Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4250 and EX28DRAFT_4251 are separated by 206 nucleotides EX28DRAFT_4251 and EX28DRAFT_4252 are separated by 75 nucleotides EX28DRAFT_4252 and EX28DRAFT_4253 overlap by 1 nucleotides
EX28DRAFT_4250: EX28DRAFT_4250 - Aspartate/tyrosine/aromatic aminotransferase, at 57,225 to 58,418
_4250
EX28DRAFT_4251: EX28DRAFT_4251 - Protein of unknown function (DUF2589), at 58,625 to 59,182
_4251
EX28DRAFT_4252: EX28DRAFT_4252 - Protein of unknown function (DUF2589), at 59,258 to 59,860
_4252
EX28DRAFT_4253: EX28DRAFT_4253 - Phage-related lysozyme (muraminidase), at 59,860 to 60,309
_4253
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-2
-1
0
1 at 57.775 kb on + strand, within EX28DRAFT_4250 at 57.816 kb on + strand, within EX28DRAFT_4250 at 57.817 kb on - strand, within EX28DRAFT_4250 at 58.066 kb on + strand, within EX28DRAFT_4250 at 58.067 kb on - strand, within EX28DRAFT_4250 at 58.223 kb on - strand, within EX28DRAFT_4250 at 58.293 kb on - strand, within EX28DRAFT_4250 at 58.309 kb on + strand at 58.309 kb on + strand at 58.313 kb on - strand at 58.313 kb on - strand at 58.325 kb on + strand at 58.325 kb on + strand at 58.383 kb on - strand at 58.461 kb on + strand at 58.462 kb on - strand at 58.462 kb on - strand at 58.514 kb on - strand at 58.552 kb on - strand at 58.640 kb on - strand at 58.651 kb on + strand at 58.675 kb on + strand at 58.676 kb on - strand at 58.676 kb on - strand at 58.676 kb on - strand at 58.676 kb on - strand at 58.733 kb on + strand, within EX28DRAFT_4251 at 58.831 kb on + strand, within EX28DRAFT_4251 at 59.066 kb on - strand, within EX28DRAFT_4251 at 59.089 kb on + strand, within EX28DRAFT_4251 at 59.098 kb on + strand, within EX28DRAFT_4251 at 59.141 kb on + strand at 59.141 kb on + strand at 59.141 kb on + strand at 59.142 kb on - strand at 59.200 kb on + strand at 59.200 kb on + strand at 59.200 kb on + strand at 59.201 kb on - strand at 59.408 kb on - strand, within EX28DRAFT_4252 at 59.408 kb on - strand, within EX28DRAFT_4252 at 59.426 kb on + strand, within EX28DRAFT_4252 at 59.465 kb on + strand, within EX28DRAFT_4252 at 59.465 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.467 kb on + strand, within EX28DRAFT_4252 at 59.468 kb on - strand, within EX28DRAFT_4252 at 59.814 kb on - strand at 59.880 kb on + strand at 59.881 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 57,775 + EX28DRAFT_4250 0.46 -0.3 57,816 + EX28DRAFT_4250 0.49 +0.0 57,817 - EX28DRAFT_4250 0.50 -0.5 58,066 + EX28DRAFT_4250 0.70 -0.6 58,067 - EX28DRAFT_4250 0.71 -0.6 58,223 - EX28DRAFT_4250 0.84 -0.0 58,293 - EX28DRAFT_4250 0.89 -2.0 58,309 + +0.1 58,309 + -2.7 58,313 - -0.2 58,313 - -0.7 58,325 + -0.7 58,325 + -1.4 58,383 - -0.3 58,461 + -0.3 58,462 - +0.2 58,462 - +1.6 58,514 - +0.3 58,552 - -1.1 58,640 - +0.4 58,651 + +0.3 58,675 + +0.6 58,676 - +0.9 58,676 - -0.4 58,676 - +0.6 58,676 - +0.4 58,733 + EX28DRAFT_4251 0.19 +0.2 58,831 + EX28DRAFT_4251 0.37 -1.5 59,066 - EX28DRAFT_4251 0.79 -0.5 59,089 + EX28DRAFT_4251 0.83 -0.9 59,098 + EX28DRAFT_4251 0.85 +0.3 59,141 + +0.9 59,141 + +0.4 59,141 + -0.7 59,142 - +0.4 59,200 + -0.3 59,200 + -0.0 59,200 + +0.3 59,201 - +1.5 59,408 - EX28DRAFT_4252 0.25 +0.0 59,408 - EX28DRAFT_4252 0.25 -1.4 59,426 + EX28DRAFT_4252 0.28 +0.4 59,465 + EX28DRAFT_4252 0.34 +0.0 59,465 + EX28DRAFT_4252 0.34 -0.7 59,467 + EX28DRAFT_4252 0.35 +0.3 59,467 + EX28DRAFT_4252 0.35 +0.4 59,467 + EX28DRAFT_4252 0.35 -0.7 59,467 + EX28DRAFT_4252 0.35 -0.0 59,468 - EX28DRAFT_4252 0.35 -1.8 59,814 - -0.5 59,880 + -0.0 59,881 - -0.1
Or see this region's nucleotide sequence