA b3669 0.21 -1.2 3,848,370 - uhpA b3669 0.36 -1.1 3,848,439 + uhpA b3669 0.47 -1.7 3,848,492 - uhpA b3669 0.56 -0.1 3,848,492 - uhpA b3669 0.56 +0.4 3,848,494 + uhpA b3669 0.57 -1.3 3,848,494 + uhpA b3669 0.57 -1.4 3,848,494 + uhpA b3669 0.57 +0.4 3,848,494 + uhpA b3669 0.57 -0.0 3,848,504 + uhpA b3669 0.58 +0.4 3,848,504 + uhpA b3669 0.58 +0.1 3,848,524 - uhpA b3669 0.62 +1.4 3,848,524 - uhpA b3669 0.62 -0.9 3,848,524 - uhpA b3669 0.62 +0.6 3,848,526 - uhpA b3669 0.62 +0.8 3,848,565 - uhpA b3669 0.69 -0.4 3,848,565 - uhpA b3669 0.69 -1.9 3,848,571 + uhpA b3669 0.70 -0.5 3,848,571 + uhpA b3669 0.70 -0.2 3,848,577 + uhpA b3669 0.71 +0.1 3,848,585 - uhpA b3669 0.72 -0.9 3,848,595 + uhpA b3669 0.74 +0.2 3,848,603 - uhpA b3669 0.75 -0.2 3,848,608 - uhpA b3669 0.76 +0.3 3,848,610 - uhpA b3669 0.76 -0.6 3,848,719 + +1.1 3,848,734 + +0.4 3,848,904 - ilvN b3670 0.27 -1.4 3,848,921 + ilvN b3670 0.33 -3.5 3,848,950 + ilvN b3670 0.43 -0.8 3,848,953 + ilvN b3670 0.44 -1.9 3,848,953 + ilvN b3670 0.44 +0.7 3,848,958 - ilvN b3670 0.46 -0.4 3,848,958 - ilvN b3670 0.46 -1.6 3,848,966 + ilvN b3670 0.48 -1.8 3,848,978 + ilvN b3670 0.53 -1.3 3,848,978 + ilvN b3670 0.53 -1.7 3,848,986 - ilvN b3670 0.55 -1.8 3,849,000 - ilvN b3670 0.60 -0.3 3,849,000 - ilvN b3670 0.60 -1.7 3,849,044 - ilvN b3670 0.75 -1.5 3,849,044 - ilvN b3670 0.75 -1.2 3,849,044 - ilvN b3670 0.75 -0.9 3,849,050 + ilvN b3670 0.77 -2.1 3,849,050 + ilvN b3670 0.77 -3.0 3,849,055 - ilvN b3670 0.79 -0.5 3,849,103 - -0.2 3,849,103 - -0.9 3,849,110 - +0.0 3,849,140 - -0.9 3,849,193 + -1.9 3,849,391 - ilvB b3671 0.16 -3.0 3,849,404 - ilvB b3671 0.17 -1.5 3,849,404 - ilvB b3671 0.17 -2.1 3,849,467 - ilvB b3671 0.21 -5.0 3,849,485 - ilvB b3671 0.22 -2.8 3,849,496 - ilvB b3671 0.22 -4.1 3,849,517 + ilvB b3671 0.24 -2.4 3,849,568 - ilvB b3671 0.27 -0.3 3,849,576 + ilvB b3671 0.27 -3.1 3,849,578 + ilvB b3671 0.27 -3.5 3,849,598 + ilvB b3671 0.28 -2.9 3,849,605 + ilvB b3671 0.29 -3.6 3,849,615 + ilvB b3671 0.29 -1.9 3,849,617 + ilvB b3671 0.29 -0.9 3,849,617 + ilvB b3671 0.29 -1.7 3,849,626 + ilvB b3671 0.30 -2.4 3,849,636 + ilvB b3671 0.31 -3.5 3,849,775 - ilvB b3671 0.39 -1.9 3,849,828 - ilvB b3671 0.42 -3.7 3,849,846 + ilvB b3671 0.43 -1.7 3,849,917 + ilvB b3671 0.47 -2.7 3,849,917 + ilvB b3671 0.47 -2.6 3,850,001 - ilvB b3671 0.52 -1.9 3,850,024 - ilvB b3671 0.54 -2.2 3,850,027 + ilvB b3671 0.54 -1.2 3,850,027 + ilvB b3671 0.54 -0.7 3,850,027 + ilvB b3671 0.54 -2.0
Or see this region's nucleotide sequence