Experiment: M9 with sucrose; no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3345 and EX28DRAFT_3346 are separated by 37 nucleotides EX28DRAFT_3346 and EX28DRAFT_3347 are separated by 189 nucleotides EX28DRAFT_3347 and EX28DRAFT_3348 are separated by 27 nucleotides EX28DRAFT_3348 and EX28DRAFT_3349 overlap by 4 nucleotides EX28DRAFT_3349 and EX28DRAFT_3350 are separated by 138 nucleotides
EX28DRAFT_3345: EX28DRAFT_3345 - Predicted small secreted protein, at 14,630 to 14,776
_3345
EX28DRAFT_3346: EX28DRAFT_3346 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 14,814 to 15,413
_3346
EX28DRAFT_3347: EX28DRAFT_3347 - RNA, at 15,603 to 15,647
_3347
EX28DRAFT_3348: EX28DRAFT_3348 - Membrane transporters of cations and cationic drugs, at 15,675 to 15,992
_3348
EX28DRAFT_3349: EX28DRAFT_3349 - Bacterial lipocalin, at 15,989 to 16,519
_3349
EX28DRAFT_3350: EX28DRAFT_3350 - Beta-lactamase class C and other penicillin binding proteins, at 16,658 to 17,800
_3350
Position (kb) 15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 14.695 kb on + strand, within EX28DRAFT_3345 at 14.698 kb on + strand, within EX28DRAFT_3345 at 14.761 kb on + strand, within EX28DRAFT_3345 at 14.774 kb on + strand at 14.775 kb on - strand at 14.778 kb on + strand at 14.783 kb on + strand at 14.784 kb on - strand at 14.784 kb on - strand at 14.784 kb on - strand at 14.785 kb on - strand at 14.893 kb on + strand, within EX28DRAFT_3346 at 14.893 kb on + strand, within EX28DRAFT_3346 at 14.893 kb on + strand, within EX28DRAFT_3346 at 14.894 kb on - strand, within EX28DRAFT_3346 at 14.897 kb on + strand, within EX28DRAFT_3346 at 14.897 kb on + strand, within EX28DRAFT_3346 at 14.897 kb on + strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.898 kb on - strand, within EX28DRAFT_3346 at 14.899 kb on + strand, within EX28DRAFT_3346 at 14.900 kb on - strand, within EX28DRAFT_3346 at 14.900 kb on - strand, within EX28DRAFT_3346 at 15.222 kb on - strand, within EX28DRAFT_3346 at 15.322 kb on + strand, within EX28DRAFT_3346 at 15.322 kb on + strand, within EX28DRAFT_3346 at 15.413 kb on + strand at 15.471 kb on + strand at 15.471 kb on + strand at 15.492 kb on - strand at 15.492 kb on - strand at 15.514 kb on - strand at 15.523 kb on - strand at 15.534 kb on + strand at 15.584 kb on + strand at 15.654 kb on + strand at 15.654 kb on + strand at 15.655 kb on - strand at 15.657 kb on + strand at 15.657 kb on + strand at 15.658 kb on - strand at 15.658 kb on - strand at 15.695 kb on + strand at 15.719 kb on - strand, within EX28DRAFT_3348 at 15.775 kb on + strand, within EX28DRAFT_3348 at 15.775 kb on + strand, within EX28DRAFT_3348 at 15.800 kb on + strand, within EX28DRAFT_3348 at 15.800 kb on + strand, within EX28DRAFT_3348 at 15.800 kb on + strand, within EX28DRAFT_3348 at 15.801 kb on - strand, within EX28DRAFT_3348 at 15.812 kb on - strand, within EX28DRAFT_3348 at 15.849 kb on + strand, within EX28DRAFT_3348 at 16.081 kb on + strand, within EX28DRAFT_3349 at 16.173 kb on + strand, within EX28DRAFT_3349 at 16.283 kb on - strand, within EX28DRAFT_3349 at 16.329 kb on + strand, within EX28DRAFT_3349 at 16.329 kb on + strand, within EX28DRAFT_3349 at 16.349 kb on + strand, within EX28DRAFT_3349 at 16.350 kb on - strand, within EX28DRAFT_3349 at 16.389 kb on + strand, within EX28DRAFT_3349 at 16.390 kb on - strand, within EX28DRAFT_3349 at 16.463 kb on - strand, within EX28DRAFT_3349 at 16.519 kb on + strand at 16.519 kb on + strand at 16.520 kb on - strand at 16.545 kb on + strand at 16.552 kb on - strand at 16.656 kb on + strand at 16.656 kb on + strand at 16.660 kb on - strand at 16.660 kb on - strand at 16.768 kb on - strand at 16.768 kb on - strand at 16.768 kb on - strand at 16.768 kb on - strand at 16.798 kb on + strand, within EX28DRAFT_3350 at 16.874 kb on - strand, within EX28DRAFT_3350 at 16.919 kb on - strand, within EX28DRAFT_3350 at 16.944 kb on + strand, within EX28DRAFT_3350 at 16.945 kb on - strand, within EX28DRAFT_3350 at 16.945 kb on - strand, within EX28DRAFT_3350
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose; no stress control remove 14,695 + EX28DRAFT_3345 0.44 +1.0 14,698 + EX28DRAFT_3345 0.46 -0.0 14,761 + EX28DRAFT_3345 0.89 +0.3 14,774 + -0.1 14,775 - +3.0 14,778 + -0.4 14,783 + +0.1 14,784 - -0.3 14,784 - +0.4 14,784 - -0.6 14,785 - -0.3 14,893 + EX28DRAFT_3346 0.13 -1.4 14,893 + EX28DRAFT_3346 0.13 -0.1 14,893 + EX28DRAFT_3346 0.13 -0.4 14,894 - EX28DRAFT_3346 0.13 +0.7 14,897 + EX28DRAFT_3346 0.14 +0.3 14,897 + EX28DRAFT_3346 0.14 -0.0 14,897 + EX28DRAFT_3346 0.14 +0.1 14,898 - EX28DRAFT_3346 0.14 -0.1 14,898 - EX28DRAFT_3346 0.14 -0.1 14,898 - EX28DRAFT_3346 0.14 +1.1 14,898 - EX28DRAFT_3346 0.14 +1.5 14,899 + EX28DRAFT_3346 0.14 +0.3 14,900 - EX28DRAFT_3346 0.14 +0.2 14,900 - EX28DRAFT_3346 0.14 -0.3 15,222 - EX28DRAFT_3346 0.68 -0.4 15,322 + EX28DRAFT_3346 0.85 -0.3 15,322 + EX28DRAFT_3346 0.85 -1.4 15,413 + +0.4 15,471 + +0.1 15,471 + -0.1 15,492 - -0.4 15,492 - +0.0 15,514 - -0.0 15,523 - +0.1 15,534 + -0.8 15,584 + +0.8 15,654 + -0.7 15,654 + +0.4 15,655 - -0.3 15,657 + +0.1 15,657 + +0.4 15,658 - -0.7 15,658 - -1.0 15,695 + -0.3 15,719 - EX28DRAFT_3348 0.14 +0.2 15,775 + EX28DRAFT_3348 0.31 +1.2 15,775 + EX28DRAFT_3348 0.31 +0.9 15,800 + EX28DRAFT_3348 0.39 +0.0 15,800 + EX28DRAFT_3348 0.39 +0.0 15,800 + EX28DRAFT_3348 0.39 +0.7 15,801 - EX28DRAFT_3348 0.40 +0.4 15,812 - EX28DRAFT_3348 0.43 -0.4 15,849 + EX28DRAFT_3348 0.55 +0.4 16,081 + EX28DRAFT_3349 0.17 +0.4 16,173 + EX28DRAFT_3349 0.35 -0.0 16,283 - EX28DRAFT_3349 0.55 +0.1 16,329 + EX28DRAFT_3349 0.64 -0.4 16,329 + EX28DRAFT_3349 0.64 +0.0 16,349 + EX28DRAFT_3349 0.68 +0.4 16,350 - EX28DRAFT_3349 0.68 -0.1 16,389 + EX28DRAFT_3349 0.75 -0.2 16,390 - EX28DRAFT_3349 0.76 -0.6 16,463 - EX28DRAFT_3349 0.89 +0.1 16,519 + -3.6 16,519 + +0.7 16,520 - -0.2 16,545 + -0.5 16,552 - +2.5 16,656 + -0.1 16,656 + +1.7 16,660 - -1.8 16,660 - +0.2 16,768 - -0.3 16,768 - -0.5 16,768 - +0.2 16,768 - -0.0 16,798 + EX28DRAFT_3350 0.12 +0.2 16,874 - EX28DRAFT_3350 0.19 -0.5 16,919 - EX28DRAFT_3350 0.23 +0.2 16,944 + EX28DRAFT_3350 0.25 +1.5 16,945 - EX28DRAFT_3350 0.25 -1.9 16,945 - EX28DRAFT_3350 0.25 -0.8
Or see this region's nucleotide sequence