Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3391 and EX28DRAFT_3392 are separated by 96 nucleotides EX28DRAFT_3392 and EX28DRAFT_3393 are separated by 244 nucleotides
EX28DRAFT_3391: EX28DRAFT_3391 - Gamma-aminobutyrate permease and related permeases, at 58,145 to 59,554
_3391
EX28DRAFT_3392: EX28DRAFT_3392 - iron-sulfur cluster repair di-iron protein, at 59,651 to 60,313
_3392
EX28DRAFT_3393: EX28DRAFT_3393 - methyl-accepting chemotaxis sensory transducer with TarH sensor, at 60,558 to 62,207
_3393
Position (kb)
59
60
61 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 58.735 kb on + strand, within EX28DRAFT_3391 at 58.809 kb on + strand, within EX28DRAFT_3391 at 58.809 kb on + strand, within EX28DRAFT_3391 at 58.892 kb on - strand, within EX28DRAFT_3391 at 58.910 kb on - strand, within EX28DRAFT_3391 at 58.910 kb on - strand, within EX28DRAFT_3391 at 58.922 kb on + strand, within EX28DRAFT_3391 at 58.923 kb on - strand, within EX28DRAFT_3391 at 58.923 kb on - strand, within EX28DRAFT_3391 at 58.923 kb on - strand, within EX28DRAFT_3391 at 58.923 kb on - strand, within EX28DRAFT_3391 at 58.946 kb on - strand, within EX28DRAFT_3391 at 58.946 kb on - strand, within EX28DRAFT_3391 at 59.014 kb on - strand, within EX28DRAFT_3391 at 59.041 kb on + strand, within EX28DRAFT_3391 at 59.041 kb on + strand, within EX28DRAFT_3391 at 59.042 kb on - strand, within EX28DRAFT_3391 at 59.231 kb on + strand, within EX28DRAFT_3391 at 59.232 kb on - strand, within EX28DRAFT_3391 at 59.263 kb on + strand, within EX28DRAFT_3391 at 59.294 kb on - strand, within EX28DRAFT_3391 at 59.294 kb on - strand, within EX28DRAFT_3391 at 59.334 kb on - strand, within EX28DRAFT_3391 at 59.372 kb on + strand, within EX28DRAFT_3391 at 59.372 kb on + strand, within EX28DRAFT_3391 at 59.373 kb on - strand, within EX28DRAFT_3391 at 59.536 kb on + strand at 59.553 kb on - strand at 59.553 kb on - strand at 59.663 kb on + strand at 59.723 kb on - strand, within EX28DRAFT_3392 at 59.742 kb on + strand, within EX28DRAFT_3392 at 59.743 kb on - strand, within EX28DRAFT_3392 at 59.891 kb on + strand, within EX28DRAFT_3392 at 59.892 kb on - strand, within EX28DRAFT_3392 at 59.927 kb on - strand, within EX28DRAFT_3392 at 59.927 kb on - strand, within EX28DRAFT_3392 at 59.961 kb on + strand, within EX28DRAFT_3392 at 60.062 kb on - strand, within EX28DRAFT_3392 at 60.241 kb on - strand, within EX28DRAFT_3392 at 60.241 kb on - strand, within EX28DRAFT_3392 at 60.314 kb on - strand at 60.335 kb on - strand at 60.374 kb on + strand at 60.375 kb on - strand at 60.375 kb on - strand at 60.375 kb on - strand at 60.451 kb on + strand at 60.451 kb on + strand at 60.452 kb on - strand at 60.452 kb on - strand at 60.452 kb on - strand at 60.484 kb on - strand at 60.484 kb on - strand at 60.516 kb on + strand at 60.518 kb on + strand at 60.519 kb on - strand at 60.519 kb on - strand at 60.541 kb on + strand at 60.541 kb on + strand at 60.542 kb on - strand at 60.542 kb on - strand at 60.542 kb on - strand at 60.542 kb on - strand at 60.543 kb on + strand at 60.543 kb on + strand at 60.544 kb on - strand at 60.544 kb on - strand at 60.569 kb on + strand at 60.570 kb on - strand at 60.570 kb on - strand at 60.571 kb on + strand at 60.875 kb on + strand, within EX28DRAFT_3393 at 60.876 kb on - strand, within EX28DRAFT_3393 at 60.879 kb on + strand, within EX28DRAFT_3393 at 60.879 kb on + strand, within EX28DRAFT_3393 at 60.879 kb on + strand, within EX28DRAFT_3393 at 60.880 kb on - strand, within EX28DRAFT_3393 at 60.880 kb on - strand, within EX28DRAFT_3393 at 60.880 kb on - strand, within EX28DRAFT_3393 at 60.880 kb on - strand, within EX28DRAFT_3393 at 60.880 kb on - strand, within EX28DRAFT_3393 at 60.880 kb on - strand, within EX28DRAFT_3393 at 60.880 kb on - strand, within EX28DRAFT_3393 at 60.882 kb on + strand, within EX28DRAFT_3393 at 60.882 kb on + strand, within EX28DRAFT_3393 at 60.882 kb on + strand, within EX28DRAFT_3393 at 60.882 kb on + strand, within EX28DRAFT_3393 at 60.882 kb on + strand, within EX28DRAFT_3393 at 60.882 kb on + strand, within EX28DRAFT_3393 at 60.883 kb on - strand, within EX28DRAFT_3393 at 61.007 kb on + strand, within EX28DRAFT_3393 at 61.123 kb on - strand, within EX28DRAFT_3393 at 61.178 kb on + strand, within EX28DRAFT_3393 at 61.292 kb on + strand, within EX28DRAFT_3393 at 61.292 kb on + strand, within EX28DRAFT_3393 at 61.293 kb on - strand, within EX28DRAFT_3393 at 61.293 kb on - strand, within EX28DRAFT_3393 at 61.293 kb on - strand, within EX28DRAFT_3393
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 58,735 + EX28DRAFT_3391 0.42 -0.6 58,809 + EX28DRAFT_3391 0.47 +0.1 58,809 + EX28DRAFT_3391 0.47 -1.4 58,892 - EX28DRAFT_3391 0.53 +0.2 58,910 - EX28DRAFT_3391 0.54 +0.5 58,910 - EX28DRAFT_3391 0.54 -0.4 58,922 + EX28DRAFT_3391 0.55 +0.1 58,923 - EX28DRAFT_3391 0.55 -0.4 58,923 - EX28DRAFT_3391 0.55 +0.1 58,923 - EX28DRAFT_3391 0.55 -0.4 58,923 - EX28DRAFT_3391 0.55 +1.6 58,946 - EX28DRAFT_3391 0.57 +0.7 58,946 - EX28DRAFT_3391 0.57 +0.2 59,014 - EX28DRAFT_3391 0.62 +0.1 59,041 + EX28DRAFT_3391 0.64 -0.8 59,041 + EX28DRAFT_3391 0.64 -0.1 59,042 - EX28DRAFT_3391 0.64 -1.0 59,231 + EX28DRAFT_3391 0.77 -0.1 59,232 - EX28DRAFT_3391 0.77 +0.4 59,263 + EX28DRAFT_3391 0.79 -2.8 59,294 - EX28DRAFT_3391 0.81 -0.1 59,294 - EX28DRAFT_3391 0.81 -0.5 59,334 - EX28DRAFT_3391 0.84 -0.4 59,372 + EX28DRAFT_3391 0.87 +0.1 59,372 + EX28DRAFT_3391 0.87 +0.0 59,373 - EX28DRAFT_3391 0.87 +0.2 59,536 + +0.7 59,553 - -0.0 59,553 - -0.4 59,663 + +0.3 59,723 - EX28DRAFT_3392 0.11 +0.8 59,742 + EX28DRAFT_3392 0.14 -0.1 59,743 - EX28DRAFT_3392 0.14 +0.5 59,891 + EX28DRAFT_3392 0.36 -0.3 59,892 - EX28DRAFT_3392 0.36 -0.0 59,927 - EX28DRAFT_3392 0.42 +0.9 59,927 - EX28DRAFT_3392 0.42 -0.3 59,961 + EX28DRAFT_3392 0.47 +1.3 60,062 - EX28DRAFT_3392 0.62 -0.7 60,241 - EX28DRAFT_3392 0.89 +1.0 60,241 - EX28DRAFT_3392 0.89 +0.6 60,314 - +0.4 60,335 - -2.4 60,374 + +0.1 60,375 - -1.5 60,375 - -1.2 60,375 - +0.8 60,451 + -1.1 60,451 + -1.8 60,452 - +0.5 60,452 - -0.3 60,452 - +0.2 60,484 - +0.1 60,484 - +0.1 60,516 + +0.2 60,518 + -0.1 60,519 - -0.2 60,519 - -0.4 60,541 + -0.3 60,541 + +0.1 60,542 - +0.2 60,542 - +0.9 60,542 - +0.0 60,542 - +1.0 60,543 + -0.1 60,543 + -0.2 60,544 - +0.2 60,544 - -1.1 60,569 + -1.0 60,570 - +1.8 60,570 - -0.8 60,571 + +0.5 60,875 + EX28DRAFT_3393 0.19 +0.0 60,876 - EX28DRAFT_3393 0.19 -1.4 60,879 + EX28DRAFT_3393 0.19 -0.1 60,879 + EX28DRAFT_3393 0.19 -0.6 60,879 + EX28DRAFT_3393 0.19 -0.2 60,880 - EX28DRAFT_3393 0.20 +0.1 60,880 - EX28DRAFT_3393 0.20 +0.3 60,880 - EX28DRAFT_3393 0.20 -0.2 60,880 - EX28DRAFT_3393 0.20 -1.0 60,880 - EX28DRAFT_3393 0.20 +0.1 60,880 - EX28DRAFT_3393 0.20 -0.3 60,880 - EX28DRAFT_3393 0.20 -0.1 60,882 + EX28DRAFT_3393 0.20 +0.4 60,882 + EX28DRAFT_3393 0.20 -1.3 60,882 + EX28DRAFT_3393 0.20 +0.3 60,882 + EX28DRAFT_3393 0.20 +0.3 60,882 + EX28DRAFT_3393 0.20 -1.1 60,882 + EX28DRAFT_3393 0.20 -0.4 60,883 - EX28DRAFT_3393 0.20 +0.1 61,007 + EX28DRAFT_3393 0.27 +0.2 61,123 - EX28DRAFT_3393 0.34 -0.7 61,178 + EX28DRAFT_3393 0.38 -0.4 61,292 + EX28DRAFT_3393 0.44 +2.1 61,292 + EX28DRAFT_3393 0.44 -0.9 61,293 - EX28DRAFT_3393 0.45 -0.3 61,293 - EX28DRAFT_3393 0.45 +0.1 61,293 - EX28DRAFT_3393 0.45 -0.8
Or see this region's nucleotide sequence